Plasmodium falciparum and Helminth Coinfections Increase IgE and Parasite-Specific IgG Responses

Santano, Rebeca; Rubio, Rocío; Grau-Pujol, Berta; Escola, Valdemiro; Muchisse, Osvaldo; Cuamba, Inocencia; Vidal, Marta; Cisteró, Pau; Ruiz‐Olalla, Gemma; Aguilar, Ruth; Demontis, Maria Piera; Jamine, Jose Carlos; Cossa, Anélsio; Sacoor, Charfudin; Cano, Jorge; Izquierdo, Luís; Chitnis, Chetan E.; Coppel, Ross L.; Chauhan, Virander S.; Cavanagh, David; Dutta, Sandeep; Angov, Evelina; Gaur, Deepak; Lieshout, Lisette van; Zhan, Bin; Muñóz, José; Moncunill, Gemma; Dobaño, Carlota

doi:10.1128/spectrum.01109-21

Cited by 13 publications

(14 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although P. falciparum does not appear to have any effect on H. nana intensity and vice versa, co-infection of P. falciparum with H. nana presented a grievous impairment to the anti-GMZ2 IgG response, which is consistent with experimental evidence that implicates H. nana , a human tapeworm, and rodent one (Hymenolepis diminuta) in dampening the immune response ( 33 ). This, however, contrasts sharply with a couple of earlier reports ( 21 , 34 ). The differential outcome may be attributed to the study designs as helminth positive groups reported, and age of the study participants which influences parasites carriage and immunity to either group of parasites ( 34 ) could have impacted on the results.…”

Section: Discussioncontrasting

confidence: 99%

Humoral antimalaria immune response in Nigerian children exposed to helminth and malaria parasites

et al. 2022

View full text Add to dashboard Cite

BackgroundMalaria and helminthic parasites are endemic in tropical countries, and co-infections might influence host-parasite interactions. In this community-based cross-sectional study, the effect that the presence of soil-transmitted helminths (STH) (Hookworm, Hymenolepis nana) and Schistosoma haematobium infections could have on the immunoglobulin (Ig) candidate protein of the malaria vaccine GMZ2 levels was evaluated.MethodsBlood, stool, and urine samples were collected from 5-15-year-old children to diagnose P. falciparum (Pf), STH, and Schistosoma haematobium, respectively. Identification and quantification of the parasite load of STH and S. haematobium were achieved by light microscopy. A polymerase chain reaction was carried out to detect submicroscopic infections of P. falciparum. Plasma levels of GMZ2 specific IgG and its subclasses were quantified by ELISA.ResultsThe median level of total IgG in individuals with co-infection with Pf/H. nana was significantly lower in the mono-infected group with Pf (p = 0.0121) or study participants without infection (p=0.0217). Similarly, the median level of IgG1 was statistically lower in Pf/H. nana group compared to Pf-group (p=0.0137). Equally, the Pf/H. nana infected individuals posted a lower level of IgG1 compared to Pf-group (p=0.0137) and IgG4 compared to the Pf-group (p=0.0144). Spearman rank correlation analyses indicated positive relationships between the densities of H. nana (ρ=0.25, p=0.015) and S. haematobium (ρ=0.36, p<0.0001).ConclusionsHookworm and H. nana infections are associated with reduced GMZ2 specific IgG levels. This study shows the possible manipulation of immune responses by helminths for their survival and transmission, which may have serious implications for vaccine development and deployment in helminth-endemic regions.

show abstract

Section: Discussioncontrasting

confidence: 99%

Humoral antimalaria immune response in Nigerian children exposed to helminth and malaria parasites

et al. 2022

View full text Add to dashboard Cite

show abstract

“…P . falciparum infection was determined at the Barcelona Institute for Global Health (ISGlobal) (Spain) by 18S ribosomal RNA gene detection through qPCR from dried blood spot (DBS) samples as previously described [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

“…A detailed explanation of the antigen panel and serum elution procedure from DBS used for this analysis is described in Santano et al . 2021 [ 28 ]. Eluted serum was used to measure total IgE and antigen-specific IgG by quantitative suspension array technology (qSAT) using xMAP Luminex technology against a panel of 27 antigens: 16 from P .…”

Section: Methodsmentioning

confidence: 99%

“…The receiver operating characteristic (ROC) curves and their corresponding areas under the curve (AUC) for each antibody response were built with the R CRAN package pROC [ 37 ] using the log 10 -transformed MFIs as predictor and the microscopic, molecular or combination of both diagnoses as response variable. Since antibodies are acquired over time [ 28 ], we also performed the analysis stratified by age group (children and adults). In addition, to eliminate the possible interference of helminths and P .…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, we have recently shown that this interaction might be bidirectional, since it was observed that exposure to or co-infection with helminths and P . falciparum was associated with an increase in antibody responses to antigens from both pathogens [ 28 ]. Given the reported bidirectional impact on the immune responses to both parasitic infections, it is relevant to study antibody serological diagnostic methods in a context where co-infections might occur and affect the accuracy of the diagnosis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure. Methodology We evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference. Principal findings IgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure although sensitivity could not be assessed. Conclusions We provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers have potential good specificity as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens.

show abstract

Enhanced protective immunity against Baylisascaris schroederi infection in mice through a multi-antigen cocktail vaccine approach

Xiong,

Chen,

Chen

et al. 2023

Parasitol Res

View full text Add to dashboard Cite

Plasmodium falciparum and Helminth Coinfections Increase IgE and Parasite-Specific IgG Responses

Cited by 13 publications

References 77 publications

Humoral antimalaria immune response in Nigerian children exposed to helminth and malaria parasites

Humoral antimalaria immune response in Nigerian children exposed to helminth and malaria parasites

Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique

Enhanced protective immunity against Baylisascaris schroederi infection in mice through a multi-antigen cocktail vaccine approach

Contact Info

Product

Resources

About