Plasmodium chabaudi: Adoptive transfer of immunity with different spleen cell populations and development of protective activity in the serum of lethally irradiated recipient mice

McDonald, Vincent; Phillips, R. S.

doi:10.1016/0014-4894(80)90052-1

Cited by 9 publications

(3 citation statements)

References 19 publications

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“…Similar results were shown in previous reports (Cox and Voller 1966;Cox 1970;McLean et al 1982). Experiments dealing with cell transfer of immunity have thus far usually used immunocompromised hosts such as nude mice (Cavacini et al 1986;Brake et al 1988;Meding and Langhorne 1991), SCID mice (Meding and Langhorne 1991), and irradiated mice (McDonald and Phillips 1980;Favila-Castillo et al 1990;Legorreta-Herrera et al 1993) because the malaria subspecies or strains used in the experiments (P. chabaudi chabaudi AS or CB strain and P. chabaudi adami) have usually been avirulent, though dependent upon the mouse strain, when transferred to immunocompetent hosts but virulent to immunocompromised hosts. As shown in Figs.…”

Section: Discussionsupporting

confidence: 84%

Analysis of roles of natural killer cells in defense against Plasmodium chabaudi in mice

Kitaguchi

Nagoya

Amano

et al. 1996

Parasitology Research

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Mice that have recovered from a primary infection with Plasmodium chabaudi have been shown to resist a secondary infection. In the present study the authors investigated how natural killer (NK) cells were involved in this resistance. Spleen cells from P. chabaudiprimed C57BL/6 mice could transfer protection against P. chabaudi infection into naive syngeneic mice, but spleen cells from unprimed mice could not. T-enriched cells purified from primed spleen cells could also transfer such protection. Transfer of NK cells from primed spleen cells failed to protect against challenge infection. However, depletion of NK cells in host mice by injection of an anti-NK1.1 monoclonal antibody resulted in higher mortality relative to controls. The possible protective roles of NK cells in P. chabaudi infection are discussed.

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Section: Discussionsupporting

confidence: 84%

Analysis of roles of natural killer cells in defense against Plasmodium chabaudi in mice

Kitaguchi

Nagoya

Amano

et al. 1996

Parasitology Research

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“…However, since these mice were also depleted of Thy-i+ cells in all lymphoid organs examined and were functionally severely impaired in their immune responsiveness, this is unlikely. The courses of infection in the double-depleted mice and the CD4-depleted mice were similar to those observed for P. chabaudi adami in nude mice (2,3,21) and P. chabaudi chabaudi AS in adult thymectomized, irradiated mice (33,34) in that the parasites are not cleared but the majority of mice remain alive for at least 30 to 65 days. The low transient malaria-specific IgM antibodies may play a role in this.…”

Section: Discussionsupporting

confidence: 70%

Roles of CD4- and CD8-bearing T lymphocytes in the immune response to the erythrocytic stages of Plasmodium chabaudi

et al. 1988

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The possible role of CD4and CD8-bearing T lymphocytes in parasite clearance in vivo was investigated, using Plasmodium chabaudi in C57BL/6 mice as a model. Monoclonal antibodies specffic for the CD4 and CD8 molecules were administered in vivo to deplete selectively the appropriate subset of T cells. The efficacy of depletion was ascertained by flow cytometry and functional studies. These mice were then infected with P. chabaudi, and the course of infection was followed. The control groups had maximum parasitemias of approximately 30% 10 days after infection, and the infection was cleared within 27 days. Mice without CD4+ cells had significantly higher parasitemias which they were unable to reduce below 20% for the duration of the experiment. Mice without CD8 cells had slightly higher parasitemias which were cleared after 34 days. Because of the possibility that CD8+ cells alone could not be activated in the absence of growth factors, exogenous interleukin-2 was administered to the mice depleted of CD4 cells. This did not significantly affect parasitemias, and the mice were still unable to clear their infections. The data suggest that CD4+ T cells play a crucial role in the protective immune response to the erythrocytic stages of P. chabaudi.

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“…Acquired immunity to the asexual blood stages of malaria in mice involves both cell-mediated and antibody-dependent mechanisms through activation of 2 subsets of CD4 + T cells, i.e., Th1 and Th2 cells [4]. In a self resolving P. chabaudi AS infection, there was a sequential Th1/Th2 response in which the Th1 subset responsible to control the acute phase of the patent para-sitemia while Th2 response rises when infection becomes chronic [5,6] and could play a role in the elimination of the infection [7]. In a virulent infection of P. yoelii, a failure to induce adequate activation of both Th1 and Th2 subsets resulted in a fatal outcome [8].…”

Section: Introductionmentioning

confidence: 99%

Immune Responses of NIH Mice Infected with Avirulent and Virulent Strains of Plasmodium chabaudi adami Single and Mixed Infections

Namazi

Phillips

2010

Korean J Parasitol

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An understanding of the nature of the immune response to asexual erythrocytic stages of malaria parasites will facilitate vaccine development by identifying which responses the vaccine should preferentially induce. The present study examined and compared the immune responses of NIH mice in either single or mixed infections with avirulent (DK) or virulent (DS) strains of Plasmodium chabaudi adami using the ELISA test for detecting and measurement of cytokines and antibody production. In both single and mixed infections, the study showed that both cell- and antibody-mediated responses were activated. In all experiments, an early relatively high level of IFN-gamma and IgG2a during the acute phase of the infection, and later elevation of IL-4 and IgG1, suggested that there was a sequential Th1/Th2 response. However, in the avirulent DK strain infection a stronger Th1 response was observed compared to the virulent DS strain-infection or in mixed infections. In the virulent DS infection, there was a stronger Th2 response compared to that in the DK and mixed infections. The faster proliferation rate of the virulent DS strain compared to the DK strain was also evident.

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Plasmodium chabaudi: Adoptive transfer of immunity with different spleen cell populations and development of protective activity in the serum of lethally irradiated recipient mice

Cited by 9 publications

References 19 publications

Analysis of roles of natural killer cells in defense against Plasmodium chabaudi in mice

Analysis of roles of natural killer cells in defense against Plasmodium chabaudi in mice

Roles of CD4- and CD8-bearing T lymphocytes in the immune response to the erythrocytic stages of Plasmodium chabaudi

Immune Responses of NIH Mice Infected with Avirulent and Virulent Strains of Plasmodium chabaudi adami Single and Mixed Infections

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