Plasmin degradation of fibrin coatings on synthetic polymer substrates

Abstract

The goal of this research was to evaluate the in vitro stability of fibrin coatings on polymeric materials in the presence of plasmin. Factor XIIIa-crosslinked and noncrosslinked fibrin layers were coated on three different polyurethane substrates: Corethane, Tegaderm, and a biodegradable polyurethane, PCL/HDI/Phe. Degradation assays indicated that crosslinking the fibrin coatings enhanced the stability of the coatings on both Tegaderm and PCL/HDI/Phe; however, the persistence of the coating on the woven Corethane was not influenced by crosslinking. Degradation assay results also showed that the fibrin coating on the Corethane was significantly less stable than the fibrin coatings on the Tegaderm and PCL/HDI/Phe films. The chromogenic substrate assay data showed crosslinking did not affect the specific plasmin activity on the coatings; therefore, the increased stability resulting from crosslinking was not achieved through a reduction of fibrinolysis. The plasmin activity on the coated Corethane samples was much greater than that on either of the coated flat wound dressing materials. The large surface area of Corethane, a porous woven vascular graft material, may have had a direct influence on the fibrinolysis of its coatings by providing a large number of tissue-type plasminogen activator (tPA) binding sites. A thin, crosslinked, fibrin-coated polyurethane provides a theoretically attractive biomaterial for use in a wound dressing application and should be subject to ongoing research.

Form Follows Function: Advances in Trilayered Structure Replication for Aortic Heart Valve Tissue Engineering

“…Fibrin is a biodegradable, biocompatible matrix protein involved in wound healing and can be produced from a patient’s blood and therefore does not initiate inflammation, tissue necrosis, or fibrosis [79, 89–91]. Jockenhoevel et al used molded fibrin gels as a potential scaffold for TE [79].…”

Endothelialized and preconditioned natural umbilical arteries with long term patency open the route for future human uses

“…Before exposure to shear stress, the distribution of ECs was comparable for the uncoated or (PAH-PSS) 3 -PAH coated surfaces. After exposure to shear stress, the retention was quite larger for (PAH-PSS) 3 Several authors showed that exposure to low mechanical forces like shear stress orients cells toward flow direction and then improves ECs adhesion and retention in vivo [2,14,24,36,39,40,57]. Therefore, we tested the hypothesis that an exposure of the endothelialized PEMs coated graft to a preconditioning shear stress of 1 Pa for three hours before graft implantation could improve cell retention after implantation.…”

“…Compared to fibrin, a PSS/PAH film could resist quite longer to enzymatic degradations. Moreover, PEM films are also extremely thin and very easy to deposit inside small caliber vessels (<6 mm) in a reproducible way [3,15]. In a next step, we checked, in vivo, the patency of the endothelialized and coated grafts.…”

“…3A). 3 -PAH film, endothelialized and finally exposed to shear stress. The adhesion and distribution of ECs were checked thanks to the labeling of two endothelial markers: CD31 and von willebrand (vWF).…”

Preparation and characterization of fibrin-chitosan composite and its in vivo studies

“…Similarly, degradation and pitting of the film is attributed to release of reactive oxygen intermediate at the FC-cell interface. Platelet aggregation on fibrin surfaces has been reported (Skarja et al 1998) and degradation of fibrin proceeds by the action of plasmin (Bence et al 1999). Complete degradation of FC film was observed on 30 PID and hence it is concluded that it is biocompatible, biodegradable and can serve as a potential wound dressing material.…”