Plasmids of Yersinia enterocolitica and Yersinia pseudotuberculosis: Analysis with restriction endonucleases

Wartenberg, Klaus; Weninger, Jürgen; Röllinghoff, Martin

doi:10.1016/s0176-6724(88)80005-1

Cited by 3 publications

(4 citation statements)

References 23 publications

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“…Y. enterocolitica strains and growth conditions. Y. enterocolitica serotype 03 with a 47-megadalton plasmid (p+) (25) was isolated from an infected dog (19). Y. enterocolitica serotype 03 p-refers to the same strain after plasmid curing.…”

Section: Methodsmentioning

confidence: 99%

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Influence of surface modulations by enzymes and monoclonal antibodies on alternative complement pathway activation by Yersinia enterocolitica

Wächter

Brade

1989

Infect Immun

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Effector mechanisms resulting from alternative complement pathway (ACP) activation cannot act efficiently against Yersinia enterocolitica serotype 03, as indicated by poor C3 to C9 consumption and by survival in EGTA (ethyleneglycoldiaminetetraacetic acid) Mg-serum. These results were not influenced by the lack or presence of plasmid-encoded outer membrane proteins or lipopolysaccharides (LPS) with different amounts of side chains or by treatment of the bacteria with pronase or neuraminidase. Surface modulation of Y. enterocolitica with polyclonal immunoglobulin G or the immunoglobulin G fragments F(ab')2 and Fab always converted Y. enterocolitica to a high ACP activator, with strong C3 to C9 consumption and surface deposition of activated C3. Killing of Y. enterocolitica as a result of antibody-mediated ACP activation was observed only with bacteria grown at 22°C but not with bacteria from 37°C cultures. The expression of complement resistance in Y. enterocolitica grown at 37°C was not influenced by the presence or absence of plasmids. Using different monoclonal antibodies (MAb), we found that MAb with LPS specificity mediated ACP activation, whereas MAb specific for different plasmid-encoded outer membrane proteins were ineffective, despite surface binding. These results suggest a major inhibitory role of LPS on ACP activation which was neutralized by LPS-specific antibodies.

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Section: Methodsmentioning

confidence: 99%

“…Y. enterocolitica serotype 09 p+ and p-refer to the plasmidpositive and plasmidless strains, respectively. The absence or presence of plasmids was verified by agarose gel electrophoresis (25). All strains were maintained on blood agar plates at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Influence of surface modulations by enzymes and monoclonal antibodies on alternative complement pathway activation by Yersinia enterocolitica

Wächter

Brade

1989

Infect Immun

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“…ELISA-technique, isolation of plasmids and digestion by the restriction endonucleases EcoRI, BamHI and HindlII, as well as agarose gel electrophoresis and immunoblotting were performed as previously described in detail [22,23].…”

Section: Production Of Monoclonal Antibodies (Mab)mentioning

confidence: 99%

“…The plasmidencoded OMPs expressed by Y. enterocolitica are similar in size when compared with those expressed by Y. pseudotuberculosis [ 1,19]. However size and restriction enzyme pattern of the OMP-encoding plasmids vary to some extent among different serotypes although an overall similarity has become apparent [3,6,17,21,22].…”

Section: Introductionmentioning

confidence: 99%

Detection of cross-reacting epitopes on plasmid-encoded outer membrane proteins of enteropathogenic Yersinia by monoclonal antibodies

Weninger

Schoerner

Wartenberg

et al. 1989

Med Microbiol Immunol

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Plasmid-positive Yersinia bacteria of different species and different serotypes were analysed with respect to the immunological relationship of two of their plasmid-encoded outer membrane proteins (OMPs) by the immunoblot technique using three monoclonal antibodies (mAb), which were induced against OMPs of Yersinia enterocolitica serotype O:9 bacteria. Evidence is presented that these OMPs display discrete epitopes, which are common to all plasmid-positive Yersinia strains tested, with only one exception, indicating a close structural relationship of the OMPs analysed.

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Plasmids and outer membrane proteins of Yersinia enterocolitica and related species of swine origin

Kwaga

Iversen

1993

Veterinary Microbiology

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Plasmids of Yersinia enterocolitica and Yersinia pseudotuberculosis: Analysis with restriction endonucleases

Cited by 3 publications

References 23 publications

Influence of surface modulations by enzymes and monoclonal antibodies on alternative complement pathway activation by Yersinia enterocolitica

Influence of surface modulations by enzymes and monoclonal antibodies on alternative complement pathway activation by Yersinia enterocolitica

Detection of cross-reacting epitopes on plasmid-encoded outer membrane proteins of enteropathogenic Yersinia by monoclonal antibodies

Plasmids and outer membrane proteins of Yersinia enterocolitica and related species of swine origin

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