Plasmid Replicons and β-Lactamase-Encoding Genes of Multidrug-Resistant <i>Escherichia coli</i> Isolated from Humans and Food Animals in Lagos, Southwest Nigeria

Adenipekun, E.; Jackson, Charlene R.; Ramadan, Hazem; Iwalokun, BA; Frye, Jonathan G.; Barrett, John B.; Hiott, Lari M.; Woodley, Tiffanie A.; House, Sandra L.; McMillan, Elizabeth A.; Sharma, Poonam; Oluwadun, Afolabi

doi:10.1089/mdr.2018.0305

Cited by 14 publications

(10 citation statements)

References 60 publications

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“…Our findings showed that plasmid incompatibility (Inc) types, IncFIB (12/13, 92.3%) and IncFII (11/13, 84.6%) were the predominant plasmid replicons identified from retail food and human E. coli ( Table 1 ). This was in agreement with previous studies that determined higher frequencies of IncF replicon types among E. coli isolates, especially those that were MDR ( Carattoli, 2009 ; Yang et al., 2015 ; Adenipekun et al., 2019 ). A recent study from Egypt demonstrated the existence of IncF replicon types in NDM-producing E. coli isolates from humans and dogs ( Ramadan et al., 2020a ).…”

Section: Resultssupporting

confidence: 93%

“…The wide dissemination of IncF replicons among E. coli isolates from different sources, and the association of resistance traits, e.g. genes encoding extended spectrum b-lactamases (ESBLs), carbapenemases, aminoglycoside-modifying enzymes, and PMQR genes, to this replicon type, could be responsible for possible interspecies dissemination of these plasmid-mediated resistance genes between humans and animals (Rozwandowicz et al, 2018;Adenipekun et al, 2019). fold metalloprotein), whereas bla CTX-M-15 in human isolate 20ST was located upstream to ISEcp1 (Figures 1A, B).…”

Section: Resultsmentioning

confidence: 99%

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Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Ramadan

Soliman

Hiott

et al. 2021

Front. Cell. Infect. Microbiol.

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In this study, multidrug-resistant (MDR) Escherichia coli isolates from retail food and humans assigned into similar Multilocus Sequence Types (MLST) were analyzed using whole genome sequencing (WGS). In silico analysis of assembled sequences revealed the existence of multiple resistance genes among the examined E. coli isolates. Of the six CTX-M-producing isolates from retail food, blaCTX-M-14 was the prevalent variant identified (83.3%, 5/6). Two plasmid-mediated fosfomycin resistance genes, fosA3, and fosA4, were detected from retail food isolates (one each from chicken and beef), where fosA4 was identified in the chicken isolate 82CH that also carried the colistin resistance gene mcr-1. The blaCTX-M-14 and fosA genes in retail food isolates were located adjacent to insertion sequences ISEcp1 and IS26, respectively. Sequence analysis of the reconstructed mcr-1 plasmid (p82CH) showed 96–97% identity to mcr-1-carrying IncI2 plasmids previously identified in human and food E. coli isolates from Egypt. Hierarchical clustering of core genome MLST (HierCC) revealed clustering of chicken isolate 82CH, co-harboring mcr-1 and fosA4 genes, with a chicken E. coli isolate from China at the HC200 level (≤200 core genome allelic differences). As E. coli co-harboring mcr-1 and fosA4 genes has only been recently reported, this study shows rapid spread of this genotype that shares similar genetic structures with regional and international E. coli lineages originating from both humans and food animals. Adopting WGS-based surveillance system is warranted to facilitate monitoring the international spread of MDR pathogens.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Ramadan

Soliman

Hiott

et al. 2021

Front. Cell. Infect. Microbiol.

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“…There is a need to monitor the potential sources of human infections using high discriminatory molecular typing methods. This is especially true considering the increase in antimicrobial resistance and the possibility of dissemination of resistance traits, especially those mediated by MGE through the food chain to humans [11,18,19]. In Egypt, the available information about the clonality of antimicrobial resistant E. coli from humans and food-producing animals is still minimal.…”

Section: Discussionmentioning

confidence: 99%

“…PCR with whole cell templates of all 120 E. coli isolates was used to screen for the presence of genes encoding resistance to β-lactams (bla CTX , bla TEM , bla CMY , bla SHV , bla OXA ), chloramphenicol (cat1, cat2, floR), tetracycline (tetA, tetB), sulfisoxazole (sul1, sul2), streptomycin (strA, strB), trimethoprim/ sulfamethoxazole (dhfr1, dhfr5, dhfr12, dhfr13), and azithromycin (mphA) as previously described [19,44,45]. PCR assay for each resistance gene was performed in 25 µL reaction mixture containing 2.5 µL of…”

Section: Resistance Genotypes and E Coli Phylogroupingmentioning

confidence: 99%

Antimicrobial Resistance, Genetic Diversity and Multilocus Sequence Typing of Escherichia coli from Humans, Retail Chicken and Ground Beef in Egypt

et al. 2020

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Contamination of retail foods with foodborne pathogens, particularly the antimicrobial resistant ones, poses a persistent threat to human health. There is a dearth of information about the overlapping Escherichia coli (E. coli) lineages circulating among retail foods and humans in Egypt. This study aimed to determine the clonal diversity of 120 E. coli isolates from diarrheic patients (n = 32), retail chicken carcasses (n = 61) and ground beef (n = 27) from Mansoura, Egypt using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Simpson’s index of diversity was calculated to compare the results of both typing methods. Antimicrobial resistance phenotypes, genotypes and phylogrouping of the isolates were also determined. Higher frequencies of antimicrobial resistance were found among chicken isolates compared to beef and human isolates; regardless of isolate source, the predominant antimicrobial resistances were found against ampicillin (87/120, 72.5%), tetracycline and sulfisoxazole (82/120, 68.3%, each), and streptomycin (79/120, 65.8%). None of the isolates displayed resistance to meropenem. The prevalent genes detected were tetA (64.2%), blaTEM (62.5%), sul1 (56.7%), floR (53.3%), sul2 (50%), strB (48.3%) and strA (47.5%) corresponding with resistance phenotypes. Alarmingly, blaCTX was detected in 63.9% (39/61) of chicken isolates. The majority of E. coli isolates from humans (90.6%), beef (81.5%) and chicken (70.5%) belonged to commensal phylogroups (A, B1, C). Using PFGE analysis, 16 out of 24 clusters (66.7%) contained isolates from different sources at a similarity level ≥75%. MLST results assigned E. coli isolates into 25, 19 and 13 sequence types (STs) from chicken, human and beef isolates, respectively. Six shared STs were identified including ST1011, ST156, ST48, ST224 (chicken and beef), ST10 (human and chicken) and ST226 (human and beef). Simpson’s index of diversity was higher for MLST (0.98) than PFGE (0.94). In conclusion, the existence of common genetic determinants among isolates from retail foods and humans in Egypt as well as the circulation of shared STs indicates a possible epidemiological link with potential zoonotic hazards.

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“…Seventeen (17) genes were detected from animals, 28 genes from humans and 12 genes from the environment. These genes belongs to the AmpC [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] , extended-spectrum [10][11][12][13][14][15]17,19,20, , narrow-spectrum [13][14][15][16]19,24,32,35,37,43,[57][58][59] , and carbapenemase beta-lactamase resistance genes 19,22,[59][60][61][62][63][64][65][66] . One health distribution of beta-lactamase resistance genes between the animal, human and environmental settings was presented in Table 1.…”

Section: One Health Distribution Of Beta-lactamase Resistance Genes Imentioning

confidence: 99%

Beta-Lactamase Resistance Genes in <em>Enterobacteriaceae</em> from Nigeria

Awosile¹,

Agbaje²,

Adebowale³

et al. 2020

Preprint

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This review was carried out to identify different beta-lactamase resistance genes reported in published literature from Nigeria and to determine the proportion estimates of the important beta-lactamase resistance genes in Nigeria. Sixty-three (63) articles were included in this review based on the eligibility criteria. All the beta-lactamases reported were detected from the Gram-negative bacteria, most especially from Enterobacteriaceae (n=53). Thirty-six different beta-lactamase genes have been reported from Nigeria. These genes belong to the narrow-spectrum, AmpC, extended-spectrum, and carbapenemase beta-lactamase resistance genes. Eight (8) genes (blaDHA, blaCTXM-1, blaCTXM-14, blaGES-1, blaVEB-1, blaOXA-1, blaOXA-2, and blaTEM-1) were shared between animals and humans, 5 genes (blaSHV-1, blaSHV-2, blaSHV-11, blaSHV-12, and blaNDM-1) were common to both humans and environment while none of the genes was unique to both animals and environment. Four genes including blaCMY, blaTEM-1, blaAmpC, and internationally pandemic blaCTXM-15 gene were unique to animals, humans, and the environment. No carbapenemase gene was reported from animals yet. The pooled proportion estimate of ESBL genes in Nigeria was 31% (95% CI: 26-36%, P<0.0001), while the estimate of blaCTXM-15 gene in Nigeria was 46% (95% CI: 36-57%, P<0.0001). The proportion estimate of AmpC genes was 32% (95% CI: 11-52%, P<0.001), while the estimate for carbapenemases was 8% (95% CI: 5-12%, P<0.001). This study has provided information on the beta-lactamases distribution in Nigeria. This is necessary for a better understanding of molecular epidemiology of clinically important beta-lactamases especially the extended-spectrum beta-lactamases and carbapenemases in Nigeria.

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Plasmid Replicons and β-Lactamase-Encoding Genes of Multidrug-Resistant Escherichia coli Isolated from Humans and Food Animals in Lagos, Southwest Nigeria

Cited by 14 publications

References 60 publications

Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Antimicrobial Resistance, Genetic Diversity and Multilocus Sequence Typing of Escherichia coli from Humans, Retail Chicken and Ground Beef in Egypt

Beta-Lactamase Resistance Genes in <em>Enterobacteriaceae</em> from Nigeria

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