Plasmid profiles, restriction fragment length polymorphisms and O-serotypes among<i>Vibrio anguillarum</i>isolates

Pedersen, Karl; Tiainen, T.; Larsen, J. L.

doi:10.1017/s0950268800059136

Cited by 20 publications

(16 citation statements)

References 36 publications

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“…However, the pJM1 and pColV-K30 plasmid-mediated iron uptake systems are unrelated (17,49). pJM1-like-plasmids, usually around 65 to 67 kbp, have been reported by us and others in different virulent V. anguillarum strains isolated from many epizootics throughout the world (30,32,46). In this family of plasmids the best characterized is the 65-kb pJM1 plasmid that has been isolated from the V. anguillarum strain 775 and is the one that we chose to sequence.…”

mentioning

confidence: 67%

“…Previous work has also shown microheterogeneity in many other examples of pJM1-like plasmids (30,32,42,43). For instance the pJM1-like plasmid pJHC1 from strain 531A shows two major changes, two extra insertions of the RS1 sequence and also an angR gene that has a single nucleotide change that results in an amino acid substitution in the AngR protein (H267N).…”

Section: Discussionmentioning

confidence: 95%

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Complete Sequence of Virulence Plasmid pJM1 from the Marine Fish PathogenVibrio anguillarumStrain 775

Lorenzo¹,

Stork²,

Tolmasky

et al. 2003

J Bacteriol

117

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The virulence plasmid pJM1 enables the fish pathogen Vibrio anguillarum, a gram-negative polarly flagellated comma-shaped rod bacterium, to cause a highly fatal hemorrhagic septicemic disease in salmonids and other fishes, leading to epizootics throughout the world. The pJM1 plasmid 65,009-nucleotide sequence, with an overall G؉C content of 42.6%, revealed genes and open reading frames (ORFs) encoding iron transporters, nonribosomal peptide enzymes, and other proteins essential for the biosynthesis of the siderophore anguibactin. Of the 59 ORFs, approximately 32% were related to iron metabolic functions. The plasmid pJM1 confers on V. anguillarum the ability to take up ferric iron as a complex with anguibactin from a medium in which iron is chelated by transferrin, ethylenediamine-di(o-hydroxyphenyl-acetic acid), or other iron-chelating compounds. The fatDCBA-angRT operon as well as other downstream biosynthetic genes is bracketed by the homologous ISV-A1 and ISV-A2 insertion sequences. Other clusters on the plasmid also show an insertion element-flanked organization, including ORFs homologous to genes involved in the biosynthesis of 2,3-dihydroxybenzoic acid. Homologues of replication and partition genes are also identified on pJM1 adjacent to this region. ORFs with no known function represent approximately 30% of the pJM1 sequence. The insertion sequence elements in the composite transposon-like structures, corroborated by the G؉C content of the pJM1 sequence, suggest a modular composition of plasmid pJM1, biased towards acquisition of modules containing genes related to iron metabolic functions. We also show that there is considerable microheterogeneity in pJM1-like plasmids from virulent strains of V. anguillarum isolated from different geographical sources.The fish pathogen Vibrio anguillarum strain 775 is the causative agent of vibriosis, a highly fatal hemorrhagic septicemic disease (3). This bacterium disseminates in the vertebrate host by using the otherwise unavailable iron bound by high-affinity iron binding proteins, such as transferrin and lactoferrin. Furthermore, V. anguillarum 775 has the ability to grow in vitro in media in which iron is chelated by transferrin, ethylenediamine-di(o-hydroxyphenyl-acetic acid), and other iron chelators (15, 40). The metabolic pathway supporting the ability of this bacterium to grow under iron-limiting conditions is linked to the presence in the bacterial cells of the virulence plasmid pJM1 (15).Iron metabolic plasmids are rare; in addition to the pJM1-like plasmids only the pColV-K30 family of plasmids, identified in human clinical strains of Escherichia coli and other enteric bacteria, have been associated with iron metabolism. However, the pJM1 and pColV-K30 plasmid-mediated iron uptake systems are unrelated (17, 49). pJM1-like-plasmids, usually around 65 to 67 kbp, have been reported by us and others in different virulent V. anguillarum strains isolated from many epizootics throughout the world (30,32,46). In this family of plasmids the best characterized is t...

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mentioning

confidence: 67%

Section: Discussionmentioning

confidence: 95%

Complete Sequence of Virulence Plasmid pJM1 from the Marine Fish PathogenVibrio anguillarumStrain 775

Lorenzo¹,

Stork²,

Tolmasky

et al. 2003

J Bacteriol

117

View full text Add to dashboard Cite

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“…Although a number of different serotypes were isolated from the surface and intestinal content of the net pen fish, the virulence plasmid (67 kb) were not found in any of the isolates. The presence of the plasmid in V. anguillarum serotype 01 makes this particular organism extremely infective Pedersen et al, 1996). If the virulent form of V. anguillurum serotype 01 had occurred a serious outbreak of vibriosis might have caused a much stronger difference between groups.…”

Section: Discussionmentioning

confidence: 99%

“…Buchmann et al/Aquaculture 156 (19971335-348 341 Colonies on TCBS, suspected as V. anguillarum, were subcultured and identified as described by Larsen (1990) and Aalbak and Pedersen (1992). Serotyping and plasmid profiling (Pedersen et al, 1996) were then conducted as described above to characterize this species.…”

Section: Fishmentioning

confidence: 99%

Vaccination improves survival of Baltic salmon (Salmo solar) smolts in delayed release sea ranching (net-pen period)

Buchmann¹,

Dalsgaard²,

Nielsen³

et al. 1997

Aquaculture

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, J. L. (1997). Vaccination improves survival of Baltic salmon ( Salmo salar ) smolts in delayed release sea ranching (net-pen period). Aquaculture,, 335-348. DOI: 10.1016/S0044-8486(97) AbstractBaltic salmon (Salmo s&r) of the Finnish Iijoki stock were hatched and reared in freshwater in a salmon hatchery on the Danish island of Bornholm in the Baltic sea. Salmon parr were divided in three groups each comprising 22 000 fish. One group was vaccinated by intraperitoneal injection with a non-mineral oil-adjuvanted vaccine consisting of formalin killed Aeromonas salmonicida, Yersinia ruckeri and Vibrio anguillarum (serotype 0 1 and 02). A second group was vaccinated by 1 h bathing in a corresponding vaccine without adjuvant. A third group was left as untreated control. Subsequently, presmolt groups were transferred to three identical net-pens located next to each other in the Baltic Sea (salinity 8 ppt), 500 m from the north-eastern coast of Bornholm, where they were studied for four months until tagging and release for restocking purposes. Mortality during this period in the ip vaccinated group was minimal (0.02%, RPS (relative per cent of survival) 99.80) and significantly lower compared to 10.13% mortality in the control group and 2.51% mortality (RPS 75.2) in the bath vaccinated group. Specific disease outbreaks were not observed during the four months. Growth was significantly enhanced in the injection vaccinated group compared to both the unvaccinated control and the bath vaccinated group. The humoral antibody response to the various bacteria was significantly elevated in the injection vaccinated group showing 4-5 fold titre increases three and four months after immunization. In contrast, no increase of titres was seen in the bath vaccinated and untreated groups. Buchmann et al./Aquaculture 156 (1997) 335-348 Marked cellular reactions in the abdominal cavity of injected fish were registered. A total of 3000 fish have been tagged and released to evaluate the effect of vaccination on the recapture rate. The implications of immunoprophylactic measures in the restocking programme with Baltic salmon are discussed. 0 1997 Elsevier Science B.V.

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“…Even though no visible correlation was observed between antibiotic resistance pattern and presence of plasmids in the study, further plasmid curing experiments need to be performed to confirm the same. The plasmids profiles in vibrios have been previously studied in some species such as V. parahaemolyticus 30 ; V. ordalii 31 , V. vulnificus 32 and V. salmonicida 33 , and most extensively in V. anguillarum, where a high diversity of profiles was observed 15,34 . Presence of plasmids in Vibrio species of both polluted and pristine environments may be ecologically important to the survival of these bacteria in the environment 28 .…”

Section: Samples From Two Different Estuaries (Cochin Andmentioning

confidence: 99%

Prevalence and Multiple Antibiotic Resistance of <i>Vibrio coralliilyticus</i>, along the Southwest Coast of India

Silvester¹,

Alexander²,

George³

et al. 2017

Current Science

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Plasmid profiles, restriction fragment length polymorphisms and O-serotypes amongVibrio anguillarumisolates

Cited by 20 publications

References 36 publications

Complete Sequence of Virulence Plasmid pJM1 from the Marine Fish PathogenVibrio anguillarumStrain 775

Complete Sequence of Virulence Plasmid pJM1 from the Marine Fish PathogenVibrio anguillarumStrain 775

Vaccination improves survival of Baltic salmon (Salmo solar) smolts in delayed release sea ranching (net-pen period)

Prevalence and Multiple Antibiotic Resistance of <i>Vibrio coralliilyticus</i>, along the Southwest Coast of India

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