Plasmid profile analysis in identification of epidemic strains of Salmonella enterica serovar Enteritidis

Abstract

The strains of S. Enteritidis, isolated in outbreaks of enterocolitis as well as from spordic cases of diarrhea in the same period of time and at the same area, frequently exhibit the same plasmid profile characterized by a single plasmid of 38 MDa. Therefore, in most cases plasmid profile analysis is not valuable in the identification of epidemic strains of S. Enteritidis. However, for this purpose plasmid profile analysis could be used when drug-resistant strains of S. Enteritidis are isolated, as they often possess additional resistant plasmids what increases discrimination power of this method.

Differentiation ofSalmonellaEnteritidis Isolates by Fluorescent Amplified Fragment Length Polymorphism

“…Several molecular epidemiological techniques, such as pulsed-field gel electrophoresis (PFGE) (Ridley et al, 1998;Fernandes et al, 2003), plasmid profiling (Olsen et al, 1994;Miljkovic-Selimovic et al, 2008;Kalender et al, 2009), random amplified polymorphic DNA (RAPD) (Betancor et al, 2004), amplified fragment length polymorphism (AFLP) (Desai et al, 2001;Scott et al, 2001), and multilocus sequence typing (Kotetishvili et al, 2002;Fakhr et al, 2005), have been performed together with phenotypic techniques in an attempt to improve the reproducibility and discriminatory ability of Salmonella Enteritidis genetic typing. The implementation of these techniques has led to an enhanced detection of outbreaks worldwide (Winocur, 2003;Sukhnanand et al, 2005).…”