Plasmid pDGO100 Contains a Second Integron with the Trimethoprim Resistance GenedfrA7as the Inserted Cassette

Burnside, Joan; Obbink, D.J. Groot

doi:10.1006/plas.1996.0008

Cited by 14 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, restriction digests indicated that the plasmid backbones of pRMH760 and pDGO100 are different (data not shown), and previous studies have shown that pDGO100 contains two class 1 integrons, with the dfrA7 cassette in the second one (8). Although the part of the tni module found in In34 is present in pDGO100, it did not include Tn4352, indicating that this transposon was acquired more recently by In34.…”

Section: Vol 47 2003 In5 Family Class 1 Integron Containing Orf513 343mentioning

confidence: 74%

In34, a Complex In5 Family Class 1 Integron Containing orf513 and dfrA10

Partridge

Hall

2003

Antimicrob Agents Chemother

130

154

View full text Add to dashboard Cite

A complex class 1 integron, In34, found in a conjugative plasmid from a multidrug-resistant Klebsiella pneumoniae strain isolated in 1997 at a hospital in Sydney, Australia, was shown to have a backbone related to that of In2, which belongs to the In5 family. In In34, the aadB gene cassette replaces the aadA1a cassette in In2, and two additional resistance genes, dfrA10 and aphA1, that are not part of a gene cassette are present. The aphA1 gene is in a Tn4352-like transposon that is located in the tniA gene. The dfrA10 gene lies adjacent to a 2,154-bp DNA segment, known as the common region, that contains an open reading frame predicting a product of 513 amino acids (Orf513). Orf513 is 66 and 55% identical to the products of two further open reading frames that, like the common region, are found adjacent to antibiotic resistance genes. A 27-bp conserved sequence was found at one end of each type of common region. The loss of dfrA10 due to homologous recombination between flanking direct repeats and incorporation of the excised circle by homologous recombination were demonstrated. Part of In34 is identical to the sequenced portion of In7, which is from a multidrug-resistant Escherichia coli strain that had been isolated 19 years earlier in the same hospital. In34 and In7 are in plasmids that contain the same six resistance genes conferring resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, neomycin, tobramycin, trimethoprim, and sulfonamides, but the plasmid backbones appear to be unrelated, suggesting that translocation of a multiple-drug-resistance-determining region as well as horizontal transfer may have occurred.

show abstract

Section: Vol 47 2003 In5 Family Class 1 Integron Containing Orf513 343mentioning

confidence: 74%

In34, a Complex In5 Family Class 1 Integron Containing orf513 and dfrA10

Partridge

Hall

2003

Antimicrob Agents Chemother

130

154

View full text Add to dashboard Cite

show abstract

“…Many different genes responsible for antibiotic resistance have been found in integrons, among which those coding for different aminoglycosidemodifying enzymes, b-lactamases or dihydrofolate reductases can be found [2,8,18,19,27]. Moreover, some different ORF with unknown functions have been described [8,20,27,28].…”

Section: Discussionmentioning

confidence: 99%

“…Two conserved regions: an integrase encoding gene in the 5' segment (5'CS) and the genes qacE1 , sulI and the orf5 in the 3' segment (3'CS). Between these conserved regions a variable region is found in which gene cassettes are inserted [2]. Up to three different gene cassettes are commonly found inserted in one integron.…”

Section: Introductionmentioning

confidence: 99%

Integron-mediated antibiotic multiresistance in Acinetobacter baumannii clinical isolates from Spain

Ruiz

Navia

Casals

et al. 2003

Clinical Microbiology and Infection

View full text Add to dashboard Cite

show abstract

“…The sequences of the integrase (intI), the common promoter (P ant ) (5), the attachment site (attI), and the trimethoprim resistance gene dfr7 (GenBank accession no. X58425) (4,21) were located upstream from aadA5. In Danish veterinary practice, the combination of trimethoprim and sulfonamides is widely utilized to treat infectious diseases.…”

mentioning

confidence: 99%

Novel Streptomycin and Spectinomycin Resistance Gene as a Gene Cassette within a Class 1 Integron Isolated from Escherichia coli

Sandvang¹

1999

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present study has revealed the sequence of a new gene, aadA5, integrated as a gene cassette together with the trimethoprim resistance genedfr7 in a class 1 integron. The integron was located on a plasmid and was identified in a pathogenic porcineEscherichia coli isolate.

show abstract

Plasmid pDGO100 Contains a Second Integron with the Trimethoprim Resistance GenedfrA7as the Inserted Cassette

Cited by 14 publications

References 0 publications

In34, a Complex In5 Family Class 1 Integron Containing orf513 and dfrA10

In34, a Complex In5 Family Class 1 Integron Containing orf513 and dfrA10

Integron-mediated antibiotic multiresistance in Acinetobacter baumannii clinical isolates from Spain

Novel Streptomycin and Spectinomycin Resistance Gene as a Gene Cassette within a Class 1 Integron Isolated from Escherichia coli

Contact Info

Product

Resources

About