Plasmid-mediated quinolone resistance – PMQR MECHANIZMY OPORNOŚCI NA FLUOROCHINOLONY

Piekarska, Katarzyna

doi:10.21307/pm-2018.57.1.047

Cited by 2 publications

(2 citation statements)

References 93 publications

(178 reference statements)

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“…The Genes QnrB10 QnrB2 QnrS1 QnrS2, QnrA1, QnrB family and Mfd, were involved in target protein protection mechanism. QnrA, QnrB, and QnrS, qnr C and qnrD, qnr VC had been described in earlier studies as mediating quinolone resistance in mainly Gram-negative organsims 45 . However, in E coli, all except qnrVc have been detected at different prevalences in different regions 10,11,16,46 , using the multiplex PCR technique.…”

Section: Discussionmentioning

confidence: 92%

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Bioinformatics analysis of clinical isolates of Escherichia coli from Nigeria and other countries for quinolone and fluoroquinolone resistance

Udochi A. Ugo,

Ebele Onuigbo,

Iniekong Philip Udoh

et al. 2024

Magna Sci. Adv. Res. Rev.

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Quinolones were the drugs of choice for the treatment of bacterial infections, especially, infections caused by Gram negative bacteria. Unfortunately, these drugs have been resisted by the microbial agents including Escherichia coli, known to be the leading cause of Urinary Tract Infection (UTI) globally. This study therefore was aimed at detecting all the genes involved in Quinolone resistance by the E. coli pathogen isolated from Nigeria and from other geographical regions, using robust techniques. Twenty-three sequence data files of Escherichia coli from various countries of the world were retrieved from the National Centre for biotechnology Information (NCBI) database and sent for genome assembly for processing of the short reads into long reads. The outcome was config. fasta files which were comprehensively annotated and characterized for genetic functions and mechanisms. A total of 208 antibiotic resistance genes were detected, out of which 27(13.0%) were linked to quinolone resistance and 14(6.7%) to multi- drug resistance. The result of this study significantly implicated many genes in quinolone resistance; notably were the efflux pump genes and their high percentage abundance. We recommend in-depth study of the genes for their expression capabilities, also the structure and features of the efflux pump genes to enable proper redesigning of drugs by integrating anti efflux pump substances that will selectively prevent the expression of the genes for antibiotic resistance, without any harm to the host, or that can destabilize the positive regulation of the operon for antibiotic resistance.

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Section: Discussionmentioning

confidence: 92%

“…In this study qnr D and qnr C and qnr VC were not detected. The qnr genes code for proteins of the pentapeptide repeat family that protect the topoisomerase enzymes from the lethal effect of the quinolone antibiotic 45 . The frequency of the qnr genes was low in this study.…”

Section: Discussionmentioning

confidence: 99%