Plasmid-mediated quinolone resistance conferred by qnrS1 in Salmonella enterica serovar Virchow isolated from Turkish food of avian origin

Avsaroglu, M. Dilek; Helmuth, Reiner; Junker, Ernst; Hertwig, Stefan; Akçelik, Mustafa; Bozoğlu, Faruk; Guerra, Beatriz

doi:10.1093/jac/dkm352

Cited by 37 publications

(20 citation statements)

References 12 publications

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“…In another study from Turkey, Nazik et al (2009) reported qnr genes in Enterobacter spp., E. coli and K. pneumoniae. In agreement with previous studies (Kehrenberg et al 2006;Avsaroglu et al 2007), our results confirmed the presence of qnrS gene associated with fluoroquinolone resistance in two different Salmonella isolates from Turkey. In contrast, Yu et al (2011), reported that all Salmonella isolates were negative for qnr genes, whereas a high prevalence (37.1%) of aac(6')-Ib-cr was found.…”

Section: Discussionsupporting

confidence: 93%

Plasmid-mediated quinolone resistance in Salmonella serotypes isolated from chicken carcasses in Turkey

Ata¹,

Yibar

Arslan

et al. 2014

Acta Vet. Brno

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Quinolones have been extensively used for treatment of a variety of invasive and systemic infections of salmonellosis. Widespread use of these agents has been associated with the emergence and dissemination of quinolone-resistant pathogens. The quinolone resistance and plasmid-mediated quinolone resistance determinants (qnrA, qnrB, qnrS and aac(6')-Ib-cr) of 85 Salmonella isolates from chicken carcasses were investigated in this study. Isolates were serotyped according to the Kauffman-White-Le Minor scheme, and broth microdilution method was used to determine quinolone resistance. Plasmid-mediated quinolone resistance genes were investigated by real-time PCR and positive results were confirmed by sequencing. Among the Salmonella isolates, 30/85 (35%) and 18/85 (21%) were found to be resistant to enrofloxacin (MIC ≥ 2 mg/ml), and danofloxacin (MIC ≥ 2 mg/ml), respectively. All the isolates were negative for qnrA, qnrB and aac(6')-Ib-cr genes, nevertheless 2% (S. Brandenburg and S. Dabou) were positive for qnrS (qnrS1 determinant). This study is the first and unique investigating the plasmidmediated quinolone resistance determinants of Salmonella isolated from chicken carcasses in Turkey.

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Section: Discussionsupporting

confidence: 93%

Plasmid-mediated quinolone resistance in Salmonella serotypes isolated from chicken carcasses in Turkey

Ata¹,

Yibar

Arslan

et al. 2014

Acta Vet. Brno

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“…Because of the widespread usage of antimicrobial agents in both developed and developing countries, antibiotic resistance in some types of Salmonella infections is increasing. Increasing antimicrobial resistance in non-typhoid Salmonella species has been a serious problem for public health [11,12]. Antibiotic resistance profiles of food-originated DMC coded 13 S. Infantis isolates, determined in the present study are compatible with the previous findings [13][14][15].…”

Section: Resultssupporting

confidence: 89%

Effects of sub-MIC antibiotic concentrations on biofilm production of Salmonella Infantis

Tezel

Akçelik

Yüksel

et al. 2016

Biotechnology & Biotechnological Equipment

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In the present study, 13 Salmonella Infantis strains, which have been originated from Turkey, were selected due to their clinical and industrial relevance, sufficient biofilm producing capability and multidrug resistance. Although all tested strains were built up of thin pellicle, optimum pellicle formation has occurred at 28 C. All S. Infantis biofilms were categorized as 'bdar' morphotype following the incubation at both 20 and 28 C, while they were categorized as 'saw' morphotype at 37 C. Under a certain incubation temperature (28 C), 84.62% of strains have formed strong biofilm structures. By using the disk diffusion method, high levels of resistance have been observed among tested bacteria against nalidixic acid (100%), spectinomycin (100%), streptomycin (92.3%), tetracycline (92.3%), kanamycin (76.9%) and neomycin (76.9%). Further studies were performed with S. Infantis DMC 12 strain, due to its capability to produce biofilm and multidrug resistance phenotype. Gentamycin (>64 mg/mL, 2 £ MIC) and tetracycline (>128 mg/mL, 4 £ MIC) were determined as the most effective antibiotics against biofilm formation. The biofilm forms have showed increased antimicrobial resistance when it was compared to the planktonic bacteria. The highest resistance rates of the biofilm bacteria were observed to neomycin (12 £ MIC) followed by spectinomycin (10 £ MIC) and streptomycin (10 £ MIC). Biofilm structure was induced as a result of nalidixic acid, spectinomycin, tetracycline and neomycin treatment at sub-MIC concentrations of tested antibiotics.

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“…Resistance genes commonly present in Shigella spp. and Enterobacteriaceae, including the SRL, Tn7, gyrA, dfrA5, bla CTX-M , bla TEM , bla SHV , bla CMY , qnr, qepA, and aac(6=)-Ib-cr, were selected as references (9,(28)(29)(30)(31)) (see Table S1 in the supplemental material). The antibiotic resistance genes were searched using BLASTn, with an E value of 1 ϫ 10 Ϫ15 as the cutoff value for a significant match, based on the data generated by whole-genome sequencing.…”

Section: Methodsmentioning

confidence: 99%

Genomic Portrait of the Evolution and Epidemic Spread of a Recently Emerged Multidrug-Resistant Shigella flexneri Clone in China

et al. 2014

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Plasmid-mediated quinolone resistance conferred by qnrS1 in Salmonella enterica serovar Virchow isolated from Turkish food of avian origin

Abstract: This is the first study describing the presence of qnrS1 genes in bacterial isolates from Turkey. The pRQ2006 plasmid seems to be more related to the S. flexneri 2b qnrS1 plasmid pAH0376 than to the Salmonella qnrS1-carrying plasmids pINF5 and TPqnrS-2.

Cited by 37 publications

References 12 publications

Plasmid-mediated quinolone resistance in Salmonella serotypes isolated from chicken carcasses in Turkey

Plasmid-mediated quinolone resistance in Salmonella serotypes isolated from chicken carcasses in Turkey

Effects of sub-MIC antibiotic concentrations on biofilm production of Salmonella Infantis

Genomic Portrait of the Evolution and Epidemic Spread of a Recently Emerged Multidrug-Resistant Shigella flexneri Clone in China

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