Plasmid-Mediated High-Level Resistance to Aminoglycosides in
            <i>Enterobacteriaceae</i>
            Due to 16S rRNA Methylation

Galimand, Marc; Courvalin, Patrice; Lambert, Thierry

doi:10.1128/aac.47.8.2565-2571.2003

Cited by 312 publications

(233 citation statements)

References 47 publications

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“…Plasmids from ESBL-producing K. pneumoniae isolates were screened by PCR for the following b-lactamase genes -bla CTX-M , bla TEM , bla SHV , bla OXA-1 , bla KPC , bla NDM-1 , ampC and armA -using oligonucleotide specific primers as given in Table 2 (Pagani et al, 2003;Galimand et al, 2003;Versalovic et al, 1991;Guessennd et al, 2008 presence of integron was screened as described elsewhere (Guerra et al, 2000;Galimand et al, 2003). The amplified products were sequenced using an ABI 3130 genetic analyser (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

Prevalence of IncI1-Iγ and IncFIA-FIB type plasmids in extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains isolated from the NICU of a North Indian hospital

Ali

Khan

2014

Microbiology

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We studied the molecular mechanism of resistance in extended-spectrum b-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from a neonatal intensive care unit (NICU) of one of the hospitals in North India. A total of 3000 clinical samples were collected from a NICU (January 2009 to February 2011), of which 523 strains were K. pneumoniae positive and 262 of them were ESBL-producing K. pneumoniae strains. All of the ESBL-producing clinical isolates were susceptible to carbapenems. However, the majority of the clinical isolates (30-96 %) were resistant to a wide range of antibiotics including antibiotic/inhibitor combinations. The MIC values confirmed that these isolates were highly resistant to cephalosporins and aztreonam. In the 262 ESBL-producing K. pneumoniae isolates, 15 different enterobacterial repetitive intergenic consensus (ERIC)-PCR-typed phylogenetic groups were identified and reconfirmed by PFGE. Characterization of plasmids from each representative member of these phylogenetic groups revealed the presence of three plasmids of different sizes. Conjugation experiments confirmed the presence of different resistance markers only on the 154 kb plasmid. PCR amplification and sequence analysis revealed that bla CTX-M-3 , bla TEM-1 , bla SHV-1 , bla OXA-1 and armA were the predominant resistance markers. Plasmid-replicon typing showed that IncI1-Ic and IncFIA-FIB types are the most prevalent. This study shows the co-existence of multiple ESBL-encoding genes and their polyclonal dissemination among K. pneumoniae clinical isolates in the NICU of a North Indian hospital.

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Section: Methodsmentioning

confidence: 99%

Prevalence of IncI1-Iγ and IncFIA-FIB type plasmids in extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains isolated from the NICU of a North Indian hospital

Ali

Khan

2014

Microbiology

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“…Seven types of 16S rRNA methylases have been identified: ArmA, RmtA, RmtB, RmtC, RmtD, RmtE and NpmA (Wachino et al, 2010). The ArmA methylase was first reported in a strain of Klebsiella pneumoniae in France (Galimand et al, 2003). Subsequently, ArmA methylase-producing A. baumannii were detected in Korea (Lee et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Diverse carbapenem-resistance mechanisms in 16S rRNA methylase-producing Acinetobacter baumannii

Yamada

Suwabe

2013

Journal of Medical Microbiology

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Multidrug-resistant Acinetobacter baumannii has become a global problem. This study characterized amikacin-resistant A. baumannii isolated from eight patients during April 2010-March 2011 in our university hospital and examined the possible mechanisms in three cases in which carbapenem susceptibility changed to carbapenem resistance during treatment of the patients. The armA gene, which is one of the 16S rRNA methylase genes and is associated with high MICs of aminoglycosides, was positive in all isolates. The bla OXA-51 gene and ISAba1 and ISAba1-bla ADC were positive in all isolates, but ISAba1-bla OXA-51 was positive in only three isolates. The CarO outer-membrane protein was lost in one isolate. In the first case, both the susceptible and the resistant isolates were positive for ISAba1-bla OXA-51 , but the expression of the bla ADC gene was increased 3.1-fold in the carbapenem-resistant isolate of the pair. In the second case, the carbapenem-resistant strain became positive for ISAba1-bla OXA-51 , resulting in 21.5-fold increased expression of bla OXA-51 , compared to the carbapenem-susceptible strain of the pair. In the third case, the carbapenemase genes remained negative despite the carbapenem resistance, but the expression of the adeB gene was increased 4.6-fold after acquisition of carbapenem resistance. Multilocus sequence typing analysis of two isolates showing representative pulsed-field gel electrophoresis patterns demonstrated that both isolates were classified to sequence type 2 (ST2). These results showed that the 16S rRNA methylaseproducing A. baumannii, initially susceptible to carbapenem, acquired carbapenem resistance via diverse mechanisms.

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“…Production of 16S rRNA methylase had been reported among aminoglycoside-producing actinomycetes, including Micromonospora spp. and Streptomyces spp., but this novel aminoglycoside resistance mechanism had not been identified in clinical pathogens before, although a similar putative 16S rRNA methylase, ArmA, was found quite recently in Klebsiella pneumoniae in Europe (11). In the present study, we investigated the genetic environments of the rmtA genes harbored by two different P. aeruginosa strains isolated in separate Japanese hospitals.…”

mentioning

confidence: 99%

Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

Yamane

Doi

Yokoyama

et al. 2004

Antimicrob Agents Chemother

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Nine Pseudomonas aeruginosa strains showing very high levels of resistance to various aminoglycosides have been isolated from clinical specimens in seven separate Japanese hospitals in five prefectures since 1997. These strains harbor the newly identified 16S rRNA methylase gene (rmtA). When an rmtA gene probe was hybridized with genomic DNAs of the nine strains digested with EcoRI, two distinct patterns were observed. The 11.1-and 15.8-kb regions containing the rmtA genes of strains AR-2 and AR-11, respectively, were sequenced and compared. In strain AR-2, a transposase gene-like sequence (sequence 1) and a probable tRNA ribosyltransferase gene (orfA) were located upstream of rmtA, and a Na ؉ /H ؉ antiporter gene-like sequence (sequence 2) was identified downstream of rmtA. This 6.2-kbp insert (the rmtA locus) was flanked by 262-bp ␥ elements. Part of the orfQ gene adjacent to an inverted repeat was found outside of the rmtA locus. In strain AR-11, the rmtA gene and sequence 2 were found, but the 5 end of the orfA gene was truncated and replaced with IS6100. An orfQ-orfI region was present on each side of the rmtA gene in strain AR-11. The G؉C content of the rmtA gene was about 55%, and since the newly identified rmtA gene may well be mediated by some mobile genetic elements such as Tn5041, further dissemination of the rmtA gene could become an actual clinical problem in the near future.

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Plasmid-Mediated High-Level Resistance to Aminoglycosides in Enterobacteriaceae Due to 16S rRNA Methylation

Cited by 312 publications

References 47 publications

Prevalence of IncI1-Iγ and IncFIA-FIB type plasmids in extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains isolated from the NICU of a North Indian hospital

Prevalence of IncI1-Iγ and IncFIA-FIB type plasmids in extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains isolated from the NICU of a North Indian hospital

Diverse carbapenem-resistance mechanisms in 16S rRNA methylase-producing Acinetobacter baumannii

Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates

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