Plasmid-mediated doxycycline resistance in a Yersinia pestis strain isolated from a rat

Cabanel, Nicolas; Bouchier, Christiane; Rajerison, Minoarisoa; Carniel, Élisabeth

doi:10.1016/j.ijantimicag.2017.09.015

Cited by 56 publications

(49 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, the rapid induction of protective immunity against bacterial infections could also be used for prophylaxis in combination with second-line antibiotics in cases of antibiotic resistance to the first-choice therapy. 7 , 8 The recent outbreak of pneumonic plague on the island of Madagascar 4 and the concern regarding the appearance of antibiotic-resistant Y. pestis strains further emphasize the benefits of the development of such countermeasures.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Targeting of the Yersinia pestis F1 capsular antigen by innate-like B1b cells mediates a rapid protective response against bubonic plague

et al. 2018

View full text Add to dashboard Cite

The generation of adaptive immunity by vaccination is usually a prolonged process that requires multiple dosing over several months. Hence, vaccines are administered for disease prevention a relatively long time prior to possible infection as opposed to post-exposure prophylaxis, which typically requires rapid intervention such as antibiotic therapy. The emergence of pathogens resistant to common antibiotic treatments has prompted the search for alternative therapeutic strategies. We previously demonstrated that vaccination of mice with the F1 capsular antigen of Yersinia pestis elicits specific and effective yet, unexpectedly, rapid anti-plague immunity. Here, we show by applying genetic and immunological approaches that the F1 antigen is targeted by peritoneal innate-like B1b cells that generate a prompt T-independent (TI) anti-F1 humoral response. The rapid F1-mediated defense response was diminished in Xid (Btkm) mice in which B1 cell numbers and activity are limited. Binding of fluorophore-labeled F1 to peritoneal B1b cells was detected as soon as 6 h post vaccination, emphasizing the high speed of this process. By assessing the ability to achieve rapid immunity with monomerized F1, we show that the natural polymeric structure of F1 is essential for (i) rapid association with peritoneal B1b cells, (ii) early induction of anti-F1 titers and (iii) rapid TI immunity in the mouse model of bubonic plague. These observations shed new light on the potential of novel as well as well-known protective antigens in generating rapid immunity and could be implemented in the rational design of future vaccines.

show abstract

Section: Discussionmentioning

confidence: 99%

“… 6 The recent isolation of virulent Y. pestis strains, which are resistant to several antibiotics including those recommended by the Centers for Disease Control and Prevention (CDC) for therapy, highlighted the need for additional countermeasures. 7 , 8 …”

Section: Introductionmentioning

confidence: 99%

Targeting of the Yersinia pestis F1 capsular antigen by innate-like B1b cells mediates a rapid protective response against bubonic plague

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The possibility of Y. pestis to exchange resistant coding plasmids with other members of the Enterobacteriaceae family, which can easily carry almost any sort of drug resistance, is a serious threat [78]. Y. pestis has been shown to be sensitive to antibiotics in strains isolated from humans; however, 3 unrelated strains have been isolated from hosts and vectors in Madagascar which carried plasmid transmitted resistance to streptomycin and other drugs [79,80]. The extreme virulence of Y. pestis further stresses the need to set up reliable monitoring of susceptibility to treatment.…”

Section: How Can Plague Surveillance and Case Management Be Improved?mentioning

confidence: 99%

Human plague: An old scourge that needs new answers

et al. 2020

Self Cite

View full text Add to dashboard Cite

Yersinia pestis, the bacterial causative agent of plague, remains an important threat to human health. Plague is a rodent-borne disease that has historically shown an outstanding ability to colonize and persist across different species, habitats, and environments while provoking sporadic cases, outbreaks, and deadly global epidemics among humans. Between September and November 2017, an outbreak of urban pneumonic plague was declared in Madagascar, which refocused the attention of the scientific community on this ancient human scourge. Given recent trends and plague's resilience to control in the wild, its high fatality rate in humans without early treatment, and its capacity to disrupt social and PLOS NEGLECTED TROPICAL DISEASES

show abstract

“…Most B. anthracis strains are susceptible to antibiotics, but surveys of clinical and environmental isolates indicate penicillin resistance occurs in 2 to 16% of strains 7 Attenutated B. anthracis strains with AMR were isolated following laboratory in vitro passaging (selective pressure) and/or targeted genetic manipulation 8, 9 Laboratory-selected virulent and avirulent Y. pestis strains with quinolone-resistance were previously reported 10, 11 and engineered multi-drug resistant (MDR) strains are a public health concern 12, 13 . Y. pestis is intrinsically susceptible to every antibiotic recommended for human plague therapy, but a few Y. pestis isolates with transferable plasmid-mediated resistance were reported from Madagascar 13, 14, 15, 16 .…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Genetic Engineering, Structural Variation, and Antimicrobial Resistance Markers in Bacterial Biothreat Pathogens by Nanopore Sequencing

Gargis

Cherney

Conley

et al. 2019

Preprint

View full text Add to dashboard Cite

Widespread release of Bacillus anthracis (anthrax) or Yersinia pestis (plague) would prompt a 24 public health emergency. During an exposure event, high-quality whole genome sequencing (WGS) can 25 identify genetic engineering, including the introduction of antimicrobial resistance (AMR) genes. Here, 26 we developed rapid WGS laboratory and bioinformatics workflows using a long-read nanopore 27 sequencer (MinION) for Y. pestis (6.5h) and B. anthracis (8.5h) and sequenced strains with different 28 AMR profiles. Both salt-precipitation and silica-membrane extracted DNA were suitable for MinION 29 WGS using both rapid and field library preparation methods. In replicate experiments, nanopore quality 30 metrics were defined for genome assembly and mutation analysis. AMR markers were correctly 31 detected and >99% coverage of chromosomes and plasmids was achieved using 100,000 raw sequencing 32 reads. While chromosomes and large and small plasmids were accurately assembled, including novel 33 multimeric forms of the Y. pestis virulence plasmid, pPCP1, MinION reads were error-prone, 34 particularly in homopolymer regions. MinION sequencing holds promise as a practical, front-line 35 strategy for on-site pathogen characterization to speed the public health response during a biothreat 36 emergency. 37 38 40respectively. Deliberate misuse of these pathogens as bioterrorism agents poses a serious public health 41 and safety threat, due to low infectious doses, high lethality, and the ease of production, dissemination 42 and communicability 1 . Strains of both species were weaponized previously, and the 2001 Amerithrax 43 incident underscores the importance of biological threat preparedness and rapid laboratory response 2, 3 . 44Natural anthrax and plague outbreaks are reported annually in animals, and human cases are often 45 zoonotic. While these diseases continue to cycle in isolated, enzootic foci, re-emergence was reported 46 3 during 2016 anthrax outbreaks in Russia and Sweden 4 , and the 2017 pneumonic plague outbreak in 47 Madagascar 5 . Both naturally-occurring and engineered antimicrobial resistance (AMR) have been 48 reported in Y. pestis and B. anthracis 6 . Most B. anthracis strains are susceptible to antibiotics, but 49 surveys of clinical and environmental isolates indicate penicillin resistance occurs in 2 to 16% of strains 50 7 . Attenutated B. anthracis strains with AMR were isolated following laboratory in vitro passaging 51 (selective pressure) and/or targeted genetic manipulation 8, 9 . Laboratory-selected virulent and avirulent 52 Y. pestis strains with quinolone-resistance were previously reported 10, 11 and engineered multi-drug 53 resistant (MDR) strains are a public health concern 12, 13 . Y. pestis is intrinsically susceptible to every 54 antibiotic recommended for human plague therapy, but a few Y. pestis isolates with transferable 55 plasmid-mediated resistance were reported from Madagascar 13, 14, 15, 16 . 56 A public health response to incidents involving human plague or anthrax requires ...

show abstract

Plasmid-mediated doxycycline resistance in a Yersinia pestis strain isolated from a rat

Cited by 56 publications

References 17 publications

Targeting of the Yersinia pestis F1 capsular antigen by innate-like B1b cells mediates a rapid protective response against bubonic plague

Targeting of the Yersinia pestis F1 capsular antigen by innate-like B1b cells mediates a rapid protective response against bubonic plague

Human plague: An old scourge that needs new answers

Rapid Detection of Genetic Engineering, Structural Variation, and Antimicrobial Resistance Markers in Bacterial Biothreat Pathogens by Nanopore Sequencing

Contact Info

Product

Resources

About