Plasmid-mediated colistin resistance in Latin America and Caribbean: A systematic review

Oliveira, Victor Rocha Mendes; Paiva, Magna Cristina de; Lima, William Gustavo

doi:10.1016/j.tmaid.2019.07.015

Cited by 35 publications

(22 citation statements)

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“…Plasmid mediated colistin resistance has been described worldwide in a variety of Enterobacterales of different origins, environment, food producing animals, wildlife and humans (Zurfluh et al, 2016;Guenther et al, 2017;Ovejero et al, 2017;Barlaam et al, 2019;Elbediwi et al, 2019;Lalaoui et al, 2019;Mendes Oliveira et al, 2019;Nang et al, 2019;Zając et al, 2019). In particular, in Spain, mcr-1 has been described circulating in pig farms for many years (Quesada et al, 2016;Garcia-Menino et al, 2018), and our results demonstrate its presence in pig farms in Spain as early as 2005.…”

Section: Discussionsupporting

confidence: 54%

mcr-Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain

et al. 2020

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Colistin has become the last-line antimicrobial for the treatment of multidrug resistant (MDR) Enterobacterales in human medicine. To date, several colistin resistance genes have been described. Of them mcr-1 is disseminated worldwide in Escherichia coli of human and animal origin. The aim of this study was to characterize mcr-mediated resistance plasmids from E. coli of animal origin in Spain. From our strain collection, 70 E. coli of pig origin collected between 2005 and 2014 (10 per year, except for years 2009-2010-2013) were randomly selected and screened for the presence of mcr-genes. Additionally, 20 E. coli isolated in 2011 from white storks (Ciconia ciconia) from the same urban household waste landfill associated colony were also included. Whole genome sequencing of mcr-positive isolates was carried out on a MiSeq (Illumina). Hybrid whole genome sequencing strategy combining nanopore and Illumina technologies were performed in a selection of isolates to close the genomes and plasmids and identify the presence of antimicrobial resistance genes. Minimum inhibitory concentration (MIC) was used to assess the susceptibility to colistin. Mating experiments were carried out to evaluate transferability of the mcr-genes. A total of 19 mcr-1 and one mcr-4 positive isolates were detected, 15 from pigs distributed during the study period, and five from storks collected in 2011. No other mcr-variants were found. The MICs for colistin ranged between 4 and >4 mg/L. High diversity of STs were detected among the mcr-1 positive E. coli isolates, with only ST-10 shared between pigs and white storks. Except for one isolate, all were genotypic and phenotypically MDR, and five of them also harbored cephalosporin resistance genes (bla CTX−M−14 , bla SHV−12 , and three bla CMY−2). mcr-1 genes were mobilizable by conjugation, associated with IncX4, IncHI2, and IncI2 plasmids. In our study, mcr-1 genes have been circulating in pig farms since 2005 harbored by a variety of E. coli clones. Its persistence may be driven by co-selection since plasmids containing mcr-1 also exhibit resistance to

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Section: Discussionsupporting

confidence: 54%

mcr-Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain

et al. 2020

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“…This gene encodes a plasmid-borne phosphoethanolamine transferase that has been reported in Escherichia coli isolates from animal, food, environment, and human samples worldwide ( 3 , 4 ). At this time, nine mcr genes have been described; but, mcr-1 is, by far, the most prevalent ( 3 - 5 ). Mcr-1 has been described in almost all countries in the Region of the Americas, while mcr-3 and mcr-5 genes have been sporadically described in only Brazil and Colombia, respectively ( 3 , 5 – 7 ).…”

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confidence: 99%

“…At this time, nine mcr genes have been described; but, mcr-1 is, by far, the most prevalent ( 3 - 5 ). Mcr-1 has been described in almost all countries in the Region of the Americas, while mcr-3 and mcr-5 genes have been sporadically described in only Brazil and Colombia, respectively ( 3 , 5 – 7 ). Although detected in other Enterobacterales , the mcr-1 gene has been associated mainly with E. coli isolates, including Klebsiella pneumoniae and Salmonella spp.…”

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confidence: 99%

“…Although detected in other Enterobacterales , the mcr-1 gene has been associated mainly with E. coli isolates, including Klebsiella pneumoniae and Salmonella spp. ( 5 ). Regardless of species, mcr-1 has been associated with a ~2609 bp DNA fragment containing the mcr-1 and pap2 genes, and has mobilized into an ISApI1 -based composite transposon and different plasmid replicons, of which IncI2, IncX4, and IncHI2 are the most common incompatibility groups described so far ( 8 - 10 ).…”

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confidence: 99%

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Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018

Faccone¹,

Rapoport²,

Albornoz³

et al. 2020

Revista Panamericana De Salud Pública

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Objective. To describe the resistance profile and the genetic characteristics of Escherichia coli isolates that harbor the mobilizable colistin resistance gene mcr-1 in Argentina. Methods. This was a retrospective study of 192 E. coli isolates positive for mcr-1 obtained from 69 hospitals of Buenos Aires City and 14 Argentinean provinces in 2012 – 2018. The antimicrobial susceptibility was performed by agar diffusion, broth macrodilution, and/or agar dilution. Standard polymerase chain reaction (PCR) was performed to detect resistance genes and incompatibility groups; specific PCR was applied to discriminate between blaCTX-M allelic groups and mcr-1.5 variant. The genetic relatedness among isolates was evaluated by XbaI-pulsed field gel electrophoresis and multilocus sequence typing in a subset of isolates. Results. All E. coli isolates showed minimal inhibitory concentrations to colistin = 4µg/mL; nearly 50% were resistant to third-generation cephalosporins, with CTX-M-2 being the main extended-spectrum β-lactamase detected. Five E. coli were carbapenemase-producers (3 NDM, 2 KPC). The mcr-1.5 variant was detected in 13.5% of the isolates. No genetic relationship was observed among the mcr-1-positive E. coli clinical isolates, but a high proportion (164/192; 85.4%) of IncI2 plasmids was detected. Conclusions. The presence of IncI2 plasmids among highly diverse E. coli clones suggests that the mcr-1 gene’s wide distribution in Argentina may be driven by the horizontal transmission of IncI2 plasmids.

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“…coli mcr-1+ em diversas fontes e em vários países (KATSUNUMA et al, 2007;GALES;JONES;SADER, 2011;KEMPF et al, 2013;LIM et al, 2016), incluindo o Brasil (CORRÊA, 2013;CUNHA, 2018;MENDES OLIVEIRA;PAIVA;LIMA, 2019).…”

Section: Dentre Os Antimicrobianos Os Antibióticos Classificados Pelunclassified

>b<Marcadores epidemiológicos de resistência à colistina em >i<Escherichia coli>/i< de origem aviária:>/b< análise crítica sobre biosseguridade e uso de antimicrobianos em avicultura no Brasil

Reple¹

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Ao universo, refletido para mim em Deus e em todas as entidades que acredito, que sempre me apresenta a pessoas incríveis ao longo do caminho e me presenteia com saúde, coragem e resiliência diariamente. À minha família, por toda compreensão e apoio durante esta jornada. Em especial à minha mãe por ser exemplo de dedicação e por seu amor incondicional; à minha irmã Marina, companheira dessa e de outras vidas; à minha irmã de coração, Bruna e aos meus avós, Elza e Orlando, que são pessoas íntegras e do bem, fontes de experiência e sabedoria. A todos meus amigos; dentre eles Joca, Kiz, Nou, Pin e Kika, que estão sempre ao meu lado, me escutando, dando forças e deixando a vida mais leve. À minha orientadora Profa. Dra. Terezinha Knöbl, a quem tenho como inspiração profissional e pessoal, por toda sua sábia orientação e conselhos, pela paciência e confiança em mim. À toda a equipe do Laboratório de Sanidade Suína e do Laboratório de Medicina Aviária da FMVZ-USP, em especial à Fernanda, Leticia, Vitória, Yamê e Gabi que me auxiliaram com os experimentos e, sem dúvidas, ao Marcos que, além de ter me orientado de forma precisa e compreensiva, tive a feliz oportunidade de ter aprendido muito com ele. À Vanessa, por ter me auxiliado de forma inestimável e com muito profissionalismo durante a etapa final das análises. Ao Programa de Pós-Graduação em Patologia Experimental e Comparada e à Elanco, por terem possibilitado aprimorar meus conhecimentos técnicos. A todos os veterinários, técnicos de campo e granjeiros das integrações, que me receberam de portas abertas e com disposição para ajudar, possibilitando a realização do presente trabalho. "Se vi mais longe, foi por estar de pé sobre ombros de gigantes." (Isaac Newton) RESUMO REPLE, J.N. Marcadores epidemiológicos de resistência à colistina em Escherichia coli de origem aviária: análise crítica sobre biosseguridade e uso de antimicrobianos em avicultura no Brasil. 2020. 99 f. Dissertação (Mestrado em

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Plasmid-mediated colistin resistance in Latin America and Caribbean: A systematic review

Cited by 35 publications

References 67 publications

mcr-Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain

mcr-Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain

Plasmidic resistance to colistin mediated by mcr-1 gene in Escherichia coli clinical isolates in Argentina: A retrospective study, 2012–2018

>b<Marcadores epidemiológicos de resistência à colistina em >i<Escherichia coli>/i< de origem aviária:>/b< análise crítica sobre biosseguridade e uso de antimicrobianos em avicultura no Brasil

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