Plasmid-encoded amikacin resistance in multiresistant strains of Klebsiella pneumoniae isolated from neonates with meningitis

Woloj, Mabel; Tolmasky, Marcelo E.; Roberts, Marilyn C.; Crosa, Jorge H.

doi:10.1128/aac.29.2.315

Cited by 59 publications

(79 citation statements)

References 33 publications

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“…Plasmid pJHC-MW1 confers resistance to amikacin on Klebsiella pneumoniae (22). We have recently cloned the genetic determinants for this resistance, which are included within a 1.5-kilobase'(kb) BamHI fragment of pJHC-MW1 (20).…”

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confidence: 99%

Sequencing and expression of the 6'-N-acetyltransferase gene of transposon Tn1331 from Klebsiella pneumoniae

et al. 1988

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Plasmid-mediated amikacin resistance in Klebsiella pneumoniae resides on a 1.5-kilobase BamHI fragment which is part of the Tn3-related multiresistance transposon Tn1331. In this work, we present the complete nucleotide sequence of the amikacin resistance gene and the neighboring sequences. Maxicell experiments detected only one polypeptide of 23 kilodaltons, the product of one of the open reading frames identified as ORF I. Comparison of the complete sequence with that of Tn3 indicated that 396 base pairs located just upstream from ORF I are identical to a region between the end of the tnpR gene and the first six amino acids of the beta-lactamase transcript. Sequences which may act as hot spots for recombination were identified. One was located just after amino acid 6 of beta-lactamase, and the other was located at the end of the amikacin resistance gene.

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Sequencing and expression of the 6'-N-acetyltransferase gene of transposon Tn1331 from Klebsiella pneumoniae

et al. 1988

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“…The multiresistance plasmid pJHCMW1, originally isolated from a clinical K. pneumoniae strain, includes the Xer recombination site mwr (37,49,54). Our previous work showed an inverse relationship between osmolarity of the growth medium and efficiency of resolution by Xer recombination at mwr in E. coli strains (34).…”

Section: Discussionmentioning

confidence: 99%

“…This plasmid also harbors the transposon Tn1331, which specifies resistance to several aminoglycosides and ␤-lactams (37,48,50,51,54). Resolution of dimers harboring mwr is inefficient when the Escherichia coli host cells are cultured in L broth (34).…”

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Differences in Resolution of mwr -Containing Plasmid Dimers Mediated by the Klebsiella pneumoniae and Escherichia coli XerC Recombinases: Potential Implications in Dissemination of Antibiotic Resistance Genes

et al. 2006

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Xer-mediated dimer resolution at the mwr site of the multiresistance plasmid pJHCMW1 is osmoregulated in Escherichia coli containing either the Escherichia coli Xer recombination machinery or Xer recombination elements from K. pneumoniae. In the presence of K. pneumoniae XerC (XerC Kp ), the efficiency of recombination is lower than that in the presence of the E. coli XerC (XerC Ec ) and the level of dimer resolution is insufficient to stabilize the plasmid, even at low osmolarity. This lower efficiency of recombination at mwr is observed in the presence of E. coli or K. pneumoniae XerD proteins. Mutagenesis experiments identified a region near the N terminus of XerC Kp responsible for the lower level of recombination catalyzed by XerC Kp at mwr. This region encompasses the second half of the predicted ␣-helix B and the beginning of the predicted ␣-helix C. The efficiencies of recombination at other sites such as dif or cer in the presence of XerC Kp or XerC Ec are comparable. Therefore, XerC Kp is an active recombinase whose action is impaired on the mwr recombination site. This characteristic may result in restriction of the host range of plasmids carrying this site, a phenomenon that may have important implications in the dissemination of antibiotic resistance genes.

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“…The 1.4-kb HindIII fragment in pTK1597 as well as the 2.0-kb BamHIHindIII fragment in pTK1598 will provide tools for this exploration. Meanwhile, the AMK-resistance region of pJHCMW1, a plasmid from Klebsiella pneumoniae strain JHCK1 (30), contains a single site for Pvul, Kpnl, and SstI as well as two sites for BstEII (27). The region of Tn2401, which is a transposon found in a strain of Pseudomonas aeruginosa and specifies the AAC(6') gene as well as the gene for aminoglycoside 3'-phosphotransferase I, contains a single site for Bgll, Bg111, HindIII, and Sall (25), and that of Tn2424 encoding AAC(6') has one site for BgIII and Sall (19).…”

Section: Discussionmentioning

confidence: 99%

Physical and Functional Map of an Amikacin‐Resistance Plasmid Isolated from a Multiresistant Strain of Serratia marcescens

Kanno¹

1988

Microbiology and Immunology

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pTK159, a multiresistance 40-kilobase (kb) plasmid, was isolated from a clinical strain of Serratia marcescens. pTK159 was nonconjugative and carried determinants for resistance to amikacin, streptomycin/spectinomycin, sulfamethoxazole and ampicillin. A physical and functional map of pTK159 was constructed. By cloning various fragments of pTK 159 in pACYC 184 or pBR322, genes for resistance to amikacin, streptomycin/spectinomycin, and sulfamethoxazole were found to be located on a 2.0-kb BamHI-HindIII fragment, a 1.4-kb HindIII fragment and a 2.1-kb HindIII fragment, respectively. The map of pTK159 was compared with published maps of amikacin-resistance determinants and transposons.

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Plasmid-encoded amikacin resistance in multiresistant strains of Klebsiella pneumoniae isolated from neonates with meningitis

Cited by 59 publications

References 33 publications

Sequencing and expression of the 6'-N-acetyltransferase gene of transposon Tn1331 from Klebsiella pneumoniae

Sequencing and expression of the 6'-N-acetyltransferase gene of transposon Tn1331 from Klebsiella pneumoniae

Differences in Resolution of mwr -Containing Plasmid Dimers Mediated by the Klebsiella pneumoniae and Escherichia coli XerC Recombinases: Potential Implications in Dissemination of Antibiotic Resistance Genes

Physical and Functional Map of an Amikacin‐Resistance Plasmid Isolated from a Multiresistant Strain of Serratia marcescens

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