Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.02.028 View full text
Hans-Olof Johansson, Tiago Matos, Juliana S. Luz, Eloi Feitosa, Carla C. Oliveira, Adalberto Pessoa, Leif Bülow, Folke Tjerneld

Abstract: Phase diagrams of poly(ethylene glycol)/polyacrylate/Na(2)SO(4) systems have been investigated with respect to polymer size and pH. Plasmid DNA from Escherichia coli can depending on pH and polymer molecular weight be directed to a poly(ethylene glycol) or to a polyacrylate-rich phase in an aqueous two-phase system formed by these polymers. Bovine serum albumin (BSA) and E. coli homogenate proteins can be directed opposite to the plasmid partitioning in these systems. Two bioseparation processes have been deve…

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