Plasmid DNA partitioning and separation using poly(ethylene glycol)/poly(acrylate)/salt aqueous two-phase systems

Johansson, Helena; Matos, Tiago; Luz, Juliana Silva da; Feitosa, Eloi; Oliveira, Carla C.; Pessoa, Adalberto; Bülow, Leif; Tjerneld, Folke

doi:10.1016/j.chroma.2012.02.028

Cited by 26 publications

(16 citation statements)

References 29 publications

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“…The APTS have been widely used in extracting and separating bioactive substance [10][11][12], organic small molecule [13][14][15] and metal cations [16][17][18][19]. However, only a few outcomes have been reported in the extraction and separation of metal anion by ATPS.…”

Section: Introductionmentioning

confidence: 99%

A green method for extracting molybdenum (VI) from aqueous solution with aqueous two-phase system without any extractant

Zhang

Sun

Hou

et al. 2016

Separation and Purification Technology

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Section: Introductionmentioning

confidence: 99%

A green method for extracting molybdenum (VI) from aqueous solution with aqueous two-phase system without any extractant

Zhang

Sun

Hou

et al. 2016

Separation and Purification Technology

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“…In a previous study, when pDNA was dissolved in a mixture of hydrophilic PEG 8000 and hydrophobic polyacrylate, the pDNA was partitioned in the polyacrylate at a low pH <5.8. 17) This indicates that the hydrophobicity of pDNA is elevated at a low pH. Therefore, it would not be possible to encapsulate the pDNA in the YSK-MEND at a pH<4.0.…”

Section: Discussionmentioning

confidence: 99%

Efficient Packaging of Plasmid DNA Using a pH Sensitive Cationic Lipid for Delivery to Hepatocytes

Sakurai

Matsuda

Hada

et al. 2015

Biological & Pharmaceutical Bulletin

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Plasmid DNA (pDNA) is expected to be a new class of medicine for treating currently incurable diseases. To deliver these nucleic acids, we developed a liposomal delivery system we have called a multifunctional envelope-type nano device (MEND). In this report, we demonstrate that a MEND containing a pH-sensitive cationic lipid, YSK05 (YSK-MEND), efficiently delivered pDNA via systemic injection, and that its expression was highly dependent on the encapsulation state of the pDNA. In the preparation, the pH, ionic strength, and sodium chloride (NaCl) concentration of the lipid/pDNA mixture strongly affected the encapsulation efficiency of pDNA. Additionally, the transgene expression of luciferase in the liver by the injected YSK-MEND was dependent on the encapsulation state of pDNA rather than the nature of the YSK-MEND. Confocal laser scanning microscopy findings revealed that injection of the YSK-MEND led to homogenous gene expression in the liver compared to injection via the hydrodynamic tail vein (HTV). Concerning the safety of the YSK-MEND, a transient increase in the activity of liver enzymes was observed. However, no significant adverse events were observed. Taken together, the YSK-MEND represents a potentially attractive therapy for the treatment of various hepatic diseases.

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“…The molecular recognition between nucleic acids and surrounding molecules therefore involves several different modalities, which need to be taken into consideration when designing a purification process. Several alternative strategies are available, and purification of DNA can be based on a range of different technologies including various forms of chromatography, but other methods such as precipitation (Freitas et al ., ), filtration (Guerrero‐German et al ., ), and aqueous two‐phase systems (Barbosa et al ., ; Johansson et al ., ; Wiendahl et al ., ) have all been explored. Particularly, liquid chromatography has proved effective and has become an essential operational process for the large‐scale purification of pDNA products, both as a preparative step and/or as an analytical tool in the process (Sousa et al ., ; Guerrero‐German et al ., ; Sousa et al ., ; Mahut et al ., ; Mota et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Plasmid DNA purification using a multimodal chromatography resin

Matos

Queiroz

Bülow

2014

J of Molecular Recognition

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Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. Capto Adhere proved to offer a very strong binding of nucleic acids. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin.

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Plasmid DNA partitioning and separation using poly(ethylene glycol)/poly(acrylate)/salt aqueous two-phase systems

Cited by 26 publications

References 29 publications

A green method for extracting molybdenum (VI) from aqueous solution with aqueous two-phase system without any extractant

A green method for extracting molybdenum (VI) from aqueous solution with aqueous two-phase system without any extractant

Efficient Packaging of Plasmid DNA Using a pH Sensitive Cationic Lipid for Delivery to Hepatocytes

Plasmid DNA purification using a multimodal chromatography resin

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