Plasmid-DNA loaded chitosan microspheres for in vitro IL-2 expression

Akbuğa, Jülide; Özbaş-Turan, Suna; Erdoğan, Neslihan

doi:10.1016/j.ejpb.2004.04.015

Cited by 39 publications

(14 citation statements)

References 20 publications

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“…84 Delivery of growth factors, plasmids, and adenoviral vectors has also been reported. 84,[108][109][110][111][112][113][114][115][116][117][118][119][120][121][122][123][124][125] In contrast, hyaluronan HGs made by physical preparation only have been less extensively studied due to suboptimal mechanical properties, so that a mild chemical crosslinking step was added at the end of the preparation procedure. 84 However, physical gelation with/in presence of collagen, alginate, polylactide-based microspheres, copper ions, and applied for the restoration of vocal fold lamina propria, cartilage TE, and induction of neovascolarization have been reported.…”

Section: Ionic Interactionsmentioning

confidence: 99%

Hydrogels for Tissue Engineering and Delivery of Tissue-Inducing Substances

Baroli

2007

Journal of Pharmaceutical Sciences

151

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Section: Ionic Interactionsmentioning

confidence: 99%

Hydrogels for Tissue Engineering and Delivery of Tissue-Inducing Substances

Baroli

2007

Journal of Pharmaceutical Sciences

151

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“…The particle size is recognized as a key parameter since it may affect the blood circulation time and the cellular uptake Wolfert and Seymor, 1996]. However the experimental results available are contradictory and reasonable transfection efficiencies were reported for particles having sizes varying from 100 nm [Erbacher et al, 1998] to 2.0 micrometers [Akbuga andOzbas Turan, 2004, [Liu et al, 2003]]. …”

Section: Chitosanmentioning

confidence: 99%

Synthetic and Natural Polycations for Gene Therapy: State of the Art and New Perspectives

Tiera¹,

Winnik²,

Fernandes³

2006

CGT

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Currently, the major drawback of gene therapy is the gene transfection rate. The two main types of vectors that are used in gene therapy are based on viral or non-viral gene delivery systems. There are several non-viral systems that can be used to transfer foreign genetic material into the human body. In order to do so, the DNA to be transferred must escape the processes that affect the disposition of macromolecules. These processes include the interaction with blood components, vascular endothelial cells and uptake by the reticuloendothelial system. Furthermore, the degradation of therapeutic DNA by serum nucleases is also a potential obstacle for functional delivery to the target cell. Cationic polymers have a great potential for DNA complexation and may be useful as non-viral vectors for gene therapy applications. The objective of this review was to address the state of the art in gene therapy using synthetic and natural polycations and the latest strategies to improve the efficiency of gene transfer into the cell.

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“…The particle size is recognized as a key parameter since it may affect the blood circulation time and the cellular uptake [15][16][17][18]. Several strategies have been employed aiming to enhance the efficiency of chitosan-DNA nanoparticles as vectors in gene therapy by decreasing their interaction with blood components and their uptake by the reticuloendothelial system.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characterization of nanoparticles formed between DNA and phosphorylcholine substituted chitosans

Casé

Picola

Zaniquelli

et al. 2009

Journal of Colloid and Interface Science

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The interactions between phosphorylcholine-substituted chitosans (PC-CH) and calf-thymus DNA (ct-DNA) were investigated focusing on the effects of the charge ratio, the pH, and phosphorylcholine content on the size and stability of the complexes using the ethidium bromide fluorescence assay, gel electrophoresis, dynamic light scattering, and fluorescence microscopy. The size and colloidal stability of deacetylated chitosan (CH/DNA) and PC-CH/DNA complexes were strongly dependent on phosphorylcholine content, charge ratios, and pH. The interaction strengths were evaluated from ethidium bromide fluorescence, and, at N/P ratios higher than 5.0, no DNA release was observed in any synthesized PC-CH/DNA polyplexes by gel electrophoresis. The PC-CH/DNA polyplexes exhibited a higher resistance to aggregation compared to deacetylated chitosan (CH) at neutral pH. At low pH values highly charged chitosan and its phosphorylcholine derivatives had strong binding affinity with DNA, whereas at higher pH values CH formed large aggregates and only PC-CH derivatives were able to form small nanoparticles with hydrodynamic radii varying from 100 to 150 nm. Nanoparticles synthesized at low ionic strength with PC-CH derivatives containing moderate degrees of substitution (DS=20% and 40%) remained stable for weeks. Photomicroscopies also confirmed that rhodamine-labeled PC(40)CH derivative nanoparticles presented higher colloidal stability than those synthesized using deacetylated chitosan. Accordingly, due to their improved physicochemical properties these phosphorylcholine-modified chitosans provide new perspectives for controlling the properties of polyplexes.

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Plasmid-DNA loaded chitosan microspheres for in vitro IL-2 expression

Cited by 39 publications

References 20 publications

Hydrogels for Tissue Engineering and Delivery of Tissue-Inducing Substances

Hydrogels for Tissue Engineering and Delivery of Tissue-Inducing Substances

Synthetic and Natural Polycations for Gene Therapy: State of the Art and New Perspectives

Physicochemical characterization of nanoparticles formed between DNA and phosphorylcholine substituted chitosans

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