Plasmid-Determined Resistance to Erythromycin and Lincomycin in Streptococcus Pyogenes

Malke, Horst; Тотолян, А.; Kolesnichenko, T. G.; Golubkov, V. I.; Starke, Renate

doi:10.1007/978-3-642-81064-0_14

Plasmids 1977

DOI: 10.1007/978-3-642-81064-0_14

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Plasmid-Determined Resistance to Erythromycin and Lincomycin in Streptococcus Pyogenes

Horst Malke¹,

А. Тотолян²,

T. G. Kolesnichenko³

et al.

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1981

1982

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Cited by 2 publications

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“…In contrast to lincomycin, erythromycin can act as an inducer of resistance only at low, subinhibitory concentrations, whereas it inhibits protein synthesis at higher concentrations and is therefore unable to induce cells in situ. The (23). Unfortunately, corresponding data are not available for zonal resistance strains, so that at the present state of knowledge, it seems premature to propose a model concerning the mechanism of regulation of resistance.…”

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confidence: 99%

Genetic study of plasmid-associated zonal resistance to lincomycin in Streptococcus pyogenes

Malke¹,

Reichardt²,

Hartmann³

et al. 1981

Antimicrob Agents Chemother

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The phenomenon of zonal resistance to lincomycin, which is characteristic of most clinical isolates with lincomycin resistance in Streptococcus pyogenes, has been studied. These strains grow within a defined concentration range of lincomycin (approximately 60 to 200 microgram/ml), or at lincomycin concentrations below the minimal inhibitory concentration for susceptible strains. It is shown that the zonal growth phenomenon is a stable phenotype and results from induction of resistance only within the zonal concentration range of lincomycin. These strains also possess inducible resistance to erythromycin which is nonzonal in character. One-step mutations to constitutive resistance have been isolated which are of two types: constitutive for lincomycin or for erythromycin, but not for both. Those strains with constitutive erythromycin resistance retain their zonal resistance for lincomycin. Mutants doubly constitutive for both lincomycin and erythromycin can be obtained by a second mutational step from either of the singly constitutive mutants. Satellite deoxyribonucleic acid has been shown to be present in the zonal resistant strains. A plasmid, pSM10419, of 14.9 megadaltons, has been isolated from one of the doubly constitutive mutants and used to jointly transform Streptococcus sanguis strain Challis to constitutive resistance to both lincomycin and erythromycin. From this, a multicopy plasmid of reduced size, pSM10 (5.4 megadaltons), which retains its resistance phenotype, has been isolated and mapped with restriction endonucleases HindIII (three sites), EcoRI (one site), KpnI (one site), and HpaI (one site). The staphylococcal plasmid pC221 (2.9 megadaltons; chloramphenicol resistant) has been fused to pSM10 at the EcoRI site resulting in a chimeric plasmid, pSM10221 (8.3 megadaltons), which retains resistance to chloramphenicol, erythromycin, and lincomycin. pSM10 is therefore suggestive as an effective cloning vehicle for the genus Streptococcus.

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mentioning

confidence: 99%

Genetic study of plasmid-associated zonal resistance to lincomycin in Streptococcus pyogenes

Malke¹,

Reichardt²,

Hartmann³

et al. 1981

Antimicrob Agents Chemother

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Sequence relationships between plasmids associated with conventional MLS resistance and zonal lincomycin resistance in Streptococcus pyogenes

Golubkov

Reichardt²,

Boitsov

et al. 1982

Molec Gen Genet

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By using electron microscopy of self-annealed DNA and restriction enzyme analysis, we have compared the physical maps of two group A streptococcal plasmids associated with conventional MLS resistance (pEL1; 20 Md) and zonal lincomycin resistance (pSM10419; 15 Md). Of their monomeric molecules, about 40% and 60%, respectively, are occupied by identical non-tandem inverted repeats containing sequences specifying putative replication functions. Sequence homology also exists between their resistance determinants which are located in unique DNA. Moreover, homology between additional regions of unknown function is so extensive and restriction fragment arrangement so similar that, formally, pSM10419 can be considered a deletion variant of pEL1. The results suggest that MLS and zonal lincomycin resistance have the same biochemical basis (i.e. methylation of 23S ribosomal RNA) and differ only quantitatively in the inducible control systems.

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Plasmid-Determined Resistance to Erythromycin and Lincomycin in Streptococcus Pyogenes

Cited by 2 publications

References 10 publications

Genetic study of plasmid-associated zonal resistance to lincomycin in Streptococcus pyogenes

Genetic study of plasmid-associated zonal resistance to lincomycin in Streptococcus pyogenes

Sequence relationships between plasmids associated with conventional MLS resistance and zonal lincomycin resistance in Streptococcus pyogenes

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