Plasmid-determined resistance to arsenic and antimony inPseudomonas aeruginosa

Cervantes, Carlos; Chávez, Jaime

doi:10.1007/bf00713941

Cited by 14 publications

(8 citation statements)

References 21 publications

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“…No significant difference in number of heterotrophs and As(V)‐tolerant bacteria was observed in the two soils at the As(V) level examined (0·77 mmol l −1 ) probably for the low toxic effect of the As(V) amount added to TSA/10 medium and the low ratio of arsenic to phosphate, i.e. 0·77–1·6 mmol l −1 (Cervantes and Chavez 1992). Both soils contained high percentage of As(III)‐tolerant bacteria and their percentage was higher in Rice soil (65%) than in Dhal soil (29%).…”

Section: Resultsmentioning

confidence: 99%

Arsenic-resistant bacteria isolated from agricultural soils of Bangladesh and characterization of arsenate-reducing strains

Bachate¹,

Cavalca²,

Andreoni

2009

Journal of Applied Microbiology

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Aims: To analyse the arsenic‐resistant bacterial communities of two agricultural soils of Bangladesh, to isolate arsenic‐resistant bacteria, to study their potential role in arsenic transformation and to investigate the genetic determinants for arsenic resistance among the isolates. Methods and Results: Enrichment cultures were performed in a minimal medium in the presence of As(III) and As(V) to isolate resistant bacteria. Twenty‐one arsenic‐resistant bacteria belonging to different genera of Gram‐positive and Gram‐negative bacteria were isolated. The isolates, with the exception of Oceanimonas doudoroffii Dhal Rw, reduced 2 mmol l−1 As(V) completely to As(III) in aerobic conditions. Putative gene fragments for arsenite efflux pumps were amplified in isolates from Dhal soil and a putative arsenate reductase gene fragment was amplified from a Bacillus sp. from Rice soil. Conclusions: Phylogenetically diverse arsenic‐resistant bacteria present in agricultural soils of Bangladesh are capable of reducing arsenate to arsenite under aerobic conditions apparently for detoxification purpose. Significance and Impact of the Study: This study provides results on identification, levels of arsenic resistance and reduction of arsenate by the bacterial isolates which could play an important role in arsenic cycling in the two arsenic‐contaminated soils in Bangladesh.

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Section: Resultsmentioning

confidence: 99%

Arsenic-resistant bacteria isolated from agricultural soils of Bangladesh and characterization of arsenate-reducing strains

Bachate¹,

Cavalca²,

Andreoni

2009

Journal of Applied Microbiology

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“…Oxidation of As(lit) represents a potential detoxification process that allows microorganisms to tolerate higher levels of arsenite. Several examples of bacterial oxidation of arsenite to arsenate were being reported as early as 1918 (reviewed in [45,1 (13] from an Alcaligenes faecalis strain (Fig. 2).…”

Section: Chromosomal-deterrnined Resistancementioning

confidence: 99%

“…Bacterial resistance to arsenic ions governed by plasmids was first discovered by Novick and Roth [71] in a group of Staphylococcus aureus /3-1actamase plasmids which also determine resistance to heavy metals. Arsenic resistance plasmids confer tolerance to both arsenate and arsenite as well as to antimony(IID [13,71,95]. Resistance to all three ions is inducible and cross-induction among them occurs [71,95].…”

Section: Plasmid-mediated Resistancementioning

confidence: 99%

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Resistance to arsenic compounds in microorganisms

Cervantes

Ramı́rez

et al. 1994

FEMS Microbiology Reviews

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287

143

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Arsenic ions, frequently present as environmental pollutants, are very toxic for most microorganisms. Some microbial strains possess genetic determinants that confer resistance. In bacteria, these determinants are often found on plasmids, which has facilitated their study at the molecular level. Bacterial plasmids conferring arsenic resistance encode specific efflux pumps able to extrude arsenic from the cell cytoplasm thus lowering the intracellular concentration of the toxic ions. In Gram-negative bacteria, the efflux pump consists of a two-component ATPase complex. ArsA is the ATPase subunit and is associated with an integral membrane subunit, ArsB. Arsenate is enzymatically reduced to arsenite (the substrate of ArsB and the activator of ArsA) by the small cytoplasmic ArsC polypeptide. In Gram-positive bacteria, comparable arsB and arsC genes (and proteins) are found, but arsA is missing. In addition to the wide spread plasmid arsenic resistance determinant, a few bacteria confer resistance to arsenite with a separate determinant for enzymatic oxidation of more-toxic arsenite to less-toxic arsenate. In contrast to the detailed information on the mechanisms of arsenic resistance in bacteria, little work has been reported on this subject in algae and fungi.

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“…However, resistance to significant levels of arsenic has also been shown in laboratory strains of commonly studied bacteria, such as Escherichia coli (Cai et al 1998), Staphylococcus aureus (Ji and Silver 1992), and Pseudomonas aeruginosa (Cervantes and Chavez 1992).…”

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confidence: 99%

Arsenic-resistant Pseudomonas spp. and Bacillus sp. bacterial strains reducing As(V) to As(III), isolated from Alps soils, Italy

et al. 2011

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Five arsenic-resistant bacterial strains (designated MP1400, MP1400a, MP1400d, APSLA3, and BPSLA3) were isolated from soils collected at the Alps region (Italy), which showed no contamination by arsenic. Phylogenetic analysis of the 16S rRNA gene sequences assigned them to the genera Pseudomonas and Bacillus. Bacillus sp. strain 1400d and Pseudomonas spp. strains APSLA3 and MP1400 showed higher tolerance to As(III), as indicated by minimum inhibitory concentrations of 10 mmol/L. Pseudomonas sp. strain MP1400 exhibited higher tolerance to As(V) (minimum inhibitory concentration of 135 mmol/L). The isolated arsenic-resistant strains were able to reduce As(V) to As(III), especially Pseudomonas sp. strain MP1400 reducing 2 mmol/L of As(V) to As(III) within 24 h. The results suggest that the isolated bacterial strains play a role in the arsenic biogeochemical cycle of arsenic-poor soils in the Alps mount area.

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Plasmid-determined resistance to arsenic and antimony inPseudomonas aeruginosa

Cited by 14 publications

References 21 publications

Arsenic-resistant bacteria isolated from agricultural soils of Bangladesh and characterization of arsenate-reducing strains

Arsenic-resistant bacteria isolated from agricultural soils of Bangladesh and characterization of arsenate-reducing strains

Resistance to arsenic compounds in microorganisms

Arsenic-resistant Pseudomonas spp. and Bacillus sp. bacterial strains reducing As(V) to As(III), isolated from Alps soils, Italy

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