Plasmid Content of Isolates of Erwinia Amylovora From Orchards in Washington and Oregon in the Usa

Carey, Alyssa; Pusey, P.L.; Smith, Timothy J.; Loper, Joyce E.; Stockwell, Virginia O.

doi:10.17660/actahortic.2011.896.15

Cited by 6 publications

(2 citation statements)

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“…Such a reaction may indicate an earlier acquisition of pEA29 from E. amylovora. By contrast, a few isolates of E. amylovora without this plasmid were found in Egypt, Iran, Spain and the USA (Carey et al 2011;Llop et al 2006;Mohammadi et al 2009). Also, it was found that another plasmid of approximately 65 kb (pEI70) present in one of these isolates, showed no homology with pEA29.…”

Section: Analysis Of Nucleic Acidsmentioning

confidence: 95%

Phenotypic and genetic diversity of Erwinia amylovora: the causal agent of fire blight

Puławska

Sobiczewski

2011

Trees

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Erwinia amylovora is a polyphagous bacterium causing fire blight on apple, pear and over 130 other plant species belonging mainly to the Rosaceae family. Although E. amylovora is regarded as a very homogenous species, the particular strains can differ in pathogenic ability as far as their host range is concerned (e.g. those originating from Rubus or Maloidae plants) as well as by the extent of the disease they cause. It was found that strains originating from North America are generally more genetically heterogeneous than those from Europe. Diversity of E. amylovora is also related to streptomycin resistance as a result of its application to control of fire blight. The level of genetic heterogeneity of E. amylovora is so low (comparative genome analysis revealed a similarity of over 99% for the two genomes tested) that standard DNA-based techniques fail in detection of intra-species variability. Amplified fragment length polymorphism was found to be most useful for differentiation of strains of fire blight causal agent as well as techniques ensuing release of pangenome sequences of two E. amylovora strains: multi-locus variable number of tandem repeats analysis and clustered regularly interspaced short palindrome repeats.

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Section: Analysis Of Nucleic Acidsmentioning

confidence: 95%

Phenotypic and genetic diversity of Erwinia amylovora: the causal agent of fire blight

Puławska

Sobiczewski

2011

Trees

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“…The diversity in the pan-genome of E. amylovora is associated primarily with plasmid loss and gain. Some strains lack peA29 (Carey et al 2011;llop et al 2006;Mohammadi et al 2009), and strains with other plasmids have been isolated from orchards (reviewed by llop et al 2012).…”

Section: Introductionmentioning

confidence: 99%

A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids

et al. 2014

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Recent genome analysis of Erwinia amylovora, the causal agent of fire blight disease on Rosaceae, has shown that the chromosome is highly conserved among strains and that plasmids are the principal source of genomic diversity. A new circular plasmid, pEA68, was found in E. amylovora strain 692 (LMG 28361), isolated in Poland from Sorbus (mountain ash) with fire blight symptoms. Annotation of the 68,763-bp IncFIIa-type plasmid revealed that it contains 79 predicted CDS, among which two operons (tra, pil) are associated with mobility. The plasmid is maintained stably in E. amylovora and does not possess genes associated with antibiotic resistance or known virulence genes. Curing E. amylovora strain 692 of pEA68 did not influence its virulence in apple shoots nor amylovoran synthesis. Of 488 strains of E. amylovora from seventeen countries, pEA68 was only found in two additional strains from Belgium. Although the spread of pEA68 is currently limited to Europe, pEA68 comprises, together with pEA72 and pEA78 both found in North America, a new plasmid family that spans two continents.

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Erwinia amylovora loop-mediated isothermal amplification (LAMP) assay for rapid pathogen detection and on-site diagnosis of fire blight

Bühlmann

Pothier

Rezzonico

et al. 2013

Journal of Microbiological Methods

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Plasmid Content of Isolates of Erwinia Amylovora From Orchards in Washington and Oregon in the Usa

Cited by 6 publications

References 0 publications

Phenotypic and genetic diversity of Erwinia amylovora: the causal agent of fire blight

Phenotypic and genetic diversity of Erwinia amylovora: the causal agent of fire blight

A novel plasmid pEA68 of Erwinia amylovora and the description of a new family of plasmids

Erwinia amylovora loop-mediated isothermal amplification (LAMP) assay for rapid pathogen detection and on-site diagnosis of fire blight

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