Plasmid content and homology of 16 strains of filamentous, nonheterocystous cyanobacteria

Felkner, Roland H.; Barnum, Susan R.

doi:10.1007/bf01568817

Cited by 20 publications

(13 citation statements)

References 11 publications

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“…A method for isolating plasmid DNA from Nostoc 291 33 was necessary to perform this experiment. Several published methods specific to cyanobacteria (Felkner & Barnum, 1988 ;Potts, 1989;Stuttzman & Gendel, 1985) failed to yield usable DNA, principally because few Nostoc 291 33 cells were lysed. As with the total DNA isolation protocol, pre- liminary 5 M NaCl washes in the plasmid preparation protocol improved subsequent cell lysis.…”

Section: Nostoc 29133 Dna Isolation and Library Constructionmentioning

confidence: 99%

Transposon mutagenesis of Nostoc sp. strain ATCC 29133, a filamentous cyanobacterium with multiple cellular differentiation alternatives

et al. 1994

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Nostoc sp. strain ATCC 29133 (PCC 73102; Nostoc 29133) is a symbioticallycompetent, facultatively heterotrophic, diazotrophic cyanobacterium with the capacity t o differentiate specialized cells such as heterocysts, akinetes and hormogonial filaments. We have optimized several methods for physiological and molecular genetic analysis of Nostoc 291 33. By use of a Tn5 derivative, Tn5-1063 (Kmr Bmr Smr), delivered by conjugation from Escherichia coli, antibiotic-resistant mutants of Nostoc 291 33 were generated at a frequency of approximately 1 x (heterocyst) defective phenotype. Mutant strain UCD 328 was isolated after coculture of 86 Nostoc 29133: :Tn5-1063 clones with the symbiotic plant partner, Anthoceros punctatus; strain UCD 328 expressed a symbiotic phenotype of increased frequency of hormogonia-dependent infection. The transposon and flanking genomic DNA was recovered from strain UCD 328, the mutation and phenotype reconstructed by homologous recombination in Nostoc 291 33, and the transposition site identified from a Nostoc 29133 genomic library. Transposon mutagenesis has thus provided the means for isolation and identification of developmental and symbiotic-specific genes of Nostoc 29133. 04% of which expressed a nitrogen fixation

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Section: Nostoc 29133 Dna Isolation and Library Constructionmentioning

confidence: 99%

Transposon mutagenesis of Nostoc sp. strain ATCC 29133, a filamentous cyanobacterium with multiple cellular differentiation alternatives

et al. 1994

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“…These similarities and their prokaryotic nature make cyanobacteria good model systems for molecular genetic studies of chloroplasts. Many cyanobacterial strains contain endogenous plasmids of various sizes (2,6,8,28,29,31,39,40,43,(46)(47)(48)52), but thus far all of these plasmids have proven to be cryptic.Central to the genetic study of cyanobacteria has been the development of efficient cloning vectors that can transform cyanobacteria. However, the construction of these cloning and expression vectors is greatly dependent upon an understanding of DNA replication of cyanobacterial plasmids.…”

mentioning

confidence: 99%

A small plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC 6803 encodes a rep protein and replicates by a rolling circle mechanism

Yang

McFadden

1993

J Bacteriol

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Cyanobacteria are gram-negative prokaryotes that carry out oxygenic photosynthesis. In their function and ultrastructure, they have numerous similarities to chloroplasts in higher plants (19). These similarities and their prokaryotic nature make cyanobacteria good model systems for molecular genetic studies of chloroplasts. Many cyanobacterial strains contain endogenous plasmids of various sizes (2,6,8,28,29,31,39,40,43,(46)(47)(48)52), but thus far all of these plasmids have proven to be cryptic.Central to the genetic study of cyanobacteria has been the development of efficient cloning vectors that can transform cyanobacteria. However, the construction of these cloning and expression vectors is greatly dependent upon an understanding of DNA replication of cyanobacterial plasmids. At present, no cyanobacterial plasmids are found to replicate in Escherichia coli (27, 53), and no E. coli plasmids except a promiscuous IncQ plasmid (24) are known to replicate within cyanobacteria (26,45,58). Therefore, many shuttle vectors comprising E. coli plasmid and cyanobacterial DNA have been constructed (4, 9-13, 16, 22, 27, 30). However, these chimeric plasmids usually do not replicate efficiently in cyanobacteria (6, 45, 55), leading to difficulties in identifying these plasmids directly and employing them as efficient expression vectors in cyanobacteria. It appears that the copy numbers of the plasmids in cyanobacteria are stringently controlled. Furthermore, in many cases, shuttle vectors tend to integrate into chromosomal DNA and recombine with resident plasmids (26,49,55,57 been established that most plasmids from gram-negative bacteria (for example, F, pSC101, R6K, R1, and ColEl-type plasmids) replicate by a theta-form mechanism. In contrast, plasmids of less than 10 kbp from gram-positive bacteria, such as the pT181 and pUB110 families, replicate by a rolling circle mechanism via a single-stranded (ss) intermediate (17,25) and therefore are referred to as ssDNA plasmids (17). One exception is the recent discovery that plasmid pKYM from the gram-negative bacterium Shigella sonnei replicates by a rolling circle instead of a theta-form mechanism (59).With the exception of ColEl-type plasmids (23), most of the characterized plasmids encode a specific replication (Rep) protein which, in addition to the host proteins, is necessary for their replication (17,44). In contrast to the situation with E. coli, studies of the replication of cyanobacterial plasmids at the molecular level have never been performed. To enhance the understanding of the replication, genomic organization, and function of cyanobacterial plasmids, we report here the complete nucleotide sequence of a small plasmid, pCA2.4, isolated from the unicellular cyanobacterium Synechocystis strain PCC 6803. This plasmid contains an open reading frame (ORF) which, on the basis of the homology studies, probably encodes a replication protein (RepA). This putative RepA protein has been expressed in E. coli, and the gene product has been characterized. We also report the ...

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“…Analysis of unicellular Cynobacteria Synechococcus strains has revealed to carry homologous plasmids. Even genetically distinct group of strains carry identical plasmids which may be due to intergeneric or interspecies transmission of plasmids in nature [66]. But most of their function is still unknown.…”

Section: Properties Of Plasmidmentioning

confidence: 99%

Role of Plasmids in Microbiology

Banu¹,

Prasad²

2017

J Aquac Res Development

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Plasmid content and homology of 16 strains of filamentous, nonheterocystous cyanobacteria

Cited by 20 publications

References 11 publications

Transposon mutagenesis of Nostoc sp. strain ATCC 29133, a filamentous cyanobacterium with multiple cellular differentiation alternatives

Transposon mutagenesis of Nostoc sp. strain ATCC 29133, a filamentous cyanobacterium with multiple cellular differentiation alternatives

A small plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC 6803 encodes a rep protein and replicates by a rolling circle mechanism

Role of Plasmids in Microbiology

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