Plasmatic Membrane Expression of Adhesion Molecules in Human Cardiac Progenitor/Stem Cells Might Explain Their Superior Cell Engraftment after Cell Transplantation

Abstract

Human bone marrow mesenchymal stem cells (BM-MSCs) and cardiac progenitor/stem cells (CPCs) have been extensively studied as a potential therapeutic treatment for myocardial infarction (MI). Previous reports suggest that lower doses of CPCs are needed to improve cardiac function relative to their bone marrow counterparts. Here, we confirmed this observations and investigated the surface protein expression profile that might explain this effect. Myocardial infarction was performed in nude rats by permanent ligation of the left coronary artery. Cardiac function and infarct size before and after cell transplantation were evaluated by echocardiography and morphometry, respectively. The CPC and BM-MSC receptome were analyzed by proteomic analysis of biotin-labeled surface proteins. Rats transplanted with CPCs showed a greater improvement in cardiac function after MI than those transplanted with BM-MSCs, and this was associated with a smaller infarct size. Analysis of the receptome of CPCs and BM-MSCs showed that gene ontology biological processes and KEGG pathways associated with adhesion mechanisms were upregulated in CPCs compared with BM-MSCs. Moreover, the membrane protein interactome in CPCs showed a strong relationship with biological processes related to cell adhesion whereas the BM-MSCs interactome was more related to immune regulation processes. We conclude that the stronger capacity of CPCs over BM-MSCs to engraft in the infarcted area is likely linked to a more pronounced cell adhesion expression program.

Extracellular vesicles from dental pulp mesenchymal stem cells modulate macrophage phenotype during acute and chronic cardiac inflammation in athymic nude rats with myocardial infarction

“…Proteomic analysis of the MSC-EV biopsies was performed as described [ 34 ]. Briefly, 20 µg of a protein sample of each biopsy was treated with DL-dithiothreitol, iodoacetamide, and trypsin.…”

Extracellular vesicles from dental pulp mesenchymal stem cells modulate macrophage phenotype during acute and chronic cardiac inflammation in athymic nude rats with myocardial infarction

“…Proteomic analysis of the MSC-EVs biopsies was performed as described (34). Briefly, 20 µg of protein sample of each biopsy was treated with DL-dithiothreitol, iodoacetamide and trypsin.…”

Pre-clinical and In-silico Analysis of the Augmentation of Dermal Regeneration by Punica granatum Linn Fruit Peel in Rats

“…Then compound energy was minimized in PyRX software and converted to pdbqt format. [33][34][35][36] Protein-ligand docking Prepared protein target as well as bioactive compounds have been docked by autodock vina at PyRX version 0.8. The grid box size was kept at maximum with binding mode at 8 exhaustiveness.…”