Plasmatic Level of Leukocyte-Derived Microparticles Is Associated With Unstable Plaque in Asymptomatic Patients With High-Grade Carotid Stenosis

Sarlon-Bartoli, G.; Bennis, Youssef; Lacroix, Romaric; Piercecchi-Marti, M.D.; Bartoli, Michel; Arnaud, Laurent; Mancini, Julien; Boudes, A.; Sarlon, Emmanuelle; Thevenin, Benjamin; Leroyer, Aurélie S.; Squarcioni, C.; Magnan, Pierre Édouard; Dignat-George, Françoise; Sabatier, Florence

doi:10.1016/j.jacc.2013.03.078

Cited by 100 publications

(73 citation statements)

References 18 publications

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“…[8][9][10][11][12][13][14] Significant increases in plasma leukocyte-derived microparticles levels are associated with unstable plaques. 15 Moreover, several reports demonstrate that plasma EMP level is an novel biomarker of endothelial dysfunction, which is associated with clinical outcomes in several cardiometabolic diseases, including coronary artery disease, chronic renal failure, diabetes mellitus, and obesity. [16][17][18][19][20] In heart failure and valvular disease EMP, platelet microparticle and leukocyte microparticle levels are increased.…”

Section: Extracellular Microvesicles As Biomarkersmentioning

confidence: 99%

Microvesicles as Cell–Cell Messengers in Cardiovascular Diseases

Loyer

Vion

Tedgui

et al. 2014

Circulation Research

360

299

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Section: Extracellular Microvesicles As Biomarkersmentioning

confidence: 99%

Microvesicles as Cell–Cell Messengers in Cardiovascular Diseases

Loyer

Vion

Tedgui

et al. 2014

Circulation Research

360

299

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“…In fact EMPs may contribute to thrombosis in animal models [59]. In this context, EMPs are discussed as a promising biomarker associated with plaque vulnerability in patients with high-grade carotid stenosis [60]. Moreover, it is known that increased microparticle shedding from EPCs may reduce circulating EPC levels and may thus contribute to increase aortic stiffness beside traditional risk factors and Framingham risk [11].…”

Section: Age-related Metabolic and Vascular Diseases And Endothelial-mentioning

confidence: 99%

Predictive role of circulating endothelial-derived microparticles in cardiovascular diseases

Berezin

Zulli

Kerrigan

et al. 2015

Clinical Biochemistry

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“…Plasmatic levels of LMVs have been associated with unstable plaques in asymptomatic patients with high-grade carotid stenosis [13] or with progression of diabetic retinopathy [14]. LMVs are ambivalent bioactive structures affecting both proinflammatory [15,16] and anti-inflammatory processes [17].…”

Section: Introductionmentioning

confidence: 99%

A new assay to evaluate microvesicle plasmin generation capacity: validation in disease with fibrinolysis imbalance

Cointe

Souab

Bouriche³

et al. 2018

J of Extracellular Vesicle

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Among extracellular vesicles, leukocyte-derived microvesicles (LMVs) have emerged as complex vesicular structures. Primarily identified as procoagulant entities, they were more recently ascribed to plasmin generation capacity (MV-PGC). The objectives of this work were (1) to develop a new hybrid bio-assay combining the specific isolation of LMVs and measurement of their PGC, and compare its performance to the original method based on centrifugation, (2) to validate MV-PGC in septic shock, combining increased levels of LMVs and fibrinolytic imbalance. Using plasma sample spiked with LMVs featuring different levels of PGC, we demonstrated that CD15-beads specifically extracted LMVs. The MV dependency of the test was demonstrated using electron microscopy, high speed centrifugation, nanofiltration and detergent-mediated solubilization and the MV-PGC specificity using plasmin-specific inhibitors, or antibodies blocking elastase or uPA. Thanks to a reaction booster (ε-ACA), we showed that the assay was more sensitive and reproducible than the original method. Moreover, it exhibited a good repeatability, inter-operator and inter-experiment reproducibility. The new immunomagnetic bio-assay was further validated in patients with septic shock. As a result, we showed that MV-PGC values were significantly lower in septic shock patients who died compared to patients who survived, both at inclusion and 24 h later (1.4 [0.8–3.0] vs 3.1 [1.7–18] A405 × 10−3/min, p = 0.02; 1.4 [1–1.6] vs 5.2 [2.2–16] A405 × 10−3/min, p = 0.004). Interestingly, combining both MV-PGC and PAI-1 in a ratio significantly improved the predictive value of PAI-1. This strategy, a hybrid capture bioassay to specifically measure LMV-PGC using for the first time, opens new perspectives for measuring subcellular fibrinolytic potential in clinical settings with fibrinolytic imbalance.

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Plasmatic Level of Leukocyte-Derived Microparticles Is Associated With Unstable Plaque in Asymptomatic Patients With High-Grade Carotid Stenosis

Abstract: LMP constitute a promising biomarker associated with plaque vulnerability in patients with high-grade carotid stenosis. These data provide clues for identifying asymptomatic subjects that are most at risk of neurologic events.

Cited by 100 publications

References 18 publications

Microvesicles as Cell–Cell Messengers in Cardiovascular Diseases

Microvesicles as Cell–Cell Messengers in Cardiovascular Diseases

Predictive role of circulating endothelial-derived microparticles in cardiovascular diseases

A new assay to evaluate microvesicle plasmin generation capacity: validation in disease with fibrinolysis imbalance

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