Plasma Vitellogenin Levels during the Annual Reproductive Cycle of the Female Rainbow Trout (Oncorhynchus mykiss): Establishment and Validation of an ELISA

The influence of 17β-estradiol on intestinal calcium carbonate precipitation and osmoregulation in seawater-acclimated rainbow trout (Oncorhynchus mykiss)

“…Plasma vitellogenin was therefore measured in the present study as an indicator of the biological response to the E2 implantation treatments and their estrogenic stimulation. The concentrations of vitellogenin in the control group corresponded to those expected in female rainbow trout during early gonadal development (Bon et al, 1997). Elevated levels of plasma vitellogenin were clearly observed in a dose-dependent manner for the low and high E2 treatments (Fig.1).…”

“…Elevated levels of plasma vitellogenin were clearly observed in a dose-dependent manner for the low and high E2 treatments (Fig.1). For females injected with the low dose and high dose of E2, the concentrations of vitellogenin measured in the plasma correspond with those of females undergoing early and late vitellogenesis during the reproductive cycle (Bon et al, 1997).…”

Evaluation of estrogenic activities and mechanism of action of perfluorinated chemicals determined by vitellogenin induction in primary cultured tilapia hepatocytes

“…A non-competitive ELISA was employed to determine hepatocelluar VTG production using a polyclonal rabbit antibody against tilapia prepared in our laboratory as described by Bon et al (1997) with modifications. Briefly, VTG was induced by intraperitoneal (i.p.)…”

Development of an enzyme‐linked immunosorbent assay for quantifying vitellogenin in Pacific salmon and assessment of field exposure to environmental estrogens

“…This assay has a sensitivity range of 9 to 313 ng/ml VTG (80-20% binding), which is similar to that of VTG ELISAs for other salmonids such as rainbow trout, brown trout, and brook trout [14,[20][21][22] and other teleosts, such as common sole, zebrafish, and English sole [13,16,23].…”

“…Why plasma versus serum would behave differently in this respect is not clear. However, other investigators have also reported assay interference or inhibition in various VTG ELISAs [13,14,20,21,23], usually necessitating plasma dilutions of 1:10 to 1:100 for accurate VTG measurement.…”