Plasma Vascular Endothelial Growth Factor Concentrations in Healthy Dogs and Dogs with Hemangiosarcoma

Clifford, Craig; Hughes, Dez; Beal, Matthew W.; Mackin, Andrew; Henry, Carolyn J.; Shofer, Frances S.; Sørenmo, Karin U.

doi:10.1111/j.1939-1676.2001.tb01244.x

Cited by 78 publications

(67 citation statements)

References 35 publications

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“…[26][27][28] Platelets and leukocytes have been demonstrated to store and release other angiogenic factors such as VEGF. 26,29 The use of platelet poor plasma has been advocated when assaying angiogenic factors to reduce errors associated with thrombocytopenia or sample handling. 28 A recent study did not find an association of leukocyte and platelet counts with plasma VEGF concentrations in dogs with HSA.…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that endostatin is stored and released by activated platelets, systemic or localized consumptive coagulopathies, or immune-mediated platelet destruction, which are associated with HSA, and may be responsible for increases in endostatin seen in the diseased dogs in this study. 29 Attempts to correlate platelet counts with endostatin concentrations were not made because platelet counts were not performed in all healthy dogs, were not available from the medical records of all client-owned dogs, or were unable to be accurately reported because of sample handling or analytic errors. However, in dogs with complete laboratory evaluations, supportive evidence of disseminated intravascular coagulation was present in 4 of 12 (25%) dogs with HSA.…”

Section: Discussionmentioning

confidence: 99%

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Endostatin Concentrations in Healthy Dogs and Dogs with Selected Neoplasms

Rossmeisl

Bright

Tamarkin

et al. 2002

Veterinary Internal Medicne

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Endostatin prevents angiogenesis and tumor growth by inhibiting endothelial cell proliferation and migration. The purpose of this study was to determine serum endostatin concentrations in 53 healthy dogs and in 38 dogs with confirmed malignant neoplasms. Endostatin concentration was determined with a competitive enzymatic immunoassay (EIA) with rabbit polyclonal antibody generated against a recombinant canine endostatin protein. Both the presence of cancer and increasing age were associated with increased serum concentration of endostatin. Endostatin concentration in healthy dogs was 87.7 Ϯ 3.5 ng/mL. Upper and lower limits of the reference range for serum endostatin concentration in healthy dogs were 60 and 113 ng/mL. Dogs with lymphoma (LSA) and hemangiosarcoma (HSA) had endostatin concentrations of 107 Ϯ 9.3 ng/mL. In conclusion, this study demonstrates that endostatin can be quantified in dogs and that endostatin concentrations are high in dogs with HSA and LSA.Key words: Angiogenesis; Neoplasia; Vascular biology.A ngiogenesis plays an integral role in a variety of normal physiologic states and has been recognized for its role in the pathogenesis of diseases such as atherosclerosis and neoplasia. 1,2 Biologic tumor behavior is linked to the ability of neoplasms to induce endothelial cell growth and organization, with tumor growth being dependent on regulation of angiogenesis. [3][4][5][6] With in vivo and in vitro models, researchers have shown that without recruitment of a new blood supply, tumor size is limited to a few cubic millimeters because of a lack of energy substrate delivery, and expression of the metastatic phenotype is delayed or absent. 3,5 Alternatively, aggressive malignancies with high distant metastatic potential suppress anti-angiogenic factors, stimulate local production of angiogenic substances by normal host tissues, and endogenously produce active angiogenic compounds. 2,4,7 Endostatin, a carboxy-terminal fragment of collagen XVIII originally isolated from a murine hemangioendothelioma cell line, inhibits angiogenesis by preventing endothelial proliferation and migration. 3,5 Anti-neoplastic properties of endostatin have been demonstrated in vitro and in several animal models with multiple neoplastic cell lineages, and increased circulating endostatin concentrations may be associated with the development or recurrence of neoplasia in mice and humans. 3,5,[8][9][10] Hemangiosarcoma (HSA) is an aggressive malignancy primarily affecting older male, large-breed dogs. 11-17 HSA typically affects the spleen, right atrium, and skin or sub-

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Endostatin Concentrations in Healthy Dogs and Dogs with Selected Neoplasms

Rossmeisl

Bright

Tamarkin

et al. 2002

Veterinary Internal Medicne

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“…VEGF levels were also studied in dogs suffering from haemangiosarcoma. Elevated levels were reported in dogs diagnosed with tumours, although no correlation was observed in terms of advancement of the disease or tumour size (4). Similar results were reported by Wergin and Kaser-Holtz (23), who demonstrated that the level of VEGF were undetectable in healthy dogs, while in dogs suffering from tumours, the obtained results were statistically significant (P = 0.008) and were within the range of 7.43 ± 11.2 pg/mL.…”

Section: Introductionmentioning

confidence: 91%

Vascular endothelial growth factor in prognosis of splenic malignant tumours in dogs

Sobczyńska-Rak

Polkowska

Brodzki

2014

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to determine the levels of the vascular endothelial growth factor (VEGF) in the serum of dogs suffering from splenic malignant tumours, prior to splenectomy, as well as three and six months after the surgery. Tumours and blood samples were collected from 10 dogs of various breeds, aged between 7 and 13 years, and from 10 control animals. Tumour sections were fixed in 10% buffered formalin for 24 h. The type of tumour was determined according to the WHO classification. Blood samples were centrifuged and the obtained sera were subjected to immunoenzymatic assays to determine the VEGF levels. The median of VEGF levels in the serum of dogs suffering from splenic malignant tumours was 37.85 pg/mL (15.40-107.18 pg/mL). The highest values were observed in dogs with confirmed metastases (107.18 pg/mL and 65.43 pg/mL). The VEGF values in control group were between 0.1 pg/mL and 13.04 pg/mL. A comparative analysis of the VEGF levels against the animals' survival time indicated that VEGF overexpression may serve as a prognostic factor in cases of malignant tumours of the spleen.

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“…This ELISA has already been proven reliable for plasma VEGF evaluations in dogs in recent studies [6,7,27]. The VEGF ELISA was accomplished using the protocol from the manufacturer.…”

Section: Blood Samples and Vegf Assaymentioning

confidence: 99%