Plasma Tumor Necrosis Factor-alpha (TNF-α) Levels Correlate with Disease Severity in Spastic Diplegia, Triplegia, and Quadriplegia in Children with Cerebral Palsy

Wu, Jing; Li, Xueming

doi:10.12659/msm.895400

Cited by 15 publications

(35 citation statements)

References 38 publications

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“…Some studies have shown that the l IL-6 and TNF-α level in circulating blood were very low under normal circumstances, and can be significantly increased in inflammation (Ma et al, 2014). The levels of inflammatory factors such as IL-6 and TNF-α reflected the severity of inflammatory damage and were related to the severity of disease (Wu & Li 2015). The results of the present study are consistent with the previous studies.…”

Section: Discussionsupporting

confidence: 92%

miR-19a-3p inhibition alleviates sepsis-induced lung injury via enhancing USP13 expression

Ren

Mu²,

2021

Acta Biochim Pol

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Sepsis is a systemic inflammatory response syndrome caused by various pathogenic microorganisms or toxins. Lung damage is one of the causes of death in patients with sepsis. This study aimed to investigate the role of miR-19a-3p and its regulation mechanism in sepsis-induced lung injury. MH-S cells were treated with lipopolysaccharide (LPS) to establish sepsis-induced lung injury cell model. C57BL/6 mice were injected with miR-19a-3p antagomiR and LPS to construct animal model. LPS-treated and control cells were transfected with miR-19a-3p mimic, miR-19a-3p inhibitor or USP13 expression vector . The expression levels of miR-19a-3p and USP13 were examined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. The concentration of inflammatory cytokines was measured with enzyme-linked immunosorbent assay (ELISA). The relationship of miR-19a-3p and USP13 was validated using dual-luciferase reporter assay. The lung damage was assessed with hematoxylin-eosin staining (HE). The results showed that LPS treatment increased the concentration of TNF-α, IL-6 and IL-1β in MH-S cells. In LPS treated MH-S cells, the level of miR-19a-3p gradually increased over time. Both miR-19a-3p knockdown and USP13 overexpression in MH-S cells inhibited the LPS-induced production of TNF-α, IL-6 and IL-1β. Moreover, miR-19a-3p negatively regulated the expression of USP13 in MH-S cells. Furthermore, miR-19a-3p inhibitor suppressed lung damage in sepsis model mice. In conclusion, miR-19a-3p knockdown could alleviate sepsis-induced lung injury through enhancing USP13 expression.

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Section: Discussionsupporting

confidence: 92%

miR-19a-3p inhibition alleviates sepsis-induced lung injury via enhancing USP13 expression

Ren

Mu²,

2021

Acta Biochim Pol

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“…1 A, Table 1 ). Still, we found this way of interpretating our data to be more reasonable because it allowed us to compare groups of children with different age structures and to reproduce the results from a previous study ( Wu and Li, 2015 ). We also found 1.5-fold higher levels of IL-6 in children with CP after adjusting for age ( Table 1 ), which was similar to Pingel et al.…”

Section: Resultssupporting

confidence: 56%

“…A prior sample size was calculated to justify the number of subjects needed for the investigation based on earlier research. Because Wu and Li (2015) reported that the plasma TNF-a level in the group of 54 CP subjects was 21.4 ± 6.2 pg/ml and in the group of 28 healthy controls was 11.2 ± 4.1 pg/ml, the present study would require a sample size of 6 for both groups to achieve a power of 80% and a level of significance of 5% (two sided). The sample size analysis was performed using G∗Power software (version 3.1.9.4) ( Faul et al., 2009 ).…”

Section: Methodsmentioning

confidence: 98%

“…Very little is known about the extent to which the immune system is engaged in patients with CP, but it seems that these children carry genes that up-regulate the production of pro-inflammatory cytokines in response to various unfavorable factors such as intra-amniotic infection and preterm birth ( Gibson et al., 2006 ). Moreover, Wu and Li (2015) found 2-fold increased levels of pro-inflammatory TNF-a in children with CP and suggested that inflammation may persist later in life reflecting the reconstruction of cerebral function after birth. There are also reports of increased IL-6 levels in patients with CP ( Bi et al., 2014 ; Pingel et al., 2019 ).…”

Section: Introductionmentioning

confidence: 96%

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Immunological effects of cerebral palsy and rehabilitation exercises in children

Шарова

Smiyan

Borén

2021

Brain, Behavior, & Immunity - Health

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Cerebral palsy (CP) is a group of motor disorders caused by non-progressive lesions of the premature brain with lifelong pathophysiological consequences that include dysregulation of innate immunity. Persistent inflammation with increased levels of circulating pro-inflammatory tumor necrosis factor alpha (TNF-a) is negatively associated with rehabilitation outcome in children with CP. Because of the crosstalk between innate and adaptive immunity, we investigated the effect of CP and rehabilitation exercises on the adaptive immune system in children with CP by measuring the levels of CD3 + , CD4 + , CD8 + Т-cells, and CD22 + B-cells and the levels of immunoglobulins. Children with CP had higher levels of CD3 + , CD4 + , CD8 + Т-cells, and CD22 + B-cells compared to healthy children, and the rehabilitation exercise programs produced better outcomes in terms of increased gains in motor function at an earlier age. Rehabilitation exercises performed over a month resulted in significantly decreased levels of IgA in serum and reduced numbers of B-lymphocytes and reduced IgM levels. Our study suggests that rehabilitation programs with a focus on neuroplasticity and physical exercises in children with CP can reduce both cellular and humoral immune responses.

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“…Следовательно, хотя воспалительный ответ ослабевает с возрастом, симптомы церебрального паралича не обязательно улучшаются. Уровни TNF-α до реабилитации коррелировали с увеличением повседневной активности после реабилитации (р <0,001) [23]. Это означает, что плазменная концентрация TNF-α является предиктивным фактором эффективности реабилитационной терапии у больных ДЦП.…”

Section: биомаркеры воспаленияunclassified

Cerebral palsy biomarker

Kamilova¹,

Голота²,

Vologzhanin³

et al. 2021

Physical and rehabilitation medicine, medical rehabilitation

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Cerebral palsy is a neurological disorder that is attributed to non-progressive injury or malformation that occurred in the developing fetal or infant brain. The motor disorders in cerebral palsy are often accompanied by disturbances of sensation, perception, cognition, communication, behaviour, and by epilepsy. Cerebral palsy is a complex disorder that is likely to be of multifactorial origin. Epidemiological studies have shown that the origins of most CP are prior to labor. A number of clinical risk factors for cerebral palsy have been described in the literature including preterm birth, low birth weight, inflammation, maternal infection during pregnancy and placenta pathology. Hypoxia at birth may be primary or secondary to preexisting pathology, but the currently known clinical risk factors do not explain the majority of cases. Many of these risk factors may have a genetic component. Several single nucleotide polymorphisms, DNA copy number variations and epigenetic patterns increase genetic susceptibility for cerebral palsy. Whole genome sequencing and gene expression studies may extend the percentage of cases with a genetic pathway. Clinical risk factors could act as triggers for CP where there is genetic susceptibility. These new findings should refocus research about the causes of these complex and varied neurodevelopmental disorders on the search for biomarkers of the risk of cerebral palsy. Genomics, proteomics and metabolomics have huge potential for deepening our understanding of many complex diseases by identifying diagnostic and prognostic panels of biomarkers, especially in various neurological disorders, including cerebral palsy.

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Plasma Tumor Necrosis Factor-alpha (TNF-α) Levels Correlate with Disease Severity in Spastic Diplegia, Triplegia, and Quadriplegia in Children with Cerebral Palsy

Cited by 15 publications

References 38 publications

miR-19a-3p inhibition alleviates sepsis-induced lung injury via enhancing USP13 expression

miR-19a-3p inhibition alleviates sepsis-induced lung injury via enhancing USP13 expression

Immunological effects of cerebral palsy and rehabilitation exercises in children

Cerebral palsy biomarker

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