Plasma treatment of PDMS for applications of in vitro motility assays

Lycans, Rebecca M.; Higgins, Catherine; Tanner, Michael S.; Blough, Eric R.; Day, Brad

doi:10.1016/j.colsurfb.2013.11.007

Cited by 12 publications

(16 citation statements)

References 61 publications

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“…In a 20 day aged PDMS channel which has a volume of 0.03µl, the actin sliding velocity was 1.81 µm/s and fraction of moving actin filaments was 70-77%. A quite recent work applied a 40 min air plasma pretreatment of PDMS in vitro motility flow cell [14]. It achieved similar results with a much larger microchannel (~1.2 µl), more specialized instruments (i.e., spin coater and air plasma cleaner), and less wait time.…”

Section: Resultsmentioning

confidence: 84%

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Uncured PDMS Inhibits Myosin In Vitro Motility in a Microfluidic Flow Cell

Wang

Burghardt

2018

Preprint

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Mayo Clinic Rochester Rochester, MN 55905 Subject Category: Enzymatic assays and analysis 2 ABSTRACT The myosin motor powers cardiac contraction and is frequently implicated in hereditary heart disease by its mutation. Principal motor function characteristics include myosin unitary step size, duty cycle, and force-velocity relationship for translating actin under load. These characteristics are sometimes measured in vitro with a motility assay detecting fluorescent labeled actin filament gliding velocity over a planar array of surface immobilized myosin. Assay miniaturization in a polydimethylsiloxane/glass (PDMS/glass) hybrid microfluidic flow channel is an essential component to a small sample volume assay applicable to costly protein samples however the PDMS substrate dramatically inhibits myosin motility. Myosin in vitro motility in a PDMS/glass hybrid microfluidic flow cell was tested under a variety of conditions to identify and mitigate the effect of PDMS on myosin. Substantial contamination by the monomeric species in polymerized PDMS flow cells is shown to be the cause of myosin motility inhibition. Normal myosin motility recovers by either extended cell aging (~20 days) to allow more complete polymerization or by direct chemical extraction of the free monomers from the polymer substrate. PDMS flow cell aging is the low cost alternative compatible with the other PDMS and glass modifications needed for in vitro myosin motility assaying. 3 KEY WORDS Myosin in vitro motility; Nanoliter volume motility assay; Polydimethylsiloxane/glass hybrid microfluidic; Unpolymerized PDMS inhibits myosin; Curing recovers myosin motility;

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Section: Resultsmentioning

confidence: 84%

“…Better reductions on contact angle were achieved using extracted PDMS or oxygen plasma [30; 43; 44; 45; 46; 47; 48; 49]. The silica-like surface layer formed during oxidation was shown to reduce O 2 permeability of the chamber preventing motor protein oxidation [14] but also might slow down the uncured PDMS oligomers entering solution.…”

Section: Resultsmentioning

confidence: 99%

Uncured PDMS Inhibits Myosin In Vitro Motility in a Microfluidic Flow Cell

Wang

Burghardt

2018

Preprint

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“…Consequently, this procedure could reduce the amount of potential nucleation sites for AuTTF. Second, plasma treatment was found to damage the PDMS surface by creating cracks . A detailed inspection of the plasma‐treated surfaces by atomic force microscopy and scanning electron microscopy (SEM) revealed that the cracks were as deep as 18 nm and were visible all over the surface under the conditions used here (Figure S3 of the Supporting Information).…”

Section: Resultsmentioning

confidence: 88%

Formation of Single Micro‐ and Nanowires with Extreme Aspect Ratios in Microfluidic Channels

Lenz

Sebastian

Dittrich

2019

Small

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Shown here is the site‐specific formation of single extraordinarily long metal–organic micro‐ and nanowires using a microfluidic device made of poly(dimethylsiloxane) (PDMS). This approach exploits two concepts, i) the diffusion of organic precursor molecules through PDMS and ii) the use of microfluidic channels as a growth template. To initiate wire formation, metal and organic precursor solutions are filled into different supply channels that are separated by PDMS. As the precursor diffuses through PDMS, and thereby infiltrates the adjacent channel, the growth of micro‐ and nanowires starts at the side walls of this adjacent channel. The formation yields single wires with sizes ranging from several hundreds of micrometers to millimeters at diameters of 0.5–2 µm. The principles of this formation pathway are demonstrated with the reaction of tetrathiafulvalene (TTF) and gold(III) ions that yields Au‐TTF wires. The influence of various reaction parameters including the choice of solvents and the chip fabrication protocol on the reaction are evaluated. Based on these findings, a further microfluidic device design with orthogonally arranged channels is developed, and the formation of single wires in a channel‐defined pattern is demonstrated. Moreover, the possibility of pulsed precursor supply allows for advanced control over the growth of the wires.

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“…Recent research has included optimization of plasma treatment of PDMS to minimize its oxygen permeability for myosin motility assays 65 and use of a corona discharge to selectively render channels hydrophilic for continuous detection of analytes from segmented droplets. As a result, substantial research has been directed at improving or tailoring the surface chemistry of PDMS devices.…”

Section: Microfluidicsmentioning

confidence: 99%

Analytical chemistry research at primarily undergraduate institutions: training tomorrow's investigators

Kovarik

2015

Anal. Methods

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Primarily undergraduate institutions (PUIs) are a unique training ground for young scientists who work closely with faculty on their research. Researchers at PUIs face a number of challenges, including balancing research and teaching responsibilities, obtaining sufficient funding for projects, and training students in their first research experiences. Faculty at PUIs deploy a variety of strategies to address these challenges, and the resulting research provides important benefits to faculty, students, the analytical chemistry community, and society. This perspective discusses these challenges and benefits in detail.Additionally, several vignettes describe how specific faculty members have established and maintained productive research programs at PUIs, and select publications since 2009 by PUI researchers are highlighted.

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Plasma treatment of PDMS for applications of in vitro motility assays

Cited by 12 publications

References 61 publications

Uncured PDMS Inhibits Myosin In Vitro Motility in a Microfluidic Flow Cell

Uncured PDMS Inhibits Myosin In Vitro Motility in a Microfluidic Flow Cell

Formation of Single Micro‐ and Nanowires with Extreme Aspect Ratios in Microfluidic Channels

Analytical chemistry research at primarily undergraduate institutions: training tomorrow's investigators

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