Plasma transferable inhibition of BCG induced subcutaneous inflammation in human cancer

Abstract

When a small dacron sponge disc containing live BCG was implanted subcutaneously in healthy subjects it became infiltrated with leukocytes which at 24 hours mainly involved neutrophils. this mycobacterial-induced inflammatory response was markedly impaired in patients with cancer. This inhibitory effect could be reproduced by injecting the plasma of these patients into guinea-pigs bearing identical discs. The differences in inflammatory reactions in the indirect technique were less marked than in the direct test, though a parallelism between the two was seen. The fact that the plasma from cancer patients interferes with neutrophil responses in this way does not exclude the possibility that abnormal cellular functions were also operative. Since tumour growth control depends on effective inflammation these data further underline the importance in cancer patients of a defect at this level.

Influence of Bcg Administration Time on the in-Vivo Migration of Leukocytes

“…Granulocytes are present in large numbers in inflammatory reactions and their number also are increased in chronic inflammatory diseases. However, the cellular inflammatory response is depressed in animals bearing malignant tumors (1,2) and in patients with all types of malignancies studies so far (3,4). In previous work with the guinea-pig, we showed that ketoprofen administration restored the BCG-induced migration of leukocytes that was reduced by injection of plasma obtained from cancer patients (5).…”

Temporal study of carrageenan-induced PMN migration in mice

“…The migration of leukocytes was studied using the in vivo migration test developed in our laboratory [9]. Briefly, a disk of sterilized mesh material made of rayon (obtained from Ethnor, France) was implanted subcutaneously in ether-anesthetized mice.…”