Plasma Surface Modification of Polyhedral Oligomeric Silsequioxane-Poly(carbonate-urea) Urethane with Allylamine Enhances the Response and Osteogenic Differentiation of Adipose-Derived Stem Cells

Abstract

This study present amino functionalization of biocompatible polymer polyhedral oligomeric silsequioxane poly (carbonate-urea) urethane (POSS-PCU) using plasma polymerization process to induce osteogenic differentiation of adipose derived stem cells (ADSCs). Optimization of plasma polymerization process was carried out keeping cell culture application in mind. Thus, samples were rigorously tested for retention of amino groups under both dry and wet conditions. Physio-chemical characterization was carried out using ninhydrin test, X-ray photon spectroscopy, scanning electron microscopy and static water contact analysis.Results from physio chemical characterization shows that functionalization of amino group are not stable under wet condition and optimization of plasma process is required for stable bonding of amino groups to POSS-PCU polymer. Optimized samples were later tested in vitro in short and long term culture to study differentiation of ADSCs on amino modified samples. Short term cell culture shows that initial cell attachment was significantly (p<0.001) improved on amine modified samples (NH 2 -POSS-PCU) compared to unmodified POSS-PCU. NH 2 -POSS-PCU samples also facilitates osteogenic differentiation of ADSCs as confirmed by immunological staining of cells for extracellular markers such as collagen Type I, and osteopontin.Quantification of total collagen and ALP activity also shows significant (p<0.001) increase on NH 2 -POSS-PCU samples compared to unmodified POSS-PCU.A pilot study also confirms that these optimized amino modified POSS-PCU samples can further be functionalized using bone inducing peptide such as KRSR using conventional wet chemistry.This further provides an opportunity for bio-functionalization of polymer for various tissue specific applications.

Argon plasma improves the tissue integration and angiogenesis of subcutaneous implants by modifying surface chemistry and topography

“…This is in agreement with previous reports, which suggest, that PSM has no effect on the bulk properties of materials. 11,42,43 Detailed surface analysis revealed that all treatment gases significantly decreased the surface wettability, and led to an increase in topography and surface elastic modulus of the PSM scaffolds compared with unmodified scaffolds (Figure 1; P,0.05). The O 2 -treated scaffolds demonstrated an increasing roughness and surface elastic modulus as treatment time increased.…”

Chemical group-dependent plasma polymerisation preferentially directs adipose stem cell differentiation towards osteogenic or chondrogenic lineages

“…On the other hand, Wang et al found that acrylic acid with COOH functional groups supported osteogenic differentiation of rat BM-MSCs as indicated by calcium deposition and colony formation [22] . However, while we compared human ADSC behavior on both modifications, no direct comparison of the behavior of rat MSCs on COOH and NH 2 functional groups was carried out in previous studies [9] , [22] . We indeed observed increased osteogenesis of ADSCs on COOH modified scaffolds as compared to control ones, though to a significantly lesser extent than on NH 2 scaffolds.…”

“…Allylamine and acrylic acid were used to deposit —NH 2 and —COOH groups, respectively, on the nanocomposite scaffolds [8] . We also have some initial data that allylamine modification may increase osteogenic differentiation of ADSCs [9] . Here we tested the hypothesis that different modifications of the chemical groups on the surface of the nanocomposite polymer could increase adhesion of ADSCs to the nanocomposite scaffold and selectively enhance their differentiation towards bone or cartilage.…”

Design and development of low cost polyurethane biopolymer based on castor oil and glycerol for biomedical applications

“…However, higher cost of raw material and complex chemical process of synthesizing this material significantly increases its cost. Apart from this, this synthetic polymer still needs further bulk or surface [ ] modification to improve its biological properties. The ability to tailor the bulk mechanical properties of castor oil based biopolymer PU by adding various amount of glycerol thus provides a unique opportunity to produce low cost polyurethane for biomedical applications.…”

“…Polyurethanes (PU) are the most common synthetic polymers used for various medical devices and implants . They are used in the production of medical devices and implants such as catheters, heart valves, cardiovascular devices, and artificial organs . This is possible because of their excellent structural properties, elasticity, fatigue resistance, compliance and tolerance when used in the body .…”