Plasma Soluble Human Leukocyte Antigen G Levels in Asthmatic Children

Tahan, Fulya; Patıroğlu, Türkan

doi:10.1159/000095290

Cited by 35 publications

(53 citation statements)

References 35 publications

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“…However, when the analysis was restricted to atopic asthmatics, sHLA-G levels were significantly higher in patients than in healthy controls (50).…”

Section: I) Immune-mediated Disordersmentioning

confidence: 87%

Soluble HLA-G: Are they clinically relevant?

Pistoia¹,

Morandi²,

Wang³

et al. 2007

Seminars in Cancer Biology

161

182

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HLA-G is a non-classical HLA-class Ib molecule with multiple immunoregulatory properties. Its main function in physiological conditions is to abrogate maternal NK cell activity against foetal tissue and to establish immune tolerance at maternal-foetal interface. HLA-G is expressed not only as a membrane bound molecule on the surface of cells, but also as a soluble moiety in body fluids. The major isoforms of HLA-G present in serum are soluble HLA-G1 and HLA-G5 which are generated by shedding or proteolytic cleavage of the membrane bound isoform and by secretion of a soluble isoform, respectively.Here we review the data about soluble HLA-G (sHLA-G) serum levels in different pathological conditions, including immune-mediated disorders, transplantation, and malignancies. In particular, we focus on sHLA-G expression and function in human neuroblastoma, a pediatric tumor, with special emphasis on a novel potential immuno escape mechanism utilized by NB to instruct monocytes to produce and release sHLA-G. Finally, the potential clinical relevance of sHLA-G serum levels is discussed.

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“…However, when the analysis was restricted to atopic asthmatics, sHLA-G levels were significantly higher in patients than in healthy controls (50).…”

Section: I) Immune-mediated Disordersmentioning

confidence: 87%

Soluble HLA-G: Are they clinically relevant?

Pistoia¹,

Morandi²,

Wang³

et al. 2007

Seminars in Cancer Biology

161

182

View full text Add to dashboard Cite

show abstract

“…The possible functional relationship between soluble HLA-G and T cell-IL-4 production was also suggested by the presence of sHLA-G in serum of allergic patients (46,47). Allergy is a disorder characterized by an increased ability of B cells to produce IgE to certain groups of ubiquitous Ags (allergens).…”

Section: Discussionmentioning

confidence: 99%

“…IgE Ab synthesis results from the production of IL-4 by the Th2 cells, which induces the production of IgE by B cells, whereas Th1 cells produce IFN-g that suppresses IgE synthesis (48). Higher levels of sHLA-G in the serum of patients with allergic rhinitis and in allergic children with asthma compared with healthy donors have been reported (46,47). In contrast, sublingual immunotherapy, which was associated with a Th1-type response in patients with allergic rhinitis, was associated with reduced serum levels of sHLA-G (49).…”

Section: Discussionmentioning

confidence: 99%

HLA-G5 Induces IL-4 Secretion Critical for Successful Pregnancy through Differential Expression of ILT2 Receptor on Decidual CD4+ T Cells and Macrophages

Lombardelli

Aguerre-Girr

Logiodice

et al. 2013

The Journal of Immunology

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Successful pregnancy in humans has been associated with production of IL-4 by T cells at the feto–maternal interface. Soluble HLA-G5 produced by trophoblasts potentially controls the decidual T cell cytokine profile. We studied the effect of HLA-G5 on the cytokine profile of purified human macrophages and Ag-specific T cells in vitro. We demonstrated that HLA-G5 increased production of IL-12 by purified peripheral blood macrophages. Although IL-12 production by macrophages is known to induce IFN-γ production by CD4+ T cells, HLA-G5 increased production of IL-4 but not IFN-γ by CD4+ T cells after Ag presentation by macrophages. We found that this apparent paradox was due to the differential expression of the ILT2 HLA-G5 receptor on activated T cells and macrophages. This receptor was upregulated in the former and downregulated in the latter after Ag presentation and activation of both cell types. This observation was confirmed in situ, where decidual macrophages and T cells are continuously exposed to HLA-G5 produced locally and activated by trophoblast alloantigens. Freshly isolated decidua basalis macrophages expressed lower levels of ILT2 than peripheral blood macrophages from the same pregnant women. They did not spontaneously produce IL-12, whereas freshly isolated decidual CD4+ T cells expressed high levels of activation markers (CD25, HLA-DR, and CD69) as well as ILT2 and spontaneously produced IL-4 but not IFN-γ. Therefore, HLA-G5 could be responsible, at least in part, via its interaction with ILT2, for decidual T cell IL-4 production, known to be crucial for successful pregnancy.

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“…These changes include the induction of immune Tregs as well as changes in the cytokine profile [7]. Furthermore, some studies have reported changes in the immunoregulatory HLA class Ib protein HLA-G [31,32,33,34,35]. The overall objective of the present study was to investigate changes in these parameters in patients who received SIT as an allergy treatment and to compare the changes in the parameters to those observed in healthy nonatopic controls.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of HLA-G can induce a T H 2-like cytokine profile in PBMCs and trigger the production of cytokines such as IL-4 and IL-13 [30] that promote antibody isotype switching to IgE. Recent studies have shown that sHLA-G levels are significantly higher in atopic asthmatic and allergic patients compared to healthy nonatopic individuals [31,32]. Furthermore, serum levels of sHLA-G decrease after sublingual immunotherapy (SLIT), while production of the T H 1-cytokine IFN-γ increases [33].…”

Section: Introductionmentioning

confidence: 99%

Human Leukocyte Antigen-G and Regulatory T Cells during Specific Immunotherapy for Pollen Allergy

Sørensen

Johnsen

Dalgaard

et al. 2013

Int Arch Allergy Immunol

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Background: T_H2-biased immune responses are important in allergy pathogenesis. Mechanisms of allergen-specific immunotherapy (SIT) might include the induction of regulatory T cells (Tregs) and immunoglobulin (Ig) G₄ blocking antibodies, a reduction in the number of effector cells, and skewing of the cytokine profile towards a T_H1-polarized immune response. We investigated the effects of SIT on T cells, on immunomodulation of human leukocyte antigen (HLA)-G, which has been associated with allergy, on regulatory cytokine expression, and on serum allergen-specific antibody subclasses (IgE and IgG₄). Methods: Eleven birch and/or grass pollen-allergic patients and 10 healthy nonatopic controls were studied before and during SIT. Tregs, chemokine receptors, soluble HLA-G (sHLA-G), Ig-like transcript (ILT) 2, specific IgE, and IgG₄ were studied. Peripheral blood mononuclear cells (PBMCs) were stimulated with pollen extract in vitro and immune factors were evaluated. Results: During SIT, the main changes in the peripheral blood were an increase in CXCR3⁺CD4⁺CD25⁺CD127^low/- Tregs and a decrease in CCR4⁺CD4⁺CD25⁺CD127^low/- Tregs, an increase in allergen-specific IgG₄, and a decrease in sHLA-G during the first half of the treatment period. In the PBMC in vitro experiments, the following changes were observed upon allergen-stimulation: an increase in CD4⁺CD25⁺CD127^low/- Tregs and ILT2⁺CD4⁺CD25⁺CD127^low/- Tregs, an increase in IL-10 and IL-2 levels, and an increase in sHLA-G that was most pronounced at the start of SIT. Conclusions: The changes in CXCR3⁺CD4⁺CD25⁺CD127^low/- Treg, IgG₄, and sHLA-G levels in the peripheral blood and in ILT2⁺ Treg, IL-10, IL-2, and sHLA-G levels upon in vitro allergen stimulation suggest an upregulation in immunomodulatory factors and, to some degree, a shift towards T_H1 during SIT.

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Plasma Soluble Human Leukocyte Antigen G Levels in Asthmatic Children

Cited by 35 publications

References 35 publications

Soluble HLA-G: Are they clinically relevant?

Soluble HLA-G: Are they clinically relevant?

HLA-G5 Induces IL-4 Secretion Critical for Successful Pregnancy through Differential Expression of ILT2 Receptor on Decidual CD4+ T Cells and Macrophages

Human Leukocyte Antigen-G and Regulatory T Cells during Specific Immunotherapy for Pollen Allergy

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