Plasma Soluble Corin in Patients With Heart Failure

Abstract

Background-Corin is a transmembrane protease that processes natriuretic peptides in the heart. Like many membrane proteins, corin is shed from the cell surface. Methods and Results-In this study, we obtained plasma samples from healthy controls and patients with heart failure (HF) and acute myocardial infarction. Soluble corin levels in plasma were measured by an ELISA method. In healthy adults (nϭ198), plasma corin levels were 690 pg/mL (SD, 260 pg/mL). The corin levels did not differ significantly among different age groups. In patients with HF (nϭ291), plasma corin levels were significantly lower compared with that of healthy controls (365 pg/mL [SD, 259]; PϽ0.001). The reduction in plasma corin levels seemed to correlate with the severity of HF. In patients of New York Heart Association classes II, III, and IV, plasma corin levels were 450 pg/mL (SD, 281 pg/mL; nϭ69), 377 pg/mL (SD, 270 pg/mL; nϭ132), and 282 pg/mL (SD, 194 pg/mL; nϭ90), respectively (PϽ0.001 class II vs class IV; PϽ0.05 class III vs class IV). In contrast, plasma corin levels in patients with acute myocardial infarction (nϭ73) were similar to that of healthy controls (678 pg/mL [SD, 285 pg/mL]; PϾ0.05). Conclusions-Soluble corin was detected in human plasma. Plasma corin levels were reduced significantly in patients with HF but not in those with acute myocardial infarction. Our results indicate that corin deficiency may contribute to the pathogenesis of HF and that plasma corin may be used as a biomarker in the diagnosis of HF. (Circ Heart Fail. 2010;3:207-211.)

Circulating Fragments of N-Terminal Pro–B-Type Natriuretic Peptides in Plasma of Heart Failure Patients

“…The 6 NT-proBNP immunoassays are named according to the first amino acid that the capture antibody binds to on the NT-proBNP molecule and the last amino acid that the detection antibody binds to on the NT-proBNP molecule of 1-76 amino acids: NT-proBNP 1-20 (5B6 1-12 and 13G12 [13][14][15][16][17][18][19][20] ); NT-proBNP (18H5 [13][14][15][16][17][18][19][20] and 11D1 28 -45 ); NTproBNP (5B6 [1][2][3][4][5][6][7][8][9][10][11][12] and 11D1 28 -45 ); NT-proBNP 28 -76 (11D1 28 -45 and 28F8 67-76 ); NT-proBNP (18H5 [13][14][15][16][17][18][19][20] and 28F8 67-76 ); NTproBNP 1-76 (5B6 [1][2][3][4][5][6][7][8][9][10][11][12] and 28F8 67-76 ). Each NTpr...…”

New issues on measurement of B-type natriuretic peptides

“…NT-proBNP is an inactive peptide produced in an equimolar ratio together with BNP by action of some cellular or circulating proteolytic enzymes (such as corin and furin) on the precursor pro-hormone (proBNP) (Figure 2) [1,14,[24][25][26][27][28]. As a result, NT-proBNP assay is not a reliable index of the biological (natriuretic) activity of cardiac endocrine system in severe HF because the concentration of less active peptides in patients with severe HF (such as NT-proBNP and proBNP) is greatly higher than that of the active peptide BNP (Figure 1) [1,[29][30][31][32][33].…”

“…For the first time, Lewis et al [53] were able to set up an ELISA method that measures BNP in plasma without interference by proBNP. This two-site ELISA uses a specific polyclonal antibody that recognizes the amino-terminal end of BNP 1-32 and does not cross-react with proBNP, in conjunction with a C-terminal antibody, which recognizes BNP [26][27][28][29][30][31][32] . Detection by this assay requires both aminoterminus and carboxy-terminus of BNP to be intact.…”

Circulating Soluble Corin: A New Biomarker or Risk Factor for Age-Related Disorders

“…If so, circulating soluble corin is speculated to be associated with age-related disorders. Circulating corin could be measured by enzyme-linked immunosorbent (ELISA) assays [6,[8][9][10]. To date, some small sampled case-control studies have examined circulating soluble coirn and found that corin in the circulation was decreased in osteoporosis [11], acute coronary syndrome [12], and heart failure [12] but increased in pregnant hypertension [13].…”