Plasma separation PMMA device driven by capillary force controlling surface wettability

Sakamoto, Hiroaki; Hatsuda, Ranko; Miyamura, Kazuhiro; Saito, Susumu

doi:10.1049/mnl.2011.0627

Cited by 22 publications

(23 citation statements)

References 20 publications

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“…Several polymeric materials have been used to prepare device platforms, for instance, polystyrene (PS) [15], cyclic olefin copolymer (COC) [16], polymethamethylacrylate (PMMA) [17], poly(dimethylsiloxane) (PDMS) [18][19][20], and polyurethane methacrylate (PUMA) [21]. Nevertheless, PDMS has been identified as a highly promising candidate due to its transparency, flexibility, chemical resistance, hydrophobicity, and nontoxic properties.…”

Section: Introductionmentioning

confidence: 99%

Vulcanization, centrifugation, water-washing, and polymeric covering processes to optimize natural rubber membranes applied to microfluidic devices

et al. 2015

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Natural rubber microfluidic devices are based on the replication of microchannels and chambers through the casting of latex and combine the flexibility and transparency of the polymeric platform. Natural rubber is a proposed alternative material to prepare microfluidic devices, owing to the advantages of flexibility, ecofriendliness, and lower cost compared to other commonly used polymeric microfluidic materials. However, the challenges for the use of natural rubber are the leaching of compounds when it is in contact with fluids, the low stretching resistance, and the decreases of transparency rate in terms of the water absorption rate. To overcome these issues, we report the evaluation of the essential mechanical, optical, and structural properties of natural rubber for centrifuged and pre-vulcanized rubber membranes, as well as the polymeric coating over the membrane surfaces. We propose the centrifugation process for decreasing the leach composition of the natural rubber platform and vulcanization to improve the mechanical resistance of the polymeric membrane devices. The polymeric coating prevents the leaching of compounds from natural rubber membranes and water absorption without significant reduction in transparency or increase in the hydrophobicity of the surface. Once the centrifuging, vulcanization, and coating processes improve the rubber properties, this polymer will become an alternative flexible and low-cost material for microfluidic technology.

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Section: Introductionmentioning

confidence: 99%

Vulcanization, centrifugation, water-washing, and polymeric covering processes to optimize natural rubber membranes applied to microfluidic devices

et al. 2015

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“…Secondly, the volume of the extracted blood plasma, 0.1 µL, is enough to implement the TSH test and also is more than the obtained volume in the previous filter designs [33,35]. Importantly, the use of a simple design which is only made of two parts without post processing (such as previous works [37,39]) or embedding additional part for filtration [34], makes it suitable for batch manufacturing. Furthermore, the capability of the presented microdevice for separating and gathering blood plasma within more than one Page 12 of 13 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 plasma collected channel pave the way for blood panels in a single POC.…”

Section: Integration Of Plasma Separation With Analyte Detection For mentioning

confidence: 99%

“…In 2011, Dimove et al demonstrated a stand-alone microfluidic chip that extracted plasma from a 5µL droplet of blood and integrated an ELISA assay to detect biotin levels in 10 minutes [36] using sedimentation in a trench structure. Later, in 2012, Sakamoto and their colleagues proposed a capillary driven design working on the wetting properties of PMMA [37]. 3 % in volume of plasma from a 5µL of blood was achieved, but the instability of the PMMA surface modification process and RBCs clogging in the entrance of microchannel array are challenges, which should be overcome to use this approach in point-of-care (POC) manufacturing.…”

Section: Introductionmentioning

confidence: 99%

Self-driven filter-based blood plasma separator microfluidic chip for point-of-care testing

2015

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Abstract:Recently, there is a growing need for lab-on-a-chip devices in clinical analysis and diagnostics especially near the patient care. First step, in the most blood assays is plasma extraction from whole blood. This paper presents a novel high throughput blood plasma separation microfluidic chip, which with just a single droplet of undiluted human blood (~5µL) can separate (more than 0.1µL) plasma from whole blood without the need of external forces with high purity (more than 98%) and reasonable time (3 to 5 minutes). This would be the first step towards the realization of single use, self-blood test which does not require any external forces or connection to deliver and analyze a fresh whole blood sample in contrast to the conventional blood analysis which have variable waiting times. Polydimethylsiloxane (PDMS) is utilized as the base material to manufacture the microchip due to its biocompatibility and outstanding characteristics. PDMS has been modified with a strong nonionic surfactant (Silwet L-77) to achieve a hydrophilic behavior. The main advantage of this microfluidic chip design is the clogging delay on the filtration area, which results in an increased amount of extracted plasma (0.1 µL). Moreover, the plasma can be collected in one or more 10-µm-depth channels to facilitate the detection and readout of multiple blood assays. This high volume of extracted plasma is achieved; thanks to a novel design that combines the maximum pumping efficiency without disturbing the red blood cells (RBCs) trajectory by the use of different hydrodynamic principles such as constriction effect and symmetrical filtration mode. To demonstrate the microfluidic chip functionality, a novel hybrid microdevice, exhibiting the benefits of both microfluidics and lateral flow Immuno-chromatographic tests, is designed and fabricated. The performance of the presented hybrid microdevice is validated utilizing rapid detection of the thyroid stimulating hormone (TSH) within a single droplet of whole blood. ABSTRACTRecently, there is a growing need for lab-on-a-chip devices in clinical analysis and diagnostics especially near the patient care. First step, in the most blood assays is plasma extraction from whole blood. This paper presents a novel high throughput blood plasma separation microfluidic chip, which with just a single droplet of undiluted human blood (~5µL) can separate (more than 0.1µL) plasma from whole blood without the need of external forces with high purity (more than 98%) and reasonable time (3 to 5 minutes). This would be the first step towards the realization of single use, self-blood test which does not require any external forces or connection to deliver and analyze a fresh whole blood sample in contrast to the conventional blood analysis which have variable waiting times. Polydimethylsiloxane (PDMS) is utilized as the base material to manufacture the microchip due to its biocompatibility and outstanding characteristics. PDMS has been modified with a strong nonionic surfactant (Silwet L-77) to achieve a hydro...

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“…The driving force in the capillary can be calculated by the Young-Laplace equation, 21 shown below for rectangular channels,…”

Section: Introduction Time Of the Running Buffermentioning

confidence: 99%

A Novel Solution-auto-introduction Electrophoresis Microchip Based on Capillary Force

et al. 2018

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A novel solution-auto-introduction electrophoresis microchip based on capillary force aimed at improving portability is proposed in this paper. Two kinds of materials with micropores, poly(vinyl alcohol) (PVA)-sponge and nano-sponge, were employed as suction pumps that realized the introduction of a running buffer into the reservoirs. The surfaces of the microchannels in the microchips were modified by PVA to improve the moving velocity of the running buffer and the detection performance of the microchip. The introduction velocity of a running buffer in the PVA-coated microchannels was increased by two times compared with that in the native microchannels. The electrophoresis detection performance of several microchips composed of different microchannels and suction materials were evaluated comparatively. The results indicated that the surface coating of PVA can significantly improve the repeatability of the detection results by 20-40%, and the noise of the detected signals in the PVA-coated microchips is much lower than that in the native microchips. The proposed solution-auto-introduction electrophoresis microchip is a successful attempt that completely avoids the external connectors to accomplish the auto-introduction of running buffer. The solution-auto-introduction method provides a new train of thought for portable detection instruments with electrophoresis microchips in the future.

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Plasma separation PMMA device driven by capillary force controlling surface wettability

Cited by 22 publications

References 20 publications

Vulcanization, centrifugation, water-washing, and polymeric covering processes to optimize natural rubber membranes applied to microfluidic devices

Vulcanization, centrifugation, water-washing, and polymeric covering processes to optimize natural rubber membranes applied to microfluidic devices

Self-driven filter-based blood plasma separator microfluidic chip for point-of-care testing

A Novel Solution-auto-introduction Electrophoresis Microchip Based on Capillary Force

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