Plasma rico en plaquetas y su efecto en la regeneración ósea en fracturas mandibulares. Ensayo clínico controlado

Castillo-Cardiel, Guadalupe; Medina-Quintana, Ví­ctor Manuel; Lomelí-Enríquez, Mario; Medrano-Muñoz, Fabiola; Guerrero-Velázquez, Celia; Contreras-López, Carmen Karina; Irusteta-Jiménez, Leire; Michel-Espinoza, Luis Rodrigo; González-Ojeda, Alejandro

doi:10.24875/gmm.17002574

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…Clinical applications intended to functionally activate endogenous basal cells are part of an advanced strategy adopted by regenerative medicine and tissue engineering for reduction of risk. Since the 1970s, PRP has been a familiar clinical commodity, recognized for its regenerative and healing properties [28–31]. It has been shown to enhance the proliferation and differentiation of cells, primarily through a variety of growth factors secreted by platelets (i.e., PDGF-AB, TGF-β, and FGF) [32–34].…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma activates AKT signaling to promote wound healing in a mouse model of radiation-induced skin injury

et al. 2019

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Background The skin is impacted by every form of external radiation therapy. However, effective therapeutic options for severe, acute radiation-induced skin reactions are limited. Although platelet-rich plasma (PRP) is known to improve cutaneous wound healing, its effects in the context of high-dose irradiation are still poorly understood. Methods We investigated the regenerative functions of PRP by subjecting the dorsal skin of mice to local irradiation (40 Gy) and exposing HaCaT cells to gamma rays (5 Gy). The cutaneous benefits of PRP were gauged by wound size, histologic features, immunostains, western blot, and transepithelial water loss (TEWL). To assess the molecular effects of PRP on keratinocytes of healing radiation-induced wounds, we evaluated AKT signaling. Results Heightened expression of keratin 14 (K14) was documented in irradiated HaCaT cells and skin tissue, although the healing capacity of injured HaCaT cells declined. By applying PRP, this capacity was restored via augmented AKT signaling. In our mouse model, PRP use achieved the following: (1) healing of desquamated skin, acutely injured by radiation; (2) activated AKT signaling, improving migration and proliferation of K14 cells; (3) greater expression of involucrin in keratin 10 cells and sebaceous glands; and (4) reduced TEWL, strengthening the cutaneous barrier function. Conclusions Our findings indicate that PRP enhances the functions of K14 cells via AKT signaling, accelerating the regeneration of irradiated skin. These wound-healing benefits may have merit in a clinical setting.

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Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma activates AKT signaling to promote wound healing in a mouse model of radiation-induced skin injury

et al. 2019

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“…En nuestro caso clínico, dos dosis consecutivas de PRP preparadas con nuestro protocolo y conteniendo 3,4 y 4,89 x 10 9 PLT fueron aplicadas sin activar en un paciente con fractura ósea no consolidada. Se observa que el PRP provoca un aumento de la densidad ósea en el sitio de fractura a los 30 días post segunda dosis, el mismo efecto demostrado para los PRP activados con CaCl 2 o trombina autóloga, obtenidos con el método de PRGF system y GPS III, respectivamente (28,29) y aplicados en una sola dosis. De acuerdo a la clasificación DEPA, nuestro preparado corresponde al tipo BCA mientras que las formulaciones de PRGF system y GPS III se clasifican como DCA y CAD, por lo que concluimos que el ordenamiento DEPA sugerido por Magalon (8) permite uniformar y comparar características de preparación de distintos PRPs, pero no define su equivalencia terapéutica.…”

Section: Discussionunclassified

Obtención de plasma rico en plaquetas (PRP) en el Laboratorio de Terapia Celular para uso como herramienta terapéutica en medicina regenerativa

et al. 2021

Rev. Hosp. Clín. Univ. Chile (En línea)

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Platelet rich plasma (PRP) is used to speed up tissue repair. Despite its widespread use, the therapeutic application of PRP generates controversies in clinical results due to the variability in methods of obtaining the different preparations and differences between the components of different types of PRP, so it’s recommended to mention the type of platelet preparation used. In this article, we describe technical and biologics characteristics of our platelet product, and we compare them to different commercial preparations described in order to validate their clinical use. Our results determine that the preparation can be considered a platelet rich plasma with biological activity in vivo and in vitro, which supports its use as a valid therapeutic tool, alternative to products currently available in Regenerative Medicine.

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“…Nine clinical studies (64)(65)(66)(67)(68)(69)(70)(71)(72) in this systematic review investigated the clinical effect of PRP for bone fracture treatment. The risk of bias of clinical studies in this study was independently evaluated by two reviewers according to the criteria in the Cochrane Collaboration for Systematic Reviews.…”

Section: Clinical Studiesmentioning

confidence: 99%

“…Singh et al found that the intraoperative application of PRP led to a higher mean cortex to callus ratio when dealing with diaphyseal femur fracture (64). Castillo-Cardiel et al (65) and Daif et al (71) reported the local administration of PRP increased the bone density of mandibular patients. Additionally, Wei et al reported that intraoperative application of PRP led to superior results of Bohler's Angle, the crucial angle of Gissane, and length, width, and height of the calcaneal body regarding radiographic assessment at 24 months and 72 months postoperatively (72).…”

Section: Clinical Studiesmentioning

confidence: 99%

Platelet-Rich Plasma for Bone Fracture Treatment: A Systematic Review of Current Evidence in Preclinical and Clinical Studies

et al. 2021

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Background: Recently, there is an increasing interest in the therapeutic potential of platelet-rich plasma (PRP) for bone fracture treatment. Nevertheless, the effect of PRP for bone fracture treatment remains controversial and is still a matter of discussion. Therefore, we performed a systematic review to evaluate the efficacy and safety of PRP injection for treatment of bone fracture.Methods: The main bibliographic databases, including Medline, PubMed, Embase, Web of Science, and the Cochrane library, were comprehensively searched for studies focusing on the application of platelet-rich plasma (PRP) on bone fracture treatment. All relevant articles were screened for eligibility and subdivided into the preclinical and clinical studies. Data were extracted and presented systematically.Results: Finally, twenty-six in vitro preclinical studies (basic studies), nine in vivo preclinical studies (animal studies), and nine clinical studies, met the selection criteria, and were included in the present systematic review. Preclinical studies showed an overall positive effect of PRP on osteoblast-like cells in vitro and bone healing in animal models. The most used treatment for bone fracture in animal and clinical studies is fixation surgery combined with PRP injection. The clinical studies reported PRP shortened bony healing duration, and had no positive effect on improving the healing rate of closed fractures. However, the results of functional outcomes are controversial. Additionally, compared with control group, PRP would not increase the rate of postoperative wound infection.Conclusion: The present systematic review confirmed the continuing interests of PRP as an additional treatment for bone fracture. Preclinical studies highlighted the potential value of PRP as promising therapy for bone fracture. However, the preclinical evidence did not translate into a similar result in the clinical studies. In addition, types of fractures and procedures of PRP preparation are heterogeneous in enrolled studies, which might result in controversial results. Meanwhile, characteristics of PRP, such as platelet concentration, the numbers of leukocytes, still need to be determined and further research is required.

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Plasma rico en plaquetas y su efecto en la regeneración ósea en fracturas mandibulares. Ensayo clínico controlado

Abstract: Plasma rico en plaquetas y su efecto en la regeneración ósea en fracturas mandibulares. Ensayo clínico controlado

Cited by 6 publications

References 19 publications

Platelet-rich plasma activates AKT signaling to promote wound healing in a mouse model of radiation-induced skin injury

Platelet-rich plasma activates AKT signaling to promote wound healing in a mouse model of radiation-induced skin injury

Obtención de plasma rico en plaquetas (PRP) en el Laboratorio de Terapia Celular para uso como herramienta terapéutica en medicina regenerativa

Platelet-Rich Plasma for Bone Fracture Treatment: A Systematic Review of Current Evidence in Preclinical and Clinical Studies

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