Plasma proteomics analysis of adolescent idiopathic scoliosis patients revealed by Quadrupole‐Orbitrap mass spectrometry

Wang, Qi; Wang, Chi; Liu, Jiayu; Sun, Jingru; Wang, Chengbin; Zhang, Xuesong

doi:10.1002/prca.202100002

Cited by 5 publications

(4 citation statements)

References 45 publications

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“…105 Initial studies found increased levels of calmodulin in platelets of patients with AIS, and the level appeared related to the curve progression of AIS. [105][106][107] While the involvement of platelet calmodulin in AIS was unclear, it was proposed as a possible marker for AIS. Later studies have focused on the correlation between platelet calmodulin level and the progression of AIS, and the correlation between calmodulin gene polymorphism and AIS.…”

Section: Calmodulin In Aismentioning

confidence: 99%

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The role of endocrine hormones in the pathogenesis of adolescent idiopathic scoliosis

Liang

Guo

et al. 2021

The FASEB Journal

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Adolescent idiopathic scoliosis (AIS) is the most common form of spinal deformity with an incidence of 1%-4%. 1 It is seen more frequently in adolescent females than males, for example, a cohort study from Hong Kong found that during adolescence, the ratios of AIS in girls to boys ranged from 2.7 to 8.4 with increasing spinal curvature. 2 Clinically, it is characterized by three-dimensional spine deformity, and especially by scoliosis in the sagittal plane and rotation in the coronal plane. Although the pathogenesis is still unclear, several factors appear related to AIS occurrence, including genetic, 3 endocrine hormones, 4,5 biomechanics 6 and environmental. 7 The association of endocrine hormones and AIS is of particular research interest. Adolescence is a stage of body growth, when the levels of various hormones that regulate body growth and development are changing quickly. Several studies have reported that adolescents who develop AIS have abnormal levels of multiple hormones, suggesting that hormonal factors may play a role in the occurrence and development of AIS (Table 1). For example, AIS is more common in females, suggesting that AIS may be related to abnormal levels of female sex hormones

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Section: Calmodulin In Aismentioning

confidence: 99%

“…Initial studies found increased levels of calmodulin in platelets of patients with AIS, and the level appeared related to the curve progression of AIS 105‐107 . While the involvement of platelet calmodulin in AIS was unclear, it was proposed as a possible marker for AIS.…”

Section: Calmodulin In Aismentioning

confidence: 99%

The role of endocrine hormones in the pathogenesis of adolescent idiopathic scoliosis

Liang

Guo

et al. 2021

The FASEB Journal

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“…The filtrate was discarded after centrifugation at 14,000g for 10 minutes. Subsequently, the samples were treated with dithiothreitol (Sigma, USA) and iodoacetamide (Sigma, USA), followed by digestion into peptides using trypsin (Promega, USA) according to the previously published methods ( 13 ). Finally, the desiccated peptides were resuspended in 0.1% formic acid for LC-MS/MS proteome analysis.…”

Section: Methodsmentioning

confidence: 99%

Potential plasma biomarkers at low altitude for prediction of acute mountain sickness

Guo,

Wang,

et al. 2023

Front. Immunol.

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BackgroundAscending to high altitude can induce a range of physiological and molecular alterations, rendering a proportion of lowlanders unacclimatized. The prediction of acute mountain sickness (AMS) prior to ascent to high altitude remains elusive.MethodsA total of 40 participants were enrolled for our study in the discovery cohort, and plasma samples were collected from all individuals. The subjects were divided into severe AMS-susceptible (sAMS) group, moderate AMS-susceptible (mAMS) group and non-AMS group based on the Lake Louise Score (LLS) at both 5000m and 3700m. Proteomic analysis was conducted on a cohort of 40 individuals to elucidate differentially expressed proteins (DEPs) and associated pathways between AMS-susceptible group and AMS-resistant group at low altitude (1400m) and middle high-altitude (3700m). Subsequently, a validation cohort consisting of 118 individuals was enrolled. The plasma concentration of selected DEPs were quantified using ELISA. Comparative analyses of DEPs among different groups in validation cohort were performed, followed by Receiver Operating Characteristic (ROC) analysis to evaluate the predictive efficiency of DEPs for the occurrence of AMS.ResultsThe occurrence of the AMS symptoms and LLS differed significantly among the three groups in the discovery cohort (p<0.05), as well as in the validation cohort. Comparison of plasma protein profiles using GO analysis revealed that DEPs were primarily enriched in granulocyte activation, neutrophil mediated immunity, and humoral immune response. The comparison of potential biomarkers between the sAMS group and non-AMS group at low altitude revealed statistically higher levels of AAT, SAP and LTF in sAMS group (p=0.01), with a combined area under the curve(AUC) of 0.965. Compared to the mAMS group at low altitude, both SAP and LTF were found to be significantly elevated in the sAMS group, with a combined AUC of 0.887. HSP90-α and SAP exhibited statistically higher levels in the mAMS group compared to the non-AMS group at low altitude, with a combined AUC of 0.874.ConclusionInflammatory and immune related biological processes were significantly different between AMS-susceptible and AMS-resistant groups at low altitude and middle high-altitude. SAP, AAT, LTF and HSP90-α were considered as potential biomarkers at low altitude for the prediction of AMS.

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“…For example, it has been found that the levels of plasma oxoglutarate, arginine, N-acetylaspartate, and citrate were significantly increased, whereas the levels of fumarate, glutamate, L-glutamic acid, malate, and N-acetylglutamate were reduced in AIS patients [11]. Furthermore, proteome analysis has indicated that some proteins are under or overexpressed in AIS patients [12,13], e.g., fibronectin, calmodulin, and fibrinogen concentrations were significantly higher and positively correlated with the Cobb angle [14]. Disturbance in the lipid profile has also been suggested as a marker of AIS [15].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Imbalances and Bone Remodeling Agents in Adolescent Idiopathic Scoliosis: A Study in Postmenarcheal Girls

Danielewicz,

Wójciak,

Sowa

et al. 2023

IJMS

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The causes and mechanisms underlying adolescent idiopathic scoliosis (AIS) remain unclear, and the available information regarding metabolic imbalances in AIS is still insufficient. This investigation aimed to evaluate the concentrations of specific bone remodeling-related agents in postmenarcheal girls diagnosed with AIS. The study encompassed thirty-six scoliosis patients and eighteen age-matched healthy individuals assigned to the control group. The patients underwent clinical and radiological examinations to assess the degree of the spinal deformity, type of curvature, and skeletal maturity. Blood and urine samples were collected from all participants and serological markers were measured using an enzyme-linked immunosorbent assay. Our study results demonstrated that the balance of phosphate–calcium and parathormone levels seems normal in individuals with AIS. Furthermore, no statistically significant differences were observed in the content of Klotho protein, osteocalcin, osteoprotegerin, C-terminal telopeptide of type I collagen (CTX), sclerostin, and alkaline phosphatase. Nevertheless, the serum levels of vitamin D (25-OH-D) were lowered, while N-terminal propeptide of type I procollagen (PINP), and fibroblast growth factor-23 (FGF23) were increased in the AIS group, with p-values of 0.044, 0.001, and 0.022, respectively. This finding indicates the potential involvement of these factors in the progression of AIS, which necessitates further studies to uncover the fundamental mechanisms underlying idiopathic scoliosis.

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Plasma proteomics analysis of adolescent idiopathic scoliosis patients revealed by Quadrupole‐Orbitrap mass spectrometry

Cited by 5 publications

References 45 publications

The role of endocrine hormones in the pathogenesis of adolescent idiopathic scoliosis

The role of endocrine hormones in the pathogenesis of adolescent idiopathic scoliosis

Potential plasma biomarkers at low altitude for prediction of acute mountain sickness

Metabolic Imbalances and Bone Remodeling Agents in Adolescent Idiopathic Scoliosis: A Study in Postmenarcheal Girls

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