Plasma proteome profiling of high-altitude polycythemia using TMT-based quantitative proteomics approach

Wang, Zongkui; Liu, Fengjuan; Ye, Shengliang; Jiang, Peng; Yu, Xiaochuan; Xu, Jin; Du, Xi; Ma, Li; Cao, Haijun; Yuan, Chuang; Shen, Yuanzhen; Lin, Fen; Zhang, Rong; Li, Changqing

doi:10.1016/j.jprot.2018.12.031

Cited by 33 publications

(38 citation statements)

References 38 publications

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“…Previous researchers have explored how humans, pigs, dogs, and horses adapt to high-altitude hypoxia [7]. Additionally, the proteomics approach was applied to study the adaptive mechanism to hypoxia in human [8], mouse [9], and oriental river prawn [10]. However, there is limited information about the adaptation of Holstein dairy cows introduced to the high-altitude areas.…”

Section: Introductionmentioning

confidence: 99%

Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia

Kong

Zhou

Chen

et al. 2019

Animals

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Changes in the environment such as high-altitude hypoxia (HAH) high-altitude hypoxia can lead to adaptive changes in the blood system of mammals. However, there is limited information about the adaptation of Holstein dairy cows introduced to high-altitude areas. This study used 12 multiparous Holstein dairy cows (600 ± 55 kg, average three years old) exposed to HAH conditions in Nyingchi of Tibet (altitude 3000 m) and HAH-free conditions in Shenyang (altitude 50 m). The miRNA microarray analysis and iTRAQ proteomics approach (accepted as more suitable for accurate and comprehensive prediction of miRNA targets) were applied to explore the differences in the plasma proteomic and miRNA profiles in Holstein dairy cows. A total of 70 differential miRNAs (54 up-regulated, Fold change (FC) FC > 2, and 16 down-regulated, FC < 0.5) and 226 differential proteins (132 up-regulated, FC > 1.2, and 94 down-regulated, FC < 0.8) were found in the HAH-stressed group compared with the HAH-free group. Integrative analysis of proteomic and miRNA profiles demonstrated the biological processes associated with differential proteins were the immune response, complement activation, protein activation, and lipid transport. The integrative analysis of canonical pathways were most prominently associated with the APR signaling (z = 1.604), and LXR/RXR activation (z = 0.365), and FXR/RXR activation (z = 0.446) pathways. The current results indicated that Holstein dairy cows exposed to HAH could adapt to high-altitude hypoxia by up-regulating the APR, activating the LXR/RXR and FXE/RXR pathways.

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Section: Introductionmentioning

confidence: 99%

Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia

Kong

Zhou

Chen

et al. 2019

Animals

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“…The fractions were combined and then vacuum-dried. The dried samples were subsequently reconstituted and evaluated by LC-MS/MS (Thermo Fisher Scientific), and the detailed procedures of the LC-MS/MS methodology were performed as previously described (Wang et al 2017;2019). An MS 1 survey scan of 350-1800 m/z at a resolution of 70,000 was collected with an AGC target of 3.0 Â 106 and maximum injection time of 50 millisecond (ms).…”

Section: Fasp Enzymolysismentioning

confidence: 99%

Bioactive constituents of Salvia przewalskii and the molecular mechanism of its antihypoxia effects determined using quantitative proteomics

Wang

Duo

Yan

et al. 2020

Pharmaceutical Biology

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Context: Environmental hypobaric hypoxia induces several physiological or pathological responses in individuals in high-altitude regions. Salvia przewalskii Maxim (Labiatae) (SPM) is a traditional Chinese herbal medicine and has known antibacterial, antiviral, antioxidant, anti-thrombotic, and anti-depressant activities. Objective: This study examined the antihypoxia effects of SPM in vivo. Materials and methods: The dried and pulverised of SPM was extracted from root crude drug with 70% ethanol with ultrasound. Male Sprague-Dawley rats were divided into three groups (n ¼ 10): normal group, hypoxia group (altitude of 4260 m), and hypoxia þ SPM group (altitude of 4260 m, SPM of 1.0 g/ kg/day). The experiment persisted for 4 weeks. The mean pulmonary arterial pressure (mPAP), hypoxiainducible factor-1a (HIF-1a) mRNA, and lung pathology were analysed using pulmonary artery pressure recorder, quantitative polymerase chain reaction, and histopathological analysis. Moreover, the effects of SPM on lung proteomes during hypoxia were observed by a TMT-based proteomic approach. Results: Pre-treatment with SPM decreased mPAP (24.86%) and HIF-1a (31.24%), and attenuated the pathological changes in lung tissues. In addition, a total of 28 proteins were differentially expressed in lung of hypoxia þ SPM group (fold change > ± 1.2 and p < 0.05). The differentially altered proteins were primarily associated with antioxidative stress, as evidenced by the downregulated expression of Adh7, Cyp2d1, Plod2, Selenow, ND3, and Fabp1, and fructose metabolism, as evidenced by the downregulated expression of Khk and Aldob. Discussion and conclusions: These results suggested that SPM is a promising drug for antihypoxia. The mechanism of action might be related to increasing antioxidant capacity and inhibiting fructose metabolism.

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“…Quantitative proteomics analysis revealed the dynamic network of macrophage in ammatory response [8] ; analysis plasma proteome pro ling of high-altitude polycythemia [9] . The high content of umbilical cord plasma proteins and their continuous perfusion in fetal tissues can re ect the biological status of the fetus and provide diagnostic information [10] .…”

Section: Introductionmentioning

confidence: 99%

Proteins in cord blood as a new target for prevention and treatment of bronchopulmonary dysplasia: a proteomic analysis

Wang

Mao

Chen

et al. 2021

Preprint

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This study aimed to explore the proteins in cord blood that could regulate the development of neonatal bronchi and lungs, and to find a new target for the prevention and treatment of bronchopulmonary dysplasia (BPD). In this study, proteomic analysis was used to analyze the proteins in cord blood of preterm and term infants. A total of 100 differentially expressed (57 up-regulated and 43 down-regulated) proteins were identified from preterm with BPD and term infants cord blood (fold change ≥ 1.5, P value < 0.05). GO analysis revealed that the major enrichment functions of these differential proteins were multi-organism process, stimulus, immune system process growth, reproductive process, development process and antioxidant activity. The signaling pathways involved included insulin resistance, insulin signaling pathway, IL−17 signaling pathway, PI3K−Akt signaling pathway, NF−kappa B signaling pathway, glucagon signaling pathway, apoptosis, MAPK signaling pathway, as well as glycolysis/gluconeogenesis. Further protein and protein interact (PPI) analysis revealed that the interacting proteins involved were PGAM1, CNN2, HSP90AA and DAG1, which were all crucial in the development of BPD. We found that these differential proteins in cord blood might regulate bronchopulmonary development through the abovementioned signaling pathways or their interaction proteins, which may provide a new research direction for the intervention of BPD.

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Plasma proteome profiling of high-altitude polycythemia using TMT-based quantitative proteomics approach

Cited by 33 publications

References 38 publications

Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia

Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia

Bioactive constituents of Salvia przewalskii and the molecular mechanism of its antihypoxia effects determined using quantitative proteomics

Proteins in cord blood as a new target for prevention and treatment of bronchopulmonary dysplasia: a proteomic analysis

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