Plasma protein thiol oxidation and carbonyl formation in chronic renal failure

Himmelfarb, Jonathan; McMonagle, Ellen; McMenamin, Elizabeth

doi:10.1046/j.1523-1755.2000.00443.x

Cited by 237 publications

(190 citation statements)

References 51 publications

(1 reference statement)

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“…The presence of free radical-initiated reactions of side chains of amino-acid residues is indicated by DNPH-reactive carbonyl groups of proteins. 8,13,24 In our study, significant changes in the DNPH activity of plasma proteins of EH patients have been shown for the first time. Besides, we demonstrated that the concentration of protein sulphydryl groups (P-SH) in plasma, which are important chain breaking antioxidant, 10,11 is markedly reduced in individuals with EH.…”

Section: Discussionsupporting

confidence: 57%

“…11 Several investigators suggested the involvement of RCD overload and enhanced thiol groups oxidation in the pathogenesis and maintenance of human renovascular hypertension. [12][13][14] However, no information concerning oxidative damage of proteins in patients with EH has been available. Therefore, the presence of oxidative stress-induced protein alterations in EH has yet to be addressed.…”

Section: Introductionmentioning

confidence: 99%

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Byproducts of oxidative protein damage and antioxidant enzyme activities in plasma of patients with different degrees of essential hypertension

et al. 2005

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Despite evidence that essential hypertension (EH) is a state of increased oxidative stress, the data on oxidative protein modifications is lacking. Besides, the role of extracellular antioxidant enzymes in EH has not been systematically studied. Study was performed in 45 subjects with EH and 25 normotensive controls. Patients were divided into three groups according to the 2003 ESH/ESC guidelines (grade 1-3). Plasma protein reactive carbonyl derivatives (RCD) and SH-groups (as byproducts of oxidative protein damage) as well as antioxidant enzyme activities superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase were studied spectrophotometrically and correlated with blood pressure (BP). RCD levels were increased in EH patients compared to controls and correlated significantly with both systolic blood pressure (SBP) (r ¼ 0.495, Po0.01) and diastolic blood pressure (DBP) (r ¼ 0.534, Po0.01). Plasma SH-groups content was significantly lower in all patients with EH, with no correlation with BP. SOD and catalase activity in patients with grade 1 EH were similar to that of controls. Patients with grade 2 and 3 of EH had lower SOD and catalase activity. However, significant correlation with SBP and DBP was observed for catalase only (r ¼ À0.331; Po0.05 and r ¼ À0.365; Po0.05, respectively). EH patients exhibited higher plasma GPX activity compared to those in controls, and it correlated with SBP (r ¼ 0.328; Po0.05). The results presented show that increased oxidative protein damage is present in all grades of EH. In mild hypertension extracellular antioxidant enzyme activities are not decreased, suggesting they are probably not critical in early EH, but could be important in moderate to severe EH.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Byproducts of oxidative protein damage and antioxidant enzyme activities in plasma of patients with different degrees of essential hypertension

et al. 2005

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“…As oxidation proceeds, further oxidation forms products that the carbonyl assay does not detect, so that higher levels of oxidation are not recorded (27). The increased carbonylation of serum albumin found in chronic renal failure was attributed to oxidative stress (18). We suggest that high urea might be the cause of the oxidative stress.…”

Section: Proteins Are Extensively Carbonylated In Normal Mouse Renal mentioning

confidence: 80%

“…Of note, 5 mM urea is a normal plasma concentration, and the range in uremia is 20-80 mM. Thus, the level of urea normally present in blood can cause protein carbonylation, and the higher urea in renal failure can account for the increased carbonylation observed in that condition (18).…”

Section: High Urea Causes Single-strand Breaks In Dna and 8-oxoguaninementioning

confidence: 99%

High urea and NaCl carbonylate proteins in renal cells in culture andin vivo, and high urea causes 8-oxoguanine lesions in their DNA

Zhang

Dmitrieva

Park

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

150

118

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Urea and NaCl are elevated in the renal inner medulla. We now find that a high concentration of urea or NaCl increases reactive oxygen species (ROS) in mouse renal inner medullary (mIMCD3) cells in culture. Previously, high NaCl, but not high urea, was found to cause DNA double-strand breaks. We now tested whether high urea or NaCl causes oxidative damage to DNA or cellular proteins. We find that high urea increases mIMCD3 cell DNA single-strand breaks and 8-oxoguanine lesions. High NaCl does not cause de-

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“…It is now well known that the level of carbonyl groups in proteins is increased by many metabolic alterations such as aging in rats (Reznick and Packer, 1994) or humans (Fano et al, 2001) exercise (Leeuwenburgh and Heinecke, 2001), and various diseases (Himmelfarb et al, 2000;Pennathur et al, 2005). However, the effects of the diet on protein oxidation and carbonyl contents in the literature show variable results.…”

Section: Resultsmentioning

confidence: 99%

Food restriction and refeeding in lambs influence muscle antioxidant status

Savary-Auzeloux

Durand

Gruffat

et al. 2008

Animal

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Compensatory growth, a frequent phenomenon observed in ruminants due to seasonal variation in food availability, affects protein metabolism including protein oxidation. These oxidation processes may have an impact on animal health as well as on meat protein degradation during post mortem aging (ie meat maturation). Sixteen male lambs were randomly divided into four groups. One group was fed ad libitum (C) and one group was food-restricted to 60% of the intake of the C group (R). The last two groups were restricted similarly to the R group and refed either ad libitum (RAL) or similarly to the C group (pair-feeding) (RPF). Muscles samples were taken immediately after slaughter. The present study showed that the restriction/refeeding pattern had no effect on protein oxidation in the muscles studied (longissimus dorsi (LD), semitendinosus (ST) and supraspinatus (SP)). However, total antioxidant capacity decreased after food restriction (251%, 243%, P , 0.01 for ST and LD muscles, respectively) and re-increased only after ad libitum refeeding. This alteration in the total antioxidant status can partially be explained by the similar pattern of change observed in the glutathione concentration of the muscles (225%, P , 0.05 for ST muscle and NS for the other muscles). However, none of the concentrations of other water-soluble antioxidants studied (carnosine, anserine, glutathione peroxidase and superoxide dismutase) were altered during compensatory growth. This study showed that an inappropriate feeding level following a nutritional stress induced alterations in the total antioxidant status (particularly that of glutathione), which may have consequences on animal health. Other consequences of a decrease of the animal antioxidant status in vivo could be an alteration of the protein oxidation processes during meat maturation.Keywords: antioxidant status, compensatory growth, muscle, sheep IntroductionIn the muscle post mortem, some proteins are oxidized and this process has an impact on meat tenderization (Maltin et al., 2003). Increased proteases oxidation reduces the functional properties of these proteases and decreases their efficiency in the meat protein maturation process (Rowe et al., 2004a and2004b). Consequently, it can be hypothesized that some in vivo oxidation processes in the muscles may have an impact on the enzymes activities and protein structure both in vivo and post mortem (Stadtman and Levine, 2000). To counteract these oxidation processes, many reactive oxygen species (ROS) scavengers exist within the cells (Fang et al., 2002). Among these ROS, the watersoluble antioxidants (some antioxidant enzymes, peptides or vitamins soluble in water) are of major significance to counteract protein oxidation and carbonyl formation (Hipkiss et al., 1998 and.Among the various stress affecting ruminants, the seasonal variations in forage availability during extensive rearing affect protein metabolism with as a result, for example, compensatory growth (Hoch et al., 2003). The alteration of the growth pattern during com...

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Plasma protein thiol oxidation and carbonyl formation in chronic renal failure

Cited by 237 publications

References 51 publications

Byproducts of oxidative protein damage and antioxidant enzyme activities in plasma of patients with different degrees of essential hypertension

Byproducts of oxidative protein damage and antioxidant enzyme activities in plasma of patients with different degrees of essential hypertension

High urea and NaCl carbonylate proteins in renal cells in culture andin vivo, and high urea causes 8-oxoguanine lesions in their DNA

Food restriction and refeeding in lambs influence muscle antioxidant status

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