Plasma Profiles of Glucose, Insulin and Lipids in the Male WBN/Kob-&lt;i&gt;Lepr&lt;sup&gt;fa&lt;/sup&gt;&lt;/i&gt; Rat, a New Model of Type 2 Diabetes with Obesity

Self Cite

Obesity and type 2 diabetes mellitus (T2DM) are occurring at epidemic-like rates, and these epidemics appear to have emerged largely from changes in daily diet. In the present study, we compared effects of high-fat diet (HFD) and fructose-rich diet (FRD) in WBN/Kob-Leprfa (WBKDF) rats that spontaneously develop obesity, dyslipidemia and T2DM. After a 4-week feeding of each diet, WBKDF-HFD and WBKDF-FRD rats exhibited aggravated obesity and dyslipidemia compared with WBKDF rats fed standard diet (STD). In contrast, hyperglycemia developed in WBKDF-STD rats was significantly inhibited in WBKDF-FRD rats, but not in WBKDF-HFD rats. The present study demonstrated that the 4-week feeding of HFD and FRD caused diet-induced obesity with a distinct phenotype in the glucose metabolism in WBKDF rats.

supporting

confidence: 89%

mentioning

confidence: 99%

Effects of high-fat diet and fructose-rich diet on obesity, dyslipidemia and hyperglycemia in the WBN/Kob-Leprfa rat, a new model of type 2 diabetes mellitus

Namekawa

Takagi

Wakabayashi

et al. 2017

Self Cite

“…In a previous study, we demonstrated the involvement of impaired insulin secretion in the development of hyperglycemia in fa/fa rats [7]. The aim of the present study was to examine the role of insulin resistance in the pathogenesis and development of T2DM in fa/fa rats.…”

mentioning

confidence: 83%

Role of Insulin Resistance in the Pathogenesis and Development of Type 2 Diabetes in WBN/Kob-Leprfa Rats

Okuno

Kaji

Takahashi

et al. 2013

Self Cite

WBN/Kob-Leprfa (fa/fa) rats have been identified as a new animal model of type 2 diabetes (T2DM), as they are characterized by impaired pancreatic insulin secretion and severe insulin resistance. Our previous study demonstrated impaired insulin secretion and its involvement in hyperglycemia in fa/fa rats. The present study was aimed at elucidating the role of insulin resistance in the development and progression of diabetes in these animals. Troglitazone (TGZ) was used as an insulin sensitizer. Insulin resistance and insulin secretory capacity were measured by a homeostasis model assessment of insulin resistance and the area under the blood concentration–time curve for plasma insulin levels after intravenous glucose tolerance testing, respectively. The fa/fa rats exhibited marked insulin resistance between 5 and 11 weeks of age, compared with age-matched Wistar rats. The insulin secretory capacity of fa/fa rats was higher than that of Wistar rats at 5 weeks of age, but decreased by 50% between 9 and 11 weeks of age. The fa/fa rats were fed a standard diet, with or without 0.2% w/w TGZ, for 4 weeks. Treatment with TGZ significantly improved insulin resistance, hyperglycemia and hypertriglyceridemia in both prophylactic and therapeutic study groups. These results suggest that insulin resistance is markedly involved in the development and progression of T2DM in fa/fa rats.

“…However, little is known about the relationship between visceral obesity and blood coagulation. This study investigated this relationship by measuring blood coagulation times in 2 distinct visceral obesity models; the WBN/Kob-Lepr fa (fa/ fa) rat, a genetically obese model [2,7], and the C57BL/6J mouse fed a high-fat diet, a DIO model [16]. In both animal models after body weight had plateaued, blood coagulation times were compared with those of age-matched Wistar rats and lean mice fed a standard diet, respectively.…”

mentioning

confidence: 99%

Shortened Blood Coagulation Times in Genetically Obese Rats and Diet-Induced Obese Mice

Kaji

Nagakubo

Hashida

et al. 2013

Self Cite

ABSTRACT. The aim of this study was to investigate blood coagulation times in genetically obese rats and diet-induced obese (DIO) mice in order to clarify the relationship between visceral obesity and blood coagulation. WBN/Kob-Lepr fa (fa/fa) rats, a genetically obese model, exhibited a significantly shorter activated partial thromboplastin time (aPTT) and prothrombin time (PT) than age-matched Wistar rats. C57BL/6J mice fed a high-fat diet (60%), a DIO model, exhibited significantly shorter aPTT, PT and thrombin time than lean mice fed a standard diet. Higher body weight, visceral fat weight and insulin resistance were also shared by fa/fa rats and DIO mice. These results suggest that visceral obesity is related to accelerated blood coagulation in addition to disrupted metabolism of glucose and lipids. KEY WORDS: blood coagulation time, mouse, obese, rat, visceral fat.doi: 10.1292/jvms.13-0029; J. Vet. Med. Sci. 75(9): 1245-1248, 2013 The large increase in patients with metabolic syndrome which is closely correlated with visceral obesity is a problem in urgent need of a solution in developed countries, including Japan. Visceral obesity is associated with a high incidence of ischemic heart disease [4,9], and many cases are complicated by impaired glucose tolerance, hyperlipidemia and high blood pressure [6].Thrombus formation in the coronary artery with underlying arteriosclerosis is known to be a major cause of death in patients with type 2 diabetes (T2D) and dyslipidemia, which are particularly prevalent in metabolic syndrome. The blood coagulation system plays a major role in thrombus formation. However, little is known about the relationship between visceral obesity and blood coagulation. This study investigated this relationship by measuring blood coagulation times in 2 distinct visceral obesity models; the WBN/Kob-Lepr fa (fa/ fa) rat, a genetically obese model [2,7], and the C57BL/6J mouse fed a high-fat diet, a DIO model [16]. In both animal models after body weight had plateaued, blood coagulation times were compared with those of age-matched Wistar rats and lean mice fed a standard diet, respectively. Blood coagulation times measured in the present study are activated partial prothrombin time (aPTT), prothrombin time (PT) and thrombin time (TT), the most commonly used clotting time assays in mammals. aPTT, PT and TT assess the function of the intrinsic pathway, the extrinsic pathway and the common pathway, respectively [18].This study was carried out on male fa/fa rats and agematched Wistar rats (Japan SLC Inc., Hamamatsu, Japan), or male C57BL/6J mice (Charles River Laboratories Japan, Inc., Yokohama, Japan). All animals were kept in an environment at 20°C and 50 ± 5% humidity with 12 hr of light and free access to food and water. All experimental animals used in this study were handled according to the experimental animal guidelines of Azabu University. Blood glucose levels of fa/fa rats and Wistar rats were measured weekly from 6 weeks of age using blood collected from tail veins. Rats at 10 weeks ...