Plasma proANP Decreases after Meal Intake

Goetze, Jens P.

doi:10.1373/clinchem.2012.202416

Cited by 15 publications

(8 citation statements)

References 6 publications

(8 reference statements)

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“…As in our study, Goetze found reduced plasma NT-proANP levels after a high-carbohydrate meal; however, the underlying mechanism for this phenomenon was not investigated. Similar to Goetze (22), we did not observe a significant change in plasma NT-proBNP levels over the course of 6 h after ingestion of the high-carbohydrate shake. If anything, we noted a trend towards a slight increase in plasma NT-proBNP levels at 4 to 6 h after the carbohydrate challenge in both groups (p = 0.08) (Figure 2B).…”

Section: Discussionsupporting

confidence: 87%

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Acute Metabolic Influences on the Natriuretic Peptide System in Humans

Arora

Hamid

et al. 2016

Journal of the American College of Cardiology

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BACKGROUND The cardiac natriuretic peptides (NPs), atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP), have central roles in sodium and blood pressure regulation. Extracardiac factors (e.g., obesity and diabetes) influence NP production, potentially altering cardiovascular responses to volume and pressure stress. OBJECTIVES This study examined the effects of acute carbohydrate intake on the NP system in humans, and investigated underlying mechanisms. METHODS Normotensive subjects (N = 33) were given a high-carbohydrate shake. Venous blood was sampled to measure N-terminal (NT)-proANP and NT-proBNP levels. Human embryonic stem cell–derived cardiomyocytes (hESC-CMs) and HepG2 cells were treated with glucose, and expression levels of NPs and micro ribonucleic acid 425 (miR-425), a negative regulator of ANP, were examined. The role of nuclear factor kappa B (NF-κB) in the glucose-mediated effects was investigated using a NF-κB inhibitor and expression plasmids encoding NF-κB subunits. RESULTS We observed a 27% reduction in the levels of circulating NT-proANP (p < 0.001, maximal at 6 h) after carbohydrate challenge, with no effect on NT-proBNP levels in our human subjects. Glucose treatment of hESC-CMs for 6 h and 24 h increased levels of the primary transcript of miR-425 (pri-miR-425) and mature miR-425. A corresponding decrease in NPPA messenger RNA levels was also observed at both time points. Overexpression of NF-κB subunits in H9c2 cardiomyocytes increased miR-425 levels, whereas inhibition of NF-κB abrogated the glucose-mediated increase in pri-miR-425 levels in HepG2 cells. CONCLUSIONS Acute carbohydrate challenge is associated with a reduction in ANP production. The mechanism appears to involve a glucose-induced increase in the expression of miR-425, mediated by NF-κB signaling.

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Section: Discussionsupporting

confidence: 87%

“…Previously, Goetze (22) examined the effect of a carbohydrate challenge on circulating NP concentrations in 6 individuals. As in our study, Goetze found reduced plasma NT-proANP levels after a high-carbohydrate meal; however, the underlying mechanism for this phenomenon was not investigated.…”

Section: Discussionmentioning

confidence: 99%

Acute Metabolic Influences on the Natriuretic Peptide System in Humans

Arora

Hamid

et al. 2016

Journal of the American College of Cardiology

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“…The half-life of ANP is <5 min, and while the exact half-life of proANP is unknown, we have seen 50% reduction of the proANP concentration in vivo within 60 min (13). This should facilitate our ability to detect a rise in the secretion of ANP (with its synchronous and equimolar secretion of proANP).…”

Section: Discussionmentioning

confidence: 75%

Glucagon-like peptide-1: effect on pro-atrial natriuretic peptide in healthy males

Skov¹,

Holst²,

Gøtze³

et al. 2014

Endocrine Connections

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The antihypertensive actions of glucagon-like peptide-1 (GLP1) receptor agonists have been linked to the release of atrial natriuretic peptide (ANP) in mice. Whether a GLP1–ANP axis exists in humans is unknown. In this study, we examined 12 healthy young males in a randomized, controlled, double-blinded, single-day, cross-over study to evaluate the effects of a 2-h native GLP1 infusion. Plasma proANP concentrations were measured by an automated mid-region-directed proANP immunoassay and N-terminal pro B-type natriuretic peptide (BNP) on Roche Modular E170. Urine was collected for measurements of sodium excretion. Although GLP1 infusion increased the urinary sodium excretion markedly, there were no significant changes in either proANP or proBNP concentrations. When GLP1 infusion was stopped, sodium excretion declined rapidly. As proANP concentration reflects ANP secretion, our data could not confirm the existence of a GLP1–ANP axis in humans. Especially, the natriuretic effects of GLP1 seem unlikely to be mediated exclusively via ANP.

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“…Based on this observation, hyperglycemia may be the mechanism underlying decreased plasma concentrations of ANP and its precursor, proANP, observed in obesity and type 2 diabetes. In support of this, ANP secretion decreases after a meal (Arora et al, 2016, Goetze, 2013), but whether glucose per se, or in conjunction with post-prandial hormones (Jujić et al, 2016), regulates the cardiac ANP expression, remains unclear. Importantly, cardiomyocytes express receptors for both glucagon and insulin and increased plasma concentrations of both hormones are well-known features of obesity and type 2 diabetes (Brubaker and Drucker, 2002, Gammeltoft and Van Obberghen, 1986).…”

Section: Introductionmentioning

confidence: 99%

Effect of Pancreatic Hormones on pro-Atrial Natriuretic Peptide in Humans

et al. 2017

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Plasma concentrations of pro-Atrial natriuretic peptide, proANP, are decreased in obesity and diabetes. Decreased proANP concentrations have also been noted after meal intake, and recently, a glucose-mediated regulation of ANP gene expression was reported. Hence, we evaluated the effects of insulin, glucagon and glucose on plasma proANP in a series of observational and experimental studies.Six healthy men underwent seven days of bed rest. Before and after the bed rest, hyperinsulinemic euglycemic clamps with serial plasma measurements of proANP were performed. Moreover, plasma proANP was quantified in 65 individuals with normal or impaired glucose regulation. Finally, the effects of infusion-induced hyperglucagonemia were examined in ten healthy men.Bed rest decreased insulin sensitivity and plasma proANP. The decrease in proANP was not associated with insulin sensitivity and the peptide concentrations remained constant during euglycemic hyperinsulinemia and hyperglycemic hyperglucagonemia. Impaired glucose regulation was not associated with decreased proANP concentrations.Bed rest per se induces a marked decrease in plasma proANP concentrations whereas insulin resistance and impaired glucose regulation was not associated with lower proANP concentrations. Neither acute hyperinsulinemia nor hyperglucagonemia seems to affect plasma proANP. Our findings thus suggest that decreased plasma proANP concentrations occur late in the development of insulin resistance.

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Plasma proANP Decreases after Meal Intake

Cited by 15 publications

References 6 publications

Acute Metabolic Influences on the Natriuretic Peptide System in Humans

Acute Metabolic Influences on the Natriuretic Peptide System in Humans

Glucagon-like peptide-1: effect on pro-atrial natriuretic peptide in healthy males

Effect of Pancreatic Hormones on pro-Atrial Natriuretic Peptide in Humans

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