Plasma Polymerized Pyrrole Films for Biological Applications: Correlation Between Protein Adsorption Properties and Characteristics

Abstract

The present work describes the fabrication, characterization, and optimization of plasma polymerized pyrrole films, PPpy, prepared by continuous wave for application as the adhesion layers of bovine serum albumin (BSA). Plasma conditions used throughout the work produced significant differences in the chemical, physical, and electrochemical properties of the resultant PPpy films. Structural analysis and property characterizations of PPpy were carried out using Fourier transform infrared spectroscopy, X‐ray photoelectron spectroscopy, and electrochemical workstation. Surface plasmon resonance spectroscopy was used to investigate the adsorption kinetics of BSA onto PPpy films. Moreover, when immersed in solutions or after BSA adsorption, the differences of electron transfer at the interfaces between the PPpy and the electrolyte solutions were determined by electro impedance spectroscopy. While there is a clear dependence of the protein adsorption affinity on the plasma polymerization condition employed during the film preparation. The relationship between film structure, electrochemical properties, polymer stability in aqueous buffer, and protein binding affinities are discussed.

Rheological Behavior of Plasma Polymerized Iodine-Doped Polypyrrole Particles Suspended in Solutions of Bovine Serum Albumin.

“…The presence of an ionic medium should help in the processes of the adsorption/desorption of BSA on the surface of PPPy/I particles, as suggested by the literature (Zhang et al, 2011).…”

“…7, where a possible mechanism of protein adsorption onto the surface of polymer has been established after observing the formation of the monolayer; the surface exposes polar groups of BSA containing NH, OH and COOH groups, and these groups are present in the amino acids that make up the protein structure and are highly dependent on physiological pH (Ahmed et al, 2015;Fanali et al, 2012), allowing the free BSA molecule to interact with a new surface chemical diversity. Nevertheless, when increasing the thickness of the protein layer, there are packing and hydration limits (Ahmed et al, 2015;Zhang et al, 2011) that establish a dynamic equilibrium where BSA molecules are released from last layers and replaced by new BSA molecules; this phenomenon occurs while the films are exposed to higher protein concentrations than that of the surrounding medium, which still contains proteins that are capable of interacting; this equilibrium indicates that at higher protein concentrations the observed angle does not 1124 www.rmiq.org substantially increase but instead maintains an average value of 42.6±2.0° (Vogler, 2012;Zhang et al, 2010).…”

Effects of radio frequency power on the microstructures and properties of plasma polymerized polypyrrole thin films

“…Various prototypes of sensors, gauges or even implants are essentially fabricated by this technique as reported in [21][22][23][24][25][26].…”

Nanofilms Produced by Magnetron Enhanced Plasma Polymerization from Methane and Oxygen for Coating of Rigid Contact Lenses

“…The latter is defined as a high resistance against the adsorption of biomolecules at the liquid–solid interface. PEO‐like surfaces display a specific oxygen/carbon ratio and surface structure that introduce an unfavorable energy balance for protein deposition …”