Plasma polymerization‐modified bacterial polyhydroxybutyrate nanofibrillar scaffolds

Abstract

The design and the development of novel scaffold materials for tissue engineering have attracted much interest in recent years. Especially, the prepared nanofibrillar scaffold materials from biocompatible and biodegradable polymers by electrospinning are promising materials to be used in biomedical applications. In this study, we propose to produce low-cost and cell-friendly bacterial electrospun PHB polymeric scaffolds by using Alcaligenes eutrophus DSM 545 strain to PHB production. The produced PHB was characterized by Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared Spectroscopy (FTIR). Nanofibrous scaffolds were fabricated via electrospinning method that has a fiber diameter approximately 700-800 nm. To investigate cell attachment, cell growth, and antioxidant enzyme activity on positively and negatively charged PHB scaffold, PHB surface was modified by plasma polymerization technique using polyethylene glycol (PEG) and ethylenediamine (EDA). According to the results of superoxide dismutase (SOD) activity study, PEG-modified nanofibrillar scaffolds indicated more cellular resistance against oxidative stress compared to the EDA modification. As can be seen in cell proliferation results, EDA modification enhanced the cell proliferation more than PEG modification, while PEG modification is better as compared with nonmodified scaffolds. In general, through plasma polymerization technique, surface modified nanofibrillar structures are effective substrates for cell attachment and outgrowth.

Biosynthesis and characterization of polyhydroxyalkanoates produced by an extreme halophilic bacterium,Halomonas nitroreducens, isolated from hypersaline ponds

“…As noted, this spectrum is practically identical to that obtained from a commercial polyhydroxybutyrate (PHB) sample (see Fig. 6b) acquired from Goldfellow Co. and very similar to those reported in the literature (Xiao and Jiao 2011;Karahaliloglu et al 2013) for this polymer. Therefore, there is no doubt that the polymer produced by this extreme halophilic bacterium is PHB.…”

“…b) acquired from Goldfellow Co. and very similar to those reported in the literature (Xiao and Jiao ; Karahaliloglu et al . ) for this polymer. Therefore, there is no doubt that the polymer produced by this extreme halophilic bacterium is PHB.…”

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

“…Moreover, ED‐modified scaffolds could induce less cellular resistance against oxidative stress than PEG‐modified one, but the latter was still superior to PHB scaffolds. PEG and ED‐modified scaffolds reduced the growth of calcium oxalate crystals compared to PHB, therefore, both nanofibrous mats would be employed as the potential bladder tissue engineering scaffolds for long‐term uses …”

Polymers for medical and tissue engineering applications

“…Similarly, the porosity achievable with use of aqueous emulsions have also been shown to positively impact PHB scaffold performance . Another example is the use of polyethylene glycol, which has been shown to enhance the hydrophilic properties of PHB nano‐fibrillar scaffolds . Polyhydroxyalkonoates will continue to maintain significance in the field of medical polymers with the continuing progress in recombinant, purification and modification technologies.…”