Plasma polymerised nanoscale coatings of controlled thickness for efficient solid-phase presentation of growth factors

Alba‐Perez, Andres; Jayawarna, Vineetha; Childs, Peter; Dalby, Matthew J.; Salmerón‐Sánchez, Manuel

doi:10.1016/j.msec.2020.110966

Cited by 22 publications

(39 citation statements)

References 34 publications

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“…However, between 70-80% of the BMP2 was absorbed onto the FN, and we propose that this solid-state BMP2 coupled with increased RGD availability can drive synergistic signalling between RGD and BMP2, as previously reported. 19,53,54 A key finding of this study is the reduction in bacterial adhesion seen when P. aeruginosa is cocultured with MSCs on pPEA-coated nanowires. While biofilms formed swiftly across the surface of flat Ti, very little bacterial adhesion was observed on the nanowires, in both the presence or absence of the pPEA FN/BMP2 coating (Figure 5a, b).…”

Section: Discussionmentioning

confidence: 88%

“…The 3-min PEA coating (18 kJ, 67.2±11.8 nm) supported fibrillar FN over the whole surface (Figure 3e), which supports the XPS data showing that Ti cannot be detected on the 3-min PEA coated surface ( Figure 1f). These very low energies, much lower than those previously investigated for plasma PEA coatings, 53,54 were chosen to ensure that the topographical features retain their high aspect ratio. It is also interesting that the heterogeneous globular / fibrillar FN on the 90 sec pPEA coating best supports osteogenesis when applied to the 2 hr nanowires.…”

Section: Assessing Fn/bmp2 Interactions With Pea-coated Nanowiresmentioning

confidence: 99%

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PEA polymer-coated nanotopography delivers solid-state BMP2, enhances mesenchymal stem cell adhesion, prevents bacterial biofilm formation and protects cells from quorum sensing virulence factors

Damiati

Tsimbouri

Ginty

et al. 2020

Preprint

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Post-operative infection is a major complication in patients recovering from orthopaedic surgery. As such, there is a clinical need to develop biomaterials for use in regenerative surgery that can promote mesenchymal stem cell (MSC) osteospecific differentiation and that can prevent infection caused by biofilm-forming pathogens. Nanotopographical approaches to pathogen control are being identified, including in orthopaedic materials such as titanium and its alloys. These topographies use high aspect ratio nanospikes or nanowires to prevent bacterial adhesion but these features puncture adhering cells, thus also reducing MSC adhesion. Here, we use a poly(ethyl acrylate) (PEA) polymer coating on titanium nanowires to spontaneously organise fibronectin (FN) and to deliver bone morphogenetic protein 2 (BMP2) to enhance MSC adhesion and osteospecific signalling. This nanotopography when combined with the PEA coating enhanced osteogenesis and reduced adhesion of Pseudomonas aeruginosa in culture. Using a novel MSC–Pseudomonas aeruginosa co-culture, we also show that the coated nanotopographies protect MSCs from cytotoxic quorum sensing and signalling molecules. We conclude that the PEA polymer-coated nanotopography can both support MSCs and prevent pathogens from adhering to a biomaterial surface, thus protecting from biofilm formation and bacterial infection and supporting osteogenic repair.

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Section: Discussionmentioning

confidence: 88%

Section: Assessing Fn/bmp2 Interactions With Pea-coated Nanowiresmentioning

confidence: 99%

PEA polymer-coated nanotopography delivers solid-state BMP2, enhances mesenchymal stem cell adhesion, prevents bacterial biofilm formation and protects cells from quorum sensing virulence factors

Damiati

Tsimbouri

Ginty

et al. 2020

Preprint

Self Cite

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“…In vitro Enhances proliferation and osteogenesis [48][49][50][51][52][53][54][55][56][57][58][59] Short half-life [19]; toxic at 200 ng/ml [60] In vivo Faster healing and more newly formed bone tissue [48][49][50]52,54,56,59,61,62]; increased angiogenic potential and bone regeneration capacity [51] (compared to bFGF [63])…”

Section: Bmp-2mentioning

confidence: 99%

“…It enables high availability of the binding region for the transmembrane receptor integrin (FN III, 9–10 binding site) and the binding region for a GF (FN III, 12–14 binding site). Plasma-polymerized ethyl acrylate has been shown to be more suitable as a coating compared to polymerized ethyl acrylate applied by the spin coating technique, as BMP-2 is better absorbed into the FN network and thus strongly induces mesenchymal stem cell (MSC) differentiation [ 55 ]. Compared to the plasma-polymerized film coating on 3D-printed titanium implants, ion-assisted plasma polymer (IAPP) film coatings show greater bone inductive activity, which is a consequence of the higher density and stability of the reactive IAPP layer.…”

Section: Bioactive Coatings and Gfsmentioning

confidence: 99%

The Role of Growth Factors in Bioactive Coatings

Bjelić

Finšgar

2021

Pharmaceutics

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With increasing obesity and an ageing population, health complications are also on the rise, such as the need to replace a joint with an artificial one. In both humans and animals, the integration of the implant is crucial, and bioactive coatings play an important role in bone tissue engineering. Since bone tissue engineering is about designing an implant that maximally mimics natural bone and is accepted by the tissue, the search for optimal materials and therapeutic agents and their concentrations is increasing. The incorporation of growth factors (GFs) in a bioactive coating represents a novel approach in bone tissue engineering, in which osteoinduction is enhanced in order to create the optimal conditions for the bone healing process, which crucially affects implant fixation. For the application of GFs in coatings and their implementation in clinical practice, factors such as the choice of one or more GFs, their concentration, the coating material, the method of incorporation, and the implant material must be considered to achieve the desired controlled release. Therefore, the avoidance of revision surgery also depends on the success of the design of the most appropriate bioactive coating. This overview considers the integration of the most common GFs that have been investigated in in vitro and in vivo studies, as well as in human clinical trials, with the aim of applying them in bioactive coatings. An overview of the main therapeutic agents that can stimulate cells to express the GFs necessary for bone tissue development is also provided. The main objective is to present the advantages and disadvantages of the GFs that have shown promise for inclusion in bioactive coatings according to the results of numerous studies.

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“…Triaxial fibers have a better sustained-release profile than coaxial fibers. Considering that the drug-enriched core is theoretically blocked from drug release by two other protective layers, increasing the thickness of the sheath layer in core–sheath fibers can improve the sustained-release properties of drugs [ 31 ]. The preparation of coaxial structure is less difficult than that of triaxial structure.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Core–Sheath Nanofibers with Variable Shell Thickness for Modifying Curcumin Release to Achieve a Better Antibacterial Performance

et al. 2022

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The inefficient use of water-insoluble drugs is a major challenge in drug delivery systems. Core–sheath fibers with various shell thicknesses based on cellulose acetate (CA) were prepared by the modified triaxial electrospinning for the controlled and sustained release of the water-insoluble Chinese herbal active ingredient curcumin. The superficial morphology and internal structure of core–sheath fibers were optimized by increasing the flow rate of the middle working fluid. Although the prepared fibers were hydrophobic initially, the core–sheath structure endowed fibers with better water retention property than monolithic fibers. Core–sheath fibers had flatter sustained-release profiles than monolithic fibers, especially for thick shell layers, which had almost zero-order release for almost 60 h. The shell thickness and sustained release of drugs brought about a good antibacterial effect to materials. The control of flow rate during fiber preparation is directly related to the shell thickness of core–sheath fibers, and the shell thickness directly affects the controlled release of drugs. The fiber preparation strategy for the precise control of core–sheath structure in this work has remarkable potential for modifying water-insoluble drug release and improving its antibacterial performance.

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Plasma polymerised nanoscale coatings of controlled thickness for efficient solid-phase presentation of growth factors

Cited by 22 publications

References 34 publications

PEA polymer-coated nanotopography delivers solid-state BMP2, enhances mesenchymal stem cell adhesion, prevents bacterial biofilm formation and protects cells from quorum sensing virulence factors

PEA polymer-coated nanotopography delivers solid-state BMP2, enhances mesenchymal stem cell adhesion, prevents bacterial biofilm formation and protects cells from quorum sensing virulence factors

The Role of Growth Factors in Bioactive Coatings

Electrospun Core–Sheath Nanofibers with Variable Shell Thickness for Modifying Curcumin Release to Achieve a Better Antibacterial Performance

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