Plasma Polymer Coatings To Direct the Differentiation of Mouse Kidney-Derived Stem Cells into Podocyte and Proximal Tubule-like Cells

Abstract

Kidney disease is now recognized as a global health problem and is associated with increased morbidity and mortality, along with high economic costs. To develop new treatments for ameliorating kidney injury and preventing disease progression, there is a need for appropriate renal culture systems for screening novel drugs and investigating the cellular mechanisms underlying renal pathogenesis. There is a need for in vitro culture systems that promote the growth and differentiation of specialized renal cell types. In this work, we have used plasma polymerization technology to generate gradients of chemical functional groups to explore whether specific concentrations of these functional groups can direct the differentiation of mouse kidney-derived stem cells into specialized renal cell types. We found that amine-rich (−NH 2 ) allylamine-based plasma-polymerized coatings could promote differentiation into podocyte-like cells, whereas methyl-rich (CH 3 ) 1,7-octadiene-based coatings promoted differentiation into proximal tubule-like cells (PTC). Importantly, the PT-like cells generated on the substrates expressed the marker megalin and were able to endocytose albumin, indicating that the cells were functional.

Nanoparticles Surface Chemistry Influence on Protein Corona Composition and Inflammatory Responses

“…The organic precursors were selected for their capacity to produce nano-thin plasma polymer films with specific chemical functionalities and surface charge. Figure 1B shows the chemical structures of the precursors used and a schematic representation of the surface architecture of the modified particles: acrylic acid (AC) provides films rich in carboxyl acid groups, allylamine (AA) tailors the particle surface with amine groups [45], methyl oxazoline (OX) gives heterocyclic functionality [46] and 1,7-octadiene (OD) provides pure hydrocarbon coatings [30]. These different plasma functionalities were selected to epitomize the chemical compositions of amino acids in proteins (-COOH, -NH 2 and -CH 3 ) and provide different surface charges [47].…”

Single‐step deposition of hexamethyldisiloxane surface gradient coatings with a high amplitude of water contact angles over a polyethylene foil

“…[ 23 ] Frequently reported precursors for generating plasma polymers are carboxylic acid and/or amino‐functional acrylic acid, and allylamine along with apolar hydrocarbon precursors such as 1,7‐octadiene (OD), and the gradients generated are used mainly for cell studies. [ 24–26 ] In another study, the thickness gradient of glycol dimethyl ether was generated on top of the acrylic acid plasma polymer platform by using a tilted mask. The results proved that the cell attachment and morphology varied as a function of the surface chemistry gradient.…”

Sandwich-type Surface Stress Biosensor Based on Self-Assembled Gold Nanoparticles in PDMS Film for BSA Detection

“…Nephropathy is a global health problem that poses a considerable threat to human life. Microalbuminuria is the presence of human serum albumin (HSA) at concentrations exceeding 30 μg/mL and is an early signal of nephropathy. , The severity of glomerular lesions can be inferred from the HSA concentration in urine. Therefore, detection of HSA in urine is highly significant .…”