Plasma pharmacokinetics and tissue distribution of L-lysine α-oxidase from Trichoderma cf. aureoviride RIFAI VKM F- 4268D in mice

Pokrovsky, Vadim S.; Lukashev, Alexander N.; Babayeva, Gulalek; Karshieva, Saida Sh; Arinbasarova, A. Yu.; Medentzev, A. G.; Комарова, М В; Лукашева, Е. В.

doi:10.1007/s00726-020-02930-4

Cited by 6 publications

(7 citation statements)

References 24 publications

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“…Biological experiments 13,14 were carried out on guinea pigs weighing 200-250 g and CBA mice (CBA x C57BI) weighing 18-20 g, C57 Bl, SHR.The study of the immunogenic properties of L-lysine-α -oxidase was carried out as follows. Native L-lysine-α -oxidase was injected into C57BI mice five times intravenously at a dose of 35 U/kg.…”

Section: Methodsmentioning

confidence: 99%

“…L-lysine-α -oxidase activity was noted in those cases when the immune sera were added immediately before the measurement of the activity. If the enzyme was preincubated with antibodies, the inhibitory effect increased 14,15 . This was observed with contact duration of less than one hour.…”

Section: Fig 2: the Effect Of Immune Sera On The Activity Of L-lysine...mentioning

confidence: 99%

“…However, as studies have shown, the enzyme can act as a strong inhibitor of especially dangerous plant diseases [11][12][13] . Plant objects may be considered as models for the study of this enzyme 14 . This paper is a continuation of studies related to the study of new properties of L-lysine-α -oxidase, an enzyme with antiviral and antitumor effect.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of L-Lysine-A-Oxidase, A New Antitumor And Antiviral Drug Substance Synthesized By Trichoderma

Smirnova

Кузнецов

Podoprigora

et al. 2022

Int J Life Sci Pharm Res

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The trigger to the studies of L-lysine-a -oxidase is of great interest for the scientists across the world. Regarding Russian Federation, the enzyme is under examination by several laboratories. Attracted by the polyfunctionality of the enzyme, the likelihood of utilizing this substance in the creation of multiple dosage forms of different therapeutic orientation. Thus, the main aim of the study is to analyze the new characteristics of L-lysine-a -oxidase as a new antitumor and antiviral drug substance synthesized by Trichoderma. To meet the study’s aim, a biological experiments were carried out on guinea pigs weighing 200-250 g and CBA mice (CBA x C57BI) weighing 18-20 g, C57 Bl, SHR.Native L-lysine-a -oxidase was injected into C57BI mice five times intravenously at a dose of 35 U/kg. Blood was sampled every week for four weeks from the beginning of immunization (n=7), and the obtained sera of mice were analyzed by enzyme-linked immunosorbent assay according to a previously developed method. The sera were titrated in increments of 2. Tests of L-lysine-a -oxidase in mice and guinea pigs found low immunogenicity of Llysine- a -oxidase when administered in mice at a dose of 35 U/kg, or 0.8 ml of protein/kg. Based on the results obtained, antibodies not only reduce its catalytic activity but also affect the affinity of the enzyme with the substrate. Experiments on guinea pigs have shown that native and modified L-lysine-a -oxidase at a dose of 35 U/kg in vivo and in vitro has a low allergenic activity. The allergenic activity of L-lysine-a -oxidase was investigated in comparison with other drugs. The results od this article, can be considered as one of a brand-new and effective methods for assessing the body sensitization caused by the use of enzymes and can contribute to the development of the respective field.

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Section: Methodsmentioning

confidence: 99%

Section: Fig 2: the Effect Of Immune Sera On The Activity Of L-lysine...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of L-Lysine-A-Oxidase, A New Antitumor And Antiviral Drug Substance Synthesized By Trichoderma

Smirnova

Кузнецов

Podoprigora

et al. 2022

Int J Life Sci Pharm Res

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“…Phenylalanine in serum was quantified by a fluorescence assay, 6–8 and threonine was measured by an absorption kinetics‐based method 9 . In another experiment, after single‐dose nimesulide (50 mg/kg), serum lysine was measured at 30 min after administering lysine (2 g/kg, PO) 10 . To measure urinary amino acid resorption, mice were housed in metabolic cages for urine collection after administration of nimesulide (50 mg/kg, PO).…”

Section: Methodsmentioning

confidence: 99%

“…9 In another experiment, after single-dose nimesulide (50 mg/kg), serum lysine was measured at 30 min after administering lysine (2 g/kg, PO). 10 To measure urinary amino acid resorption, mice were housed in metabolic cages for urine collection after administration of nimesulide (50 mg/kg, PO). The amount of phenylalanine, threonine and lysine in urine was measured using the methods described above for serum.…”

Section: Effect Of Nimesulide On Amino Acid Absorptionmentioning

confidence: 99%

In vivo antidiabetic activity of nimesulide due to inhibition of amino acid transport

Joharapurkar

Kshirsagar

Patel

et al. 2021

Basic Clin Pharma Tox

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Inhibiting the intestinal and renal neutral amino acid transporter B0AT1 by genetic means has improved insulin sensitivity in mice, but there are no antagonists available for preclinical or clinical use. Since the anti‐inflammatory agent nimesulide selectively inhibited B0AT1 in vitro, we hypothesized that nimesulide exhibits in vivo potential to restrict neutral amino acid absorption and, therefore, may improve insulin sensitivity. The dose‐related effect of nimesulide (10 to 100 mg/kg, PO) on intestinal absorption of neutral amino acids was estimated in C57 mice. The effect of nimesulide (50 mg/kg, PO) on renal resorption of amino acids was also assessed. The effect of chronic nimesulide (50 mg/kg, PO, BID for 14 days) was assessed in high protein diet‐fed C57 mice, diet‐induced obese mice and obese and diabetic db/db mice. Acute and chronic nimesulide treatment decreased absorption of neutral amino acids and increased their urinary excretion. Nimesulide treatment improved insulin sensitivity and glycemic control, increased GLP‐1, decreased liver lipids and improved FGF‐21 in serum. Nimesulide improved insulin sensitivity and glucose tolerance by inhibiting neutral amino acid transport in the intestine and kidneys. Thus, it can serve as a tool compound for in vivo B0AT1 inhibition.

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Cytotoxic activity of l-lysine alpha-oxidase against leukemia cells

Costa¹,

Silva²

2022

Seminars in Cancer Biology

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Plasma pharmacokinetics and tissue distribution of L-lysine α-oxidase from Trichoderma cf. aureoviride RIFAI VKM F- 4268D in mice

Cited by 6 publications

References 24 publications

Characterization of L-Lysine-A-Oxidase, A New Antitumor And Antiviral Drug Substance Synthesized By Trichoderma

Characterization of L-Lysine-A-Oxidase, A New Antitumor And Antiviral Drug Substance Synthesized By Trichoderma

In vivo antidiabetic activity of nimesulide due to inhibition of amino acid transport

Cytotoxic activity of l-lysine alpha-oxidase against leukemia cells

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