Plasma Peptide Concentrations and Peptide-Reactive Immunoglobulins in Patients with Eating Disorders at Inclusion in the French EDILS Cohort (Eating Disorders Inventory and Longitudinal Survey)

Abstract

Eating disorders (EDs) are increasingly frequent. Their pathophysiology involves disturbance of peptide signaling and the microbiota–gut–brain axis. This study analyzed peptides and corresponding immunoglobulin (Ig) concentrations in groups of ED. In 120 patients with restrictive (R), bulimic (B), and compulsive (C) ED, the plasma concentrations of leptin, glucagon-like peptide-1 (GLP-1), peptide YY (PYY), and insulin were analyzed by Milliplex and those of acyl ghrelin (AG), des-acyl ghrelin (DAG), and α-melanocyte-stimulating hormone (α-MSH) by ELISA kits. Immunoglobulin G (in response to an antigen) concentrations were analyzed by ELISA, and their affinity for the respective peptide was measured by surface plasmon resonance. The concentrations of leptin, insulin, GLP-1, and PYY were higher in C patients than in R patients. On the contrary, α-MSH, DAG, and AG concentrations were higher in R than in C patients. After adjustment for body mass index (BMI), differences among peptide concentrations were no longer different. No difference in the concentrations of the IgG was found, but the IgG concentrations were correlated with each other. Although differences of peptide concentrations exist among ED subtypes, they may be due to differences in BMI. Changes in the concentration and/or affinity of several anti-peptide IgG may contribute to the physiopathology of ED or may be related to fat mass.

Intestinal permeability and appetite regulating peptides-reactive immunoglobulins in severely malnourished women with anorexia nervosa

“…Previous studies suggested that aMSH-reactive Immunoglobulins were modified in patients with AN [32,33]. Our findings are in accordance with recent studies [24,25] showing that aMSH-reactive immunoglobulins are not modified in patients with AN compared with controls.…”

“…Affinity kinetics of plasma extracted IgG for acyl-ghrelin, desacyl-ghrelin, leptin and aMSH were analyzed by surface plasmon resonance (SPR) on a BIAcore T200 (GE Healthcare) according to previously published protocol [24]. Briefly, peptides were diluted in 10 mM sodium acetate buffer (pH 5.0) and coated on the CM5 sensor chip (GE Healthcare).…”

Current Aspects of the Role of Autoantibodies Directed Against Appetite-Regulating Hormones and the Gut Microbiome in Eating Disorders

“…High plasma levels and changes of affinity kinetics of autoantibodies reacting with a-MSH and ACTH seem to be caused by the exposure to stress as a result of concomitant hypothalamic-pituitary-adrenal (HPA) axis activation (110,111). These results support the hypothesis that changes in affinity of autoantibodies reacting with a-MSH and ACTH are involved in the pathogenesis of AN and BN and that increased levels of high-affinity anti-a-MSH or anti-ClpB (a-MSH conformational mimetic produced by Enterobacteriaceae) autoantibodies can induce bulimia, while increased levels of low-affinity anti-a-MSH autoantibodies can induce anorexia (24,110,112) (Table 1).…”

Cerebrospinal fluid cholinergic biomarkers are associated with postoperative delirium in elderly patients undergoing Total hip/knee replacement: a prospective cohort study

“…We reversed the anticoagulant tube back and forth 10-12 times so that the blood and anticoagulant could mix well. And the blood samples were centrifuged immediately at 2000 g for 10 min at room temperature for the collection of plasma [15], which were stored at − 80°C until further analysis.…”