Plasma nitrotyrosine levels, antioxidant vitamins and hyperglycaemia

Bo, Simona; Gambino, Roberto; Guidi, Sabrina; Silli, Barbara; Gentile, Luigi; Cassader, Maurizio; Pagano, Gianfranco

doi:10.1111/j.1464-5491.2005.01588.x

Cited by 20 publications

(12 citation statements)

References 27 publications

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“…NO 2 Tyr concentration and ratio to Tyr were elevated in smoker's plasma, being 3.8 and 3.9 times higher, respectively, than that of non-smoker controls. No age-dependence of NO 2 Tyr was found in this study (data not shown, p > 0.05), in agreement with the published data (Soderling et al, 2003;Bo et al, 2005). Plasma NO 2 Tyr was normally distributed with no sex differences in the healthy population studied (p > 0.05).…”

Section: Plasma Concentrations In Smokers and Healthy Human Volunteerssupporting

confidence: 94%

Determination of plasma free 3‐nitrotyrosine and tyrosine by reversed‐phase liquid chromatography with 4‐fluoro‐7‐nitrobenzofurazan derivatization

Zhang

Lang

Kaye

2007

Biomedical Chromatography

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Oxidative stress plays an important role in pathogenesis of many diseases. Measurement of 3-nitrotyrosine (NO(2)Tyr), as a potential biomarker for nitric oxide-mediated damage, has recently been the focus of particular attention. We have developed an HPLC method with NBD-F pre-column derivatization followed by C(18) cartridge cleaning. Using this method we achieved limits of detection of 0.5 and 1.1 nm for NO(2)Tyr and tyrosine (Tyr), respectively, close to that achieved by LS-MS/MS. NO(2)Tyr and tyrosine concentrations were linear over the calibration ranges 0.5-100 nm and 1-320 microm, respectively, with correlation coefficients greater than 0.95. To evaluate the utility of this assay in plasma we analysed samples obtained from smokers and non-smoking subjects. Consistent with the presence of elevated oxidative stress, the plasma NO(2)Tyr concentration and NO(2)Tyr:Tyr ratio of smokers were 17.42 +/- 11.6 nm and 0.263 +/- 0.192 nm/microm with 3.8 and 3.9 times higher (both p < 0.05), respectively, than that of non-smoker controls (4.54 +/- 2.75 nm and 0.067 +/- 0.050 nm/microm, respectively). In conclusion, we have developed a novel HPLC assay for NO(2)Tyr without MS detection that is applicable to clinical studies addressing the pathophysiology and importance of oxidative stress.

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Section: Plasma Concentrations In Smokers and Healthy Human Volunteerssupporting

confidence: 94%

Determination of plasma free 3‐nitrotyrosine and tyrosine by reversed‐phase liquid chromatography with 4‐fluoro‐7‐nitrobenzofurazan derivatization

Zhang

Lang

Kaye

2007

Biomedical Chromatography

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“…Accumulation of this group of modifications is considered a marker of One of the most studied irreversible modification is tyrosine nitration, the result of peroxynitrite reaction with the OH moiety in the tyrosine side chain (198,342,434). Along the discussion in section IC, increased total serum protein carbonylation and nitrotyrosine levels have been reported in diabetes (37,50,426), potentially implicating both ROS, RNS, and active carbonyls (like 4-HNE) in systemic protein modification associated with this disorder. In cells, altered protein function occurred in response to in vitro oxidation/nitration.…”

Section: Direct Modification Of Insulin Signalingmentioning

confidence: 99%

Positive and Negative Regulation of Insulin Signaling by Reactive Oxygen and Nitrogen Species

Bashan¹,

Kovsan²,

Kachko³

et al. 2009

Physiological Reviews

468

367

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Regulated production of reactive oxygen species (ROS)/reactive nitrogen species (RNS) adequately balanced by antioxidant systems is a prerequisite for the participation of these active substances in physiological processes, including insulin action. Yet, increasing evidence implicates ROS and RNS as negative regulators of insulin signaling, rendering them putative mediators in the development of insulin resistance, a common endocrine abnormality that accompanies obesity and is a risk factor of type 2 diabetes. This review deals with this dual, seemingly contradictory, function of ROS and RNS in regulating insulin action: the major processes for ROS and RNS generation and detoxification are presented, and a critical review of the evidence that they participate in the positive and negative regulation of insulin action is provided. The cellular and molecular mechanisms by which ROS and RNS are thought to participate in normal insulin action and in the induction of insulin resistance are then described. Finally, we explore the potential usefulness and the challenges in modulating the oxidant-antioxidant balance as a potentially promising, but currently disappointing, means of improving insulin action in insulin resistance-associated conditions, leading causes of human morbidity and mortality of our era.

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“…All participants completed a FFQ and underwent several clinical and laboratory tests (11). Three hundred men from this populationbased cohort were randomly identified (12); their TAS and NT concentrations were measured.…”

Section: Methodsmentioning

confidence: 99%

Associations of Dietary and Serum Copper with Inflammation, Oxidative Stress, and Metabolic Variables in Adults ,

Durazzo

Gambino

et al. 2008

The Journal of Nutrition

142

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There are conflicting data on the associations between copper and glycemia, plasma lipids, and atherosclerotic diseases.Copper has both pro-oxidant and antioxidant effects. We performed a cross-sectional analysis to investigate the associations between dietary copper intake and metabolic variables and serum high-sensitivity C-reactive protein (hs-CRP) in asymptomatic subjects from a population-based cohort (n ¼ 1197) and between serum copper concentration and markers of oxidative stress, including plasma nitrotyrosine (NT) and total antioxidant status (TAS), hs-CRP, and metabolic variables in a subgroup of men from this cohort (n ¼ 231). In all subjects, diastolic blood pressure and circulating glucose, uric acid, and total and LDL-cholesterol concentrations significantly decreased, whereas the hs-CRP concentration increased, from the lowest to the highest tertile of copper intake. In the male subgroup, glucose and total and LDL-cholesterol and TAS decreased, whereas hs-CRP and NT concentrations increased from the lowest to the highest tertile of serum copper concentration. In multiple regression models, dietary copper intake was inversely associated with diastolic blood pressure (P ¼ 0.002), fasting glucose (P , 0.001), total cholesterol (P , 0.001), LDL-cholesterol (P , 0.001), and uric acid (P , 0.001) and was directly associated with the hs-CRP concentration (P , 0.001). Serum copper concentrations were inversely associated with glucose (P , 0.001), total cholesterol (P , 0.001), LDL-cholesterol (P , 0.001), and TAS (P , 0.001) and were directly associated with hs-CRP (P , 0.001) and NT concentrations (P , 0.001). Marginal copper deficiency is associated with an unfavorable metabolic pattern, but copper supplementation might not be recommended in view of its association with inflammation and markers of oxidative stress.

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Plasma nitrotyrosine levels, antioxidant vitamins and hyperglycaemia

Cited by 20 publications

References 27 publications

Determination of plasma free 3‐nitrotyrosine and tyrosine by reversed‐phase liquid chromatography with 4‐fluoro‐7‐nitrobenzofurazan derivatization

Determination of plasma free 3‐nitrotyrosine and tyrosine by reversed‐phase liquid chromatography with 4‐fluoro‐7‐nitrobenzofurazan derivatization

Positive and Negative Regulation of Insulin Signaling by Reactive Oxygen and Nitrogen Species

Associations of Dietary and Serum Copper with Inflammation, Oxidative Stress, and Metabolic Variables in Adults ,

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