Plasma nitric oxide (nitrate/nitrite) levels in acute stroke and their relationship with severity and outcome

Rashid, Parveen A.; Whitehurst, A.; Lawson, Nigel; Bath, Philip M.W.

doi:10.1053/jscd.2003.9

Cited by 78 publications

(70 citation statements)

References 25 publications

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“…However, GTN forms NO, which is a potent modulator of cerebrovascular reactivity, especially in collateral vessels, such as pial arteries. 25,26 Vascular NO levels are low in stroke, 27 so collateral vessels may not be maximally dilated. Hence, CBF might be held constant with GTN if moderate reductions in systemic BP were counterbalanced by increases in collateral blood supply, which would be potentially beneficial in acute stroke.…”

Section: Discussionmentioning

confidence: 99%

Transdermal Glyceryl Trinitrate Lowers Blood Pressure and Maintains Cerebral Blood Flow in Recent Stroke

et al. 2006

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Abstract-High blood pressure (BP) is common in acute stroke and is independently associated with a poor outcome.Lowering BP might improve outcome if it did not adversely affect cerebral blood flow (CBF) or cerebral perfusion pressure. We investigated the effect of glyceryl trinitrate ([GTN] an NO donor) on quantitative CBF, BP, and cerebral perfusion pressure in patients with recent stroke. Eighteen patients with recent (Ͻ5 days) ischemic (nϭ16) or hemorrhagic (nϭ2) stroke were randomly assigned (2:1) to transdermal GTN (5 mg) or control. CBF (global, hemispheric, arterial territory, and lesion, using xenon computed tomography) and BP (peripheral and central) were measured before and 1 hour after treatment with GTN. The effects of GTN on CBF and BP were adjusted for baseline measurements (ANCOVA

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Section: Discussionmentioning

confidence: 99%

Transdermal Glyceryl Trinitrate Lowers Blood Pressure and Maintains Cerebral Blood Flow in Recent Stroke

et al. 2006

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“…38,39 Similarly, acute stroke is associated with low NOx and high vWf levels. 18,40 Although our trials were small, we used a crossover design (thereby allowing each subject to act as their own control) which considerably increases statistical power as compared with a parallel group design. Additionally, we assessed the effects of antiplatelet agents in two different sets of subjects which increases the validity of the findings.…”

Section: Discussionmentioning

confidence: 99%

“…17 This involved injecting 10µL of deproteinised plasma samples into vanadium chloride. 18 The nitrate and nitrite present in samples were reduced to NO, and quantified from a calibration curve based on prepared concentrations of sodium nitrate.…”

Section: Laboratory Studymentioning

confidence: 99%

Effect of aspirin, clopidogrel and dipyridamole on soluble markers of vascular function in normal volunteers and patients with prior ischaemic stroke

et al. 2006

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A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk IL-6 also increases endothelial production of the fibrinolytic inhibitor, plasminogen activator inhibitor type 1 (PAI-1). We assessed the effects of aspirin, clopidogrel and dipyridamole on a panel of the above soluble markers of vascular function in normal volunteers and patients with a prior history of ischaemic stroke. We also assessed the effect of combining the three antiplatelet agents on the soluble markers since we have shown previously that three drugs may be superior to one or two in inhibiting platelet function in vitro. 15Page 4 of 15 METHODS DesignTwo randomised, outcome-blinded, multiway, crossover trials of antiplatelet therapy were performed in normal volunteers and patients with prior ischaemic stroke. The study design has been published previously 16 in a report of the effect of aspirin, clopidogrel and dipyridamole on the function of circulating platelets, phagocytes and their conjugates. SubjectsThe study protocol was approved by the local Research Ethics Committee. All subjects gave written informed consent and the trials were performed according to the that complete data were present for each person; laboratory data from withdrawing subjects are not included in the statistical analyses. Laboratory studySerum and plasma collected into 3.8% sodium citrate (1:9 volume) or 4.0 mmol/L EDTA were centrifuged (1,500g for 15min) within 1 hour of venepuncture. Aliquots were frozen at -80 o C and then assayed in batches using commercially available ELISA kits. CRP (high sensitivity kit, Kalon Biological, Hants, UK) and PDGF (R&D System, Abington, UK) were measured in serum; cGMP (R&D System), vWf (Corgenix, Cambridgeshire, UK), MCP-1 (R&D System) and PAI-1 (Technoclone TC, Surrey, UK)were analysed in citrated plasma. Statistical analysisThe primary analysis compared the presence and absence of each drug on soluble markers, i.e. aspirin versus no aspirin, clopidogrel versus no clopidogrel, and dipyridamole versus no dipyridamole. Secondary analyses compared triple therapy (ACD) versus all other drugs, as before. 16 Analyses were performed using the SAS statistical package.Page 6 of 15 RESULTSDemographic and clinical information for the subjects are given in table 1. The stroke patients are representative of our clinical stroke service and were significantly older and had more risk factors (more hypertension and smokers, higher systolic blood pressure, and elevated total cholesterol) than the volunteers (all p<0.05).When considering the individual effects of the three antiplatelet agents on soluble markers of vascular function, three observations were made. First, dipyridamole significantly reduced plasma vWf levels in ...

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“…In this study, large artery ischemic stroke have been showed profound lower NO and an increasing H 2 O 2 levels which they are similar to small artery stroke that we have reported recently. It has been recently reported that nitrite, a major metabolite of NO is high persistently in stroke [40]. Lower NO during stroke may be resulted from peroxynitrite formation which it is derived from NO and superoxide reaction.…”

Section: Discussionmentioning

confidence: 99%

Neurovascular Oxidative Stress and Autonomic Modulation Contributing to QT Interval Variations in Acute Large Artery Ischemic Stroke

Suwanprasert¹

2017

Austin J Cerebrovasc Dis & Stroke

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Plasma nitric oxide (nitrate/nitrite) levels in acute stroke and their relationship with severity and outcome

Cited by 78 publications

References 25 publications

Transdermal Glyceryl Trinitrate Lowers Blood Pressure and Maintains Cerebral Blood Flow in Recent Stroke

Transdermal Glyceryl Trinitrate Lowers Blood Pressure and Maintains Cerebral Blood Flow in Recent Stroke

Effect of aspirin, clopidogrel and dipyridamole on soluble markers of vascular function in normal volunteers and patients with prior ischaemic stroke

Neurovascular Oxidative Stress and Autonomic Modulation Contributing to QT Interval Variations in Acute Large Artery Ischemic Stroke

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