Plasma nitrate+nitrite levels are regulated by ovarian steroids but do not correlate with trabecular bone mineral density in rats

Bezooijen, Rutger L. van; Que, Ivo; Ederveen, A. G. H.; Kloosterboer, Helenius J.; Papapoulos, Socrates E.; Löwik, Clemens W.G.M.

doi:10.1677/joe.0.1590027

Cited by 52 publications

(34 citation statements)

References 48 publications

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“…Reduced cofactor availability for eNOS can decrease NO production 19. Previous reports show that OVX significantly lowers plasma NO metabolite [NO x; plasma nitrate+nitrite] levels in rats 55. In postmenopausal women receiving hormone replacement therapy (HRT), plasma NO x levels are positively correlated with estrogen status 56.…”

Section: Discussionmentioning

confidence: 99%

Increasing dietary nitrate has no effect on cancellous bone loss or fecal microbiome in ovariectomized rats

Conley

Roberts

Sharpton

et al. 2017

Molecular Nutrition Food Res

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ScopeStudies suggest diets rich in fruit and vegetables reduce bone loss, although the specific compounds responsible are unknown. Substrates for endogenous nitric oxide (NO) production, including organic nitrates and dietary nitrate, may support NO production in age‐related conditions, including osteoporosis. We investigated the capability of dietary nitrate to improve NO bioavailability, reduce bone turnover and loss.Methods and resultsSix‐month‐old Sprague Dawley rats [30 ovariectomized (OVX) and 10 sham‐operated (sham)] were randomized into three groups: (i) vehicle (water) control, (ii) low‐dose nitrate (LDN, 0.1 mmol nitrate/kg bw/day), or (iii) high‐dose nitrate (HDN, 1.0 mmol nitrate/kg bw/day) for three weeks. The sham received vehicle. Serum bone turnover markers; bone mass, mineral density, and quality; histomorphometric parameters; and fecal microbiome were examined. Three weeks of LDN or HDN improved NO bioavailability in a dose‐dependent manner. OVX resulted in cancellous bone loss, increased bone turnover, and fecal microbiome changes. OVX increased relative abundances of Firmicutes and decreased Bacteroideceae and Alcaligenaceae. Nitrate did not affect the skeleton or fecal microbiome.ConclusionThese data indicate that OVX affects the fecal microbiome and that the gut microbiome is associated with bone mass. Three weeks of nitrate supplementation does not slow bone loss or alter the fecal microbiome in OVX.

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Section: Discussionmentioning

confidence: 99%

Increasing dietary nitrate has no effect on cancellous bone loss or fecal microbiome in ovariectomized rats

Conley

Roberts

Sharpton

et al. 2017

Molecular Nutrition Food Res

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“…Quantitative sandwich enzyme immunoassay technique used for determination of sE-cadherin and sAPO-1/Fas by commercial kits (USA). Serum level of NO measured by colorimetric method as total nitrite concentration [13]. Cotinine measured by chemical method according to Peach et al [14].…”

Section: Biochemical Assaysmentioning

confidence: 99%

Clinical and Biochemical Effects of Environmental Tobacco Smoking On Pregnancy Outcome

et al. 2012

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The effects of environmental tobacco smoke (ETS) are less studied especially on neonates. This study evaluates the clinical and biochemical effects in neonates exposed to ETS during pregnancy. Two hundred pregnant women asked to complete the questioners about their ETS. Ninety from them were enrolled in biochemical assays as two groups according to ETS. The cotinine level determined in saliva and serum of mothers to confirm their tobacco exposure. The routine tracheal suction from the fetus was used to determine the level of neuron specific enolase (NSE), soluble E-cadherin, sApo-1/Fas, nitric oxide (NO) and cotinine. In clinical assessment, the percent of full term babies in nonexposed group (72 %) are higher compared to exposed group (67 %). Apgar score at the first min, admission to intensive care unit (ICU) and morbidity during the first month shows statistical significance increase in exposed compared to non-exposed group (p = 0.03, 0.05, 0.01, respectively). The new born weight in exposed group significantly decreased compared to non-exposed group (2,850 g ± 3.74 vs 2,967.67 g ± 3.34; p = 0.02). In biochemical assessment, NSE and sE-cadherin significantly increased, while NO significantly decreased (p = 0.000) in exposed compared to non-exposed group. There is a positive correlation between level of cotinine and both NSE, sE-cadherin (r = 0.7, 0.9; p = 0.000, 0.006, respectively). To our knowledge, this is the first study link between prenatal tobacco exposure (PTE) and biochemical parameters measured in tracheal suction. PTE will lead to decrease in birth weight most probably by decreasing NO, sFas, and increasing sE-cadherin. While, increased morbidity of neonates in the exposed group could be attributed to cessation of breast feeding and its complication and increased NSE in the studied markers.

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“…Plasma level ACE activities were determined by using furylacrylyl phenylalanyl glycylglycine (FAPGG) as substrate as described previously. 19 NO levels were measured as total nitrite concentration after reduction of nitrate to nitrite according to the method described by van Bezooijen et al 20 Plasma cGMP levels were measured using an immunoradiometric assay kit supplied by IBL GmbH (Cat. #29071/75, Flughafenstrasse, Hamburg, Germany) according to the manufacturer's protocol.…”

Section: Biochemical Analysismentioning

confidence: 99%

Role of some vasoactive mediators in patients with erectile dysfunction: their relationship with angiotensin-converting enzyme and growth hormone

et al. 2003

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The imbalance between vasoconstrictors and vasodilators may play an important role in the pathogenesis of erectile dysfunction (ED). A total of 36 patients with ED, organogenic [diabetic (n ¼ 12) and nondiabetic (n ¼ 12)] and psychogenic (n ¼ 12) etiology, and 12 healthy adult men as controls were included. The levels of endothelin-1 (ET-1), growth hormone (GH), angiotensinconverting enzyme activity (ACE), nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) were determined in the flaccid penis cavernosal blood of patients and in cubital blood of patients and controls. In psychogenic ED, systemic ACE activity was elevated compared to controls (Po0.05). In diabetic and nondiabetic ED patients, systemic levels of ET-1 (Po0.0001 for both) and ACE activity (Po0.01 and o0.05) were higher while GH (Po0.0001 and o0.001), NO (Po0.0001 for both) and cGMP (Po0.01 for both) levels were lower compared to controls. In diabetic patients, systemic and cavernosal ET-1 levels (Po0.0001 for both) and cavernosal ACE activity levels (Po0.05) were significantly elevated while systemic and cavernosal NO (Po0.0001 for both) and GH (o0.001 and o0.05) levels were declined compared to psychogenic. In nondiabetic patients, systemic and cavernosal ET-1 levels (Po0.0001 for both) were significantly elevated while systemic and cavernosal NO (Po0.0001 for both) and systemic GH levels (Po0.05) were declined compared to psychogenic. Systemic NO was positively correlated with GH in psychogenic (r ¼ 0.616, Po0.05), diabetic (r ¼ 0.583, Po0.05) and nondiabetic (r ¼ 0.615, Po0.05) patients and correlated positively with cGMP (r ¼ 0.605, Po0.05) but negatively with ACE activities (r ¼ À0.585, Po0.05) in diabetic patients. In conclusion, plasma levels of ET-1, ACE activities are elevated and associated with reduction of GH, NO and cGMP levels in the systemic and cavernous blood of ED patients. This disturbance may indicate endothelial dysfunction that may hind at their significance in the pathophysiology of ED.

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Plasma nitrate+nitrite levels are regulated by ovarian steroids but do not correlate with trabecular bone mineral density in rats

Cited by 52 publications

References 48 publications

Increasing dietary nitrate has no effect on cancellous bone loss or fecal microbiome in ovariectomized rats

Increasing dietary nitrate has no effect on cancellous bone loss or fecal microbiome in ovariectomized rats

Clinical and Biochemical Effects of Environmental Tobacco Smoking On Pregnancy Outcome

Role of some vasoactive mediators in patients with erectile dysfunction: their relationship with angiotensin-converting enzyme and growth hormone

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