Plasma Myoglobin in the Early Diagnosis of Acute Myocardial Infarction

Ercolini, P; Forino, Francesca; Basevi, A; Vrenna, L.; Castaldo, Paola; D'Ambrosio, V.; Castaldo, Anna

doi:10.1515/cclm.1994.32.5.349

Cited by 16 publications

(12 citation statements)

References 14 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An overview of the 30 relevant articles on myoglobin is outlined in table 3 15 18 20 24 28 32–44 48–59. All studies but one were performed in the correct study domain and all studies had the relevant outcome AMI (WHO n=23, Joint ESC/ACC consensus 2000 n=5) or ACS (n=2).…”

Section: Resultsmentioning

confidence: 99%

Novel biochemical markers in suspected acute coronary syndrome: systematic review and critical appraisal

Dekker

Mosterd

Hof

et al. 2010

Heart

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Novel biochemical markers in suspected acute coronary syndrome: systematic review and critical appraisal

Dekker

Mosterd

Hof

et al. 2010

Heart

View full text Add to dashboard Cite

show abstract

“…Thus, this new concept of electropolymerization for imprinting a protein cardiac biomarker is here described, using Myo as target protein. Myo is a heme protein responsible for the transport of oxygen within cardiac and skeletal muscle cells [35,36] that is released early in the blood in the course of cardiac muscle damage. The measurement of Myo levels within the first few hours after symptom onset in acute events is crucial to diagnosis of cardiac injury.…”

Section: Introductionmentioning

confidence: 99%

“…The measurement of Myo levels within the first few hours after symptom onset in acute events is crucial to diagnosis of cardiac injury. Typical concentrations range from 95 to 472 µg/L [35,[37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Protein-responsive polymers for point-of-care detection of cardiac biomarker

Moreira

Sharma

Dutra

et al. 2014

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made respon-sive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing prot ein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications.Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the mate-rial to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The d evices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 µg/mL, respectively, with detection limits of 1.5 and 0.8 µg/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivit y and selectivity.

show abstract

“…Although Myo levels should not be used as the sole biomarker for the diagnosis of ACS, a normal Myo concentration within 2 h after presentation may exclude AMI [4,6]. Myo cut-off levels range from 100 to 200 ng·mL −1 [7,8], reaching levels of 420-2000 ng· mL −1 in serum [9] and 450 ng· mL −1 in the urine [10]. Screening Myo in an emergency context is therefore important, and a suitable point-of-care (POC) assay would result in a reduced time to diagnosis and treatment [11].…”

Section: Introductionmentioning

confidence: 99%

Detection of cardiac biomarker proteins using a disposable based on a molecularly imprinted polymer grafted onto graphite

Moreira

Sharma

Dutra³

et al. 2014

Microchim Acta

View full text Add to dashboard Cite

A low-cost disposable was developed for rapid detection of the protein biomarker myoglobin (Myo) as a model analyte. A screen printed electrode was modified with a molecularly imprinted material grafted on a graphite support and incorporated in a matrix composed of poly(vinyl chloride) and the plasticizer o-nitrophenyloctyl ether. The proteinimprinted material (PIM) was produced by growing a reticulated polymer around a protein template. This is followed by radical polymerization of 4-styrenesulfonic acid, 2-aminoethyl methacrylate hydrochloride, and ethylene glycol dimethacrylate. The polymeric layer was then covalently bound to the graphitic support, and Myo was added during the imprinting stage to act as a template. Non-imprinted control materials (CM) were also prepared by omitting the Myo template. Morphological and structural analysis of PIM and CM by FTIR, Raman, and SEM/EDC microscopies confirmed the modification of the graphite support. The analytical performance of the SPE was assessed by square wave voltammetry. The average limit of detection is 0.79 μg of Myo per mL, and the slope is −0.193±0.006 μA per decade. The SPE-CM cannot detect such low levels of Myo but gives a linear response at above 7.2 μg·mL −1 , with a slope of −0.719± 0.02 μA per decade. Interference studies with hemoglobin, bovine serum albumin, creatinine, and sodium chloride demonstrated good selectivity for Myo. The method was successfully applied to the determination of Myo urine and is conceived to be a promising tool for screening Myo in point-ofcare patients with ischemia.

show abstract

Plasma Myoglobin in the Early Diagnosis of Acute Myocardial Infarction

Cited by 16 publications

References 14 publications

Novel biochemical markers in suspected acute coronary syndrome: systematic review and critical appraisal

Novel biochemical markers in suspected acute coronary syndrome: systematic review and critical appraisal

Protein-responsive polymers for point-of-care detection of cardiac biomarker

Detection of cardiac biomarker proteins using a disposable based on a molecularly imprinted polymer grafted onto graphite

Contact Info

Product

Resources

About