Plasma miR-940 may serve as a novel biomarker for gastric cancer

Liu, Xin; Kwong, Ava; Sihoe, Alan D. L.; Chu, Kent‐Man

doi:10.1007/s13277-015-4019-5

Cited by 37 publications

(31 citation statements)

References 42 publications

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“…The expression levels of miRNAs vary by cell type and condition. Thus, in referring to the literature, we found that the overexpressions of miR-940 in pancreatic carcinoma (26) and gastric cancer (27) were correlated with tumor progression. However, the role of miR-940 in ovarian cancer is unknown.…”

Section: Hypoxia Induces Overexpression Of Mir-940 Both In Eoc Cells mentioning

confidence: 68%

Exosomes derived from hypoxic epithelial ovarian cancer deliver microRNA-940 to induce macrophage M2 polarization

et al. 2017

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Hypoxia is a common feature of solid tumors. It is closely related to tumor progression. Exosomal microRNAs derived from cancers are considered to be mediators between cancer cells and the tumor microenvironment. In addition, the number of tumor-associated macrophages (TAMs) in the tumor microenvironment has also been demonstrated to correlate with tumor development. However, the relationship between tumor-secreted exosomes and TAM polarization under hypoxic conditions during tumor progression is not clear. Herein, we demonstrated that hypoxia induces the high expression of microRNA-940 (miR‑940) in exosomes derived from epithelial ovarian cancer (EOC). We also found that miR‑940 is highly expressed in exosomes isolated from ascites of EOC patients. Moreover, the overexpression of miR‑940 in macrophages delivered by exosomes stimulated M2 phenotype polarization, while the M2 subtype macrophages promoted EOC proliferation and migration. These results highlight the function of hypoxia in enhancing the high level of expression of miR‑940 in tumor exosomes taken up by macrophages. We also showed that the tumor-promoting function of miR‑940 is mediated by TAM polarization in EOC. These findings show that tumor-derived exosomal miR‑940 induced by hypoxia plays an important role in stimulating TAM polarization in the progression of EOC.

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Section: Hypoxia Induces Overexpression Of Mir-940 Both In Eoc Cells mentioning

confidence: 68%

Exosomes derived from hypoxic epithelial ovarian cancer deliver microRNA-940 to induce macrophage M2 polarization

et al. 2017

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“…Ectopic expression of miRNA-133a inhibited GC cell proliferation, migration, and invasion, and induced cell apoptosis [33]. Decreased plasma miRNA-940 may serve as a novel diagnostic biomarker for GC [34]. Overexpression of miRNA-23b-3p reversed GC cell resistance to multiple chemotherapeutics in vitro [35].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic and Prognostic Value of Serum MicroRNA-206 in Patients with Gastric Cancer

Hou

Luo

2016

Cell Physiol Biochem

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Aims: Recent studies have demonstrated that microRNAs (miRNAs) can serve as useful biomarkers for human cancers. The aim of this study was to evaluate the expression level of serum miRNA-206 in patients with gastric cancer (GC) and investigate its diagnostic and prognostic value. Methods: Quantitative real-time PCR was performed to evaluate serum miRNA-206 levels in 150 GC patients and 150 healthy volunteers. The association between miRNA-206 expression and clinicopathological factors as well as patient's survival was analyzed. Receiver operator curve (ROC) analysis was carried out to assess the potential value of serum miRNA-206 for GC diagnosis. Results: Serum miRNA-206 was down-regulated in GC patients compared with healthy controls (P < 0.001). Decreased serum miRNA-206 expression was significantly associated with deep local invasion, positive lymph node metastasis, and advanced clinical stage. Serum miRNA-206 expression was found to be significantly up-regulated in paired post-operative samples and reduced in patients with GC recurrence. ROC curve analysis showed that serum miRNA-206 was a useful marker for GC diagnosis, and could discriminate between recurred and non-recurred patients. Multivariate Cox regression analysis confirmed low serum miRNA-206 expression as an independent unfavorable prognostic factor for both DFS and OS of GC patients. Conclusions: These results suggest that serum miRNA-206 might not only serve as a novel diagnostic biomarker for GC, but also predict cancer recurrence and patient's prognosis.

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“…Patients with hepatocellular carcinoma and low miR-940 expression levels exhibited poorer prognosis compared with high expression levels (18). miR-940 was additionally demonstrated to be downregulated in prostate (20), breast (21) and ovarian cancer (22); however, miR-940 expression was observed to be upregulated in gastric cancer tissues, cell lines and plasma (25). High miR-940 expression levels were strongly correlated with the advanced N stage of gastric cancer (19).…”

Section: Discussionmentioning

confidence: 94%

“…MicroRNAs (miRNAs) comprise a series of endogenous, short single-stranded non-coding RNAs (19)(20)(21)(22)(23)(24)(25) molecules) that primarily serve as gene regulators (8). These molecules negatively regulate gene expression by directly binding to partial complimentary sites in the 3'-untranslated regions (3'-UTRs) of their target genes, resulting in mRNA degradation or inhibition of translation (9).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑940 promotes cell proliferation and invasion of glioma by directly targeting Kruppel‑like factor�9

et al. 2018

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has been extensively studied in the pathogenesis of numerous types of human cancer; however, the expression pattern, roles and molecular mechanisms underlying the regulatory actions of miR-940 in glioma remain unknown. The present study aimed to further investigate miR-940 by studying its expression, roles and mechanisms of action in glioma. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect miR-940 expression in glioma tissues and cell lines. The regulatory effects of miR-940 in glioma cell proliferation and invasion were determined using MTT and cell invasion assays. Bioinformatics analyses was performed to identify the potential target of miR-940, which was further confirmed by luciferase reporter assay, RT-qPCR and western blot analysis. In the present study, significantly increased miR-940 expression levels were observed in glioma tissues and cell lines compared with normal brain tissues and normal human astrocytes, respectively. Decreased miR-940 expression levels attenuated glioma cell proliferation and invasion in vitro. Kruppel-like factor 9 (KLF9) was predicted as a potential target of miR-940. Further assays demonstrated that miR-940 negatively regulated KLF9 expression in glioma cells by directly targeting the 3'-untranslated regions of KLF9. Additionally, KLF9 expression was downregulated in glioma tissues and was inversely correlated with miR-940. Furthermore, KLF9 knockdown was able to rescue the effects of miR-940 on glioma cell proliferation and invasion. The results of the present study suggest that miR-940 may function as an oncogene in glioma by targeting KLF9 and may be a considered a therapeutic target for the treatment of gliomas.

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Plasma miR-940 may serve as a novel biomarker for gastric cancer

Cited by 37 publications

References 42 publications

Exosomes derived from hypoxic epithelial ovarian cancer deliver microRNA-940 to induce macrophage M2 polarization

Exosomes derived from hypoxic epithelial ovarian cancer deliver microRNA-940 to induce macrophage M2 polarization

Diagnostic and Prognostic Value of Serum MicroRNA-206 in Patients with Gastric Cancer

MicroRNA‑940 promotes cell proliferation and invasion of glioma by directly targeting Kruppel‑like factor�9

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