Plasma miR‐122 and miR‐200 family are prognostic markers in colorectal cancer

Maierthaler, Melanie; Benner, Axel; Hoffmeister, Michael; Surowy, Harald; Jansen, Lina; Knebel, Phillip; Chang‐Claude, Jenny; Brenner, Hermann; Burwinkel, Barbara

doi:10.1002/ijc.30433

Cited by 100 publications

(80 citation statements)

References 52 publications

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“…Moreover, miR‐122‐5p was potential regulator of several BC‐correlated targets such as ADAM10, Syndecan‐1 and IGF1R . The diagnostic or prognostic value of circulating miR‐122 was also reported in other cancers such as colorectal cancer and non‐small cell lung cancer, indicating its close connection with tumor biological processes . Circulating miR‐215 was previously found to be decreased in plasma in progressive BC patients in comparison with those who did not, but van Schooneveld et al showed just the opposite result of increasing tendency in serum in metastatic BC patients .…”

Section: Discussionmentioning

confidence: 99%

A five‐miRNA panel in plasma was identified for breast cancer diagnosis

Zou

Xia

et al. 2019

Cancer Medicine

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Breast cancer (BC) is one of the most common cancers in females. Since early detection can bring prognosis benefit, discovery of novel noninvasive biomarkers for BC diagnosis is in urgent need. In this four‐phase study, we profiled miRNA expression in plasma samples from a total of 257 BC patients and 257 normal controls (NCs). Exiqon miRNA qPCR panel was used to select candidate miRNAs in the screening phase which were further analyzed using qRT‐PCR in the following training, testing and external validation phases. Finally, we identified five plasma miRNAs (let‐7b‐5p, miR‐122‐5p, miR‐146b‐5p, miR‐210‐3p and miR‐215‐5p) whose expression levels were significantly different between BC patients and NCs. A 5‐miRNA panel in plasma with high sensitivity and specificity was then constructed to detect BC. The areas under the receiver‐operating characteristic curves (AUCs) of the panel were 0.683, 0.966, 0.978 for the training, testing and external validation sets, respectively. Expression of the identified miRNAs was further analyzed among 32 pairs of BC tissue and the adjacent normal tissue samples as well as plasma‐derived exosome samples from 32 BC patients vs 32 NCs. Let‐7b‐5p was contrarily down‐regulated in BC tissue. In exosomes samples, only miR‐122‐5p was significantly up‐regulated as in plasma for BC patients. In conclusion, we identified a 5‐miRNA plasma panel (let‐7b‐5p, miR‐122‐5p, miR‐146b‐5p, miR‐210‐3p and miR‐215‐5p) that could serve as a promising biomarker for BC detection.

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Section: Discussionmentioning

confidence: 99%

A five‐miRNA panel in plasma was identified for breast cancer diagnosis

Zou

Xia

et al. 2019

Cancer Medicine

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“…A number of different microRNAs have been shown to be differentially expressed in patients with CRC versus healthy controls. 45,53 Variability in the timing of serum sampling may be an important factor. 55 miR-200c was individually shown to have good predictive values in the diagnosis of CRC.…”

Section: Discussionmentioning

confidence: 99%

“…Some of this variation may be accounted for by, as the authors comment, the fact that the some of the blood samples were hemolyzed and that phlebotomy was performed at variable time-points in relation to the date of surgery and to adjuvant therapy 53. High plasma expression of miR-200a was associated with worse OS in patients with metastatic CRC (HR 5 1.227, 95% CI: 1.008-1.495, p 5 0.042).…”

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confidence: 99%

The role of the miR‐200 family in epithelial–mesenchymal transition in colorectal cancer: a systematic review

O’Brien

Carter

Burton

et al. 2018

Intl Journal of Cancer

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Colorectal cancer (CRC) is associated with significant morbidity and mortality as many patients are diagnosed with advanced stage disease. MicroRNAs are small, noncoding RNA molecules that have a major role in gene expression regulation and are dysregulated in CRC. The miR-200 family is involved in epithelial-mesenchymal transition (EMT). This systematic review describes the roles of the miR-200 family in EMT in CRC. A search of electronic databases (PubMed and Embase) was conducted between January 2000 and July 2017. Both in vitro and human studies reporting on the miR-200 family and CRC were included. Studies describing molecular pathways and the role of the miR-200 family in the diagnostic and therapeutic management of CRC were analyzed. Thirty-four studies (22 in vitro and 18 human studies) were included. miR-200 family expression is regulated epigenetically and via transcriptional factor regulation. In vitro studies show that transfection of miR-200 family members into chemo-resistant colon cancer cell lines results in improved chemo-sensitivity and epithelial phenotype restoration. There is intra-tumoral variability in the tissue expression of miR-200 family members with decreased expression at the invasive front. Clinical studies in CRC patients have shown decreased primary tumor tissue expression of miR-429, miR-200a and miR-200c may be associated with worse survival. Conversely, increased blood levels of miR-141, miR-200a and miR-200c may be associated with worse outcomes. The miR-200 family has a central role in EMT. The miR200 family has potential for both prognostic and therapeutic management of CRC.

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“…Miyoshi et al, 6 who reported miR-139-5p upregulation in the serum samples of recurrence-positive CRC patients, added 25 fmol of cel-miR-39 to each sample for data normalization. Maierthaler et al, 10 who revealed that increased plasma levels of miR-122 were associated with a poor prognosis and a shorter survival in CRC patients, added 3.5 µL of cel-miR-39 (1.6 × 10 8 copies/µL) to plasma samples for data correction. Nonaka et al, 8 who identified miR-199a-3p as a novel serum biomarker for CRC, added 20 fmol of cel-miR-39 to each sample to manage sample-to-sample variations.…”

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confidence: 99%