Plasma Metabolomic Profiling after Feeding Dried Distiller’s Grains with Solubles in Different Cattle Breeds

Abstract

Dried distiller’s grains with solubles (DDGS) are rich in nutrients and can enhance animals’ growth and immunity. However, there are few reports on the effects of a diet of DDGS on plasma metabolism and the related action pathways in domestic animals. In this study, groups of Guanling yellow cattle (GY) and Guanling crossbred cattle (GC) having a basal diet served as the control groups (GY-CG and GC-CG), and DDGS replacing 25% of the diet of GY and GC served as the replacement groups (GY-RG and GC-RG), with three cattle in each group. Plasma samples were prepared for metabolomic analysis. Based on multivariate statistical and univariate analyses, differential metabolites and metabolic pathways were explored. Twenty-nine significantly different metabolites (p < 0.05) were screened in GY-RG compared with those in GY-CG and were found to be enriched in the metabolic pathways, including choline metabolism in cancer, linolenic acid metabolism, and amino acid metabolism. Nine metabolites showed significant differences (p < 0.05) between GC-RG and GC-CG and were mainly distributed in the metabolic pathways of choline metabolism in cancer, glycerophospholipid metabolism, prostate cancer metabolism, and gonadotropin-releasing hormone (GnRH) secretion. These results suggest that a DDGS diet may promote healthy growth and development of experimental cattle by modulating these metabolic pathways. Our findings not only shed light on the nutritional effects of the DDGS diet and its underlying mechanisms related to metabolism but also provide scientific reference for the feed utilization of DDGS.

Effect of feeding a dried distillers’ grains with solubles diet on the metabolism of the intestinal wall in Guanling crossbred cattle: a preliminary assessment

“…Improper disposal of fresh DGs easily causes waste of resources and environmental pollution, including soil contamination. Reasonable reuse of DGs as feed resources in livestock production is necessary ( 19 ). Drying DGs to obtain DDGS is a common strategy for reuse of DGs.…”

The effect of feeding fermented distillers' grains diet on the intestinal metabolic profile of Guanling crossbred cattle

“…All the samples were subjected to ultrasonic extraction for 10 min, stored at −20 • C for 30 min, and then centrifuged at 13,000 × g for 10 min at 4 • C. A total of 300 µL of supernatants were dried in a freezeconcentration centrifugal dryer and resolubilized by 200 µL of methanol/water (1/4), vortexed for 30 s, and subjected to ultrasonic extraction for 3 min. After placement for 2 h at −20 • C, the samples were centrifuged at 4 • C (13,000 × g) for 10 min, and 150 µL of supernatants were filtered through 0.22-µm microfilters and then transferred to liquid chromatography (LC) vials (22).…”

Immunotherapy and Dietary Selection

“…However, except for the conventional cancer treatment, dietary intervention has been reported to contribute in rebuilding the immunology of cancer patient. Therefore, apply the dietary intervention wisely for cancer patient is also important for increasing clinical benefits of patients [3,4].…”